Increased marble-burying behavior in ethanol-withdrawal state: Modulation by gonadotropin-releasing hormone agonist

Umathe, Sudhir N.; Bhutada, Pravinkumar; Dixit, Pankaj; Shende, Varsha

doi:10.1016/j.ejphar.2008.03.035

Cited by 39 publications

(26 citation statements)

References 49 publications

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“…Both our short-term ethanol injection treatment and liquid ethanol diet were sufficient to produce increases in compulsive-like behavior during withdrawal. Our results are comparable to previous findings in the MBT (Umathe et al, 2008). In addition, we show that this behavioral test can be used with both male and female mice.…”

Section: Discussionsupporting

confidence: 92%

“…The main finding of this report is that a relatively short ethanol injection regimen, when combined with an alcohol dehydrogenase inhibitor, can produce withdrawal-associated behaviors that are similar to those observed upon withdrawal from long-term treatment of mice with ethanol liquid diet (Kliethermes, 2005; Umathe et al, 2008; Uzbay et al, 1997). In addition to replicating previously reported increases in anxiety-like behavior in the EPM during ethanol withdrawal after chronic ethanol injections (Farook et al, 2007), our study also demonstrated that our shot-term alcohol treatment can produce increased anxiety-like behavior in the OFA.…”

Section: Discussionmentioning

confidence: 68%

“…Elevated compulsive-like behavior has been shown to occur in male mice during ethanol withdrawal in the MBT (Umathe et al, 2008). Both our short-term ethanol injection treatment and liquid ethanol diet were sufficient to produce increases in compulsive-like behavior during withdrawal.…”

Section: Discussionmentioning

confidence: 99%

“…Liquid diets have extensively been used in rodents to produce ethanol dependence and physiological changes associated with chronic ethanol use (Gilpin et al, 2008; Macey et al, 1996; Nan et al, 2013; Umathe, Bhutada, Dixit, & Shende, 2008; Verleye, Heulard, & Gillardin, 2009; Zhang et al, 2013). Mice were single-housed with a nestlet, and provided for one week with a basic diet consisting of the chocolate flavored high protein nutritional drink, Boost ® (Nestlé Health Science, Switzerland), supplemented with 3g/L vitamin mixture (MP Biomedicals, LLC, Solon, OH) and 5g/L mineral mix (ICN Biomedicals, Inc., Aurora, OH) (Gilpin et al, 2008; Verleye et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…Ethanol withdrawal-induced compulsive-like behavior was measured using the marble burying test (Umathe et al, 2008). Mice were placed for 30 min in a cage (26.5 cm × 15.5 cm × 12.5 cm) that contained 5 cm of corn cob bedding with 20 marbles (13 mm diameter) evenly spaced throughout the cage.…”

Section: Methodsmentioning

confidence: 99%

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Assessment of affective and somatic signs of ethanol withdrawal in C57BL/6J mice using a short-term ethanol treatment

Perez

Biasi

2015

Alcohol

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Alcohol is one of the most prevalent addictive substances in the world. Withdrawal symptoms result from abrupt cessation of alcohol consumption in habitual drinkers. The emergence of both affective and physical symptoms produces a state that promotes relapse. Mice provide a preclinical model that could be used to study alcohol dependence and withdrawal while controlling for both genetic and environmental variables. The use of a liquid ethanol diet offers a reliable method for the induction of alcohol dependence in mice, but this approach is impractical when conducting high throughput pharmacological screens or when comparing multiple strains of genetically engineered mice. The goal of this study was to compare withdrawal associated behaviors in mice chronically treated with a liquid ethanol diet vs. mice treated with a short-term ethanol treatment that consisted of daily ethanol injections containing the alcohol dehydrogenase inhibitor, 4-methylpyrazole. Twenty-four hours after ethanol treatment, mice were tested in the open field arena, the elevated plus maze, the marble burying test or for changes in somatic signs during spontaneous ethanol withdrawal. Anxiety-like and compulsive-like behavior, as well as physical signs, were all significantly elevated in mice undergoing withdrawal, regardless of the route of ethanol administration. Therefore, a short-term ethanol treatment can be utilized as a screening tool for testing genetic and pharmacological agents before investing in a more time consuming ethanol treatment.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Assessment of affective and somatic signs of ethanol withdrawal in C57BL/6J mice using a short-term ethanol treatment

Perez

Biasi

2015

Alcohol

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Central norepinephrine transmission is required for stress-induced repetitive behavior in two rodent models of obsessive-compulsive disorder

et al. 2020

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Noradrenergic tone mediates marble burying behavior after chronic stress and ethanol

et al. 2020

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Rationale-Stress plays a major role in the development of alcohol use disorder (AUD) -a history of chronic stress contributes to alcohol misuse, and withdrawal from alcohol elevates stress, perpetuating cycles of problematic drinking. Recent studies have shown that, in male mice, repeated chronic intermittent ethanol (CIE) and stress elevates alcohol use above either manipulation alone, and impacts cognitive functions such as behavioral flexibility.Objective-Here we investigated the impact of CIE and stress on anxiety in both sexes, and whether the norepinephrine (NE) system via locus coeruleus, which is implicated in both stress and alcohol motivation, is involved.Results-Male and female mice received multiple cycles of CIE and/or repeated forced swim stress (FSS), producing elevated drinking in both sexes. CIE/FSS treatment increased anxiety, which was blocked by treatment with the α1-AR inverse agonist prazosin. In contrast, administration of the corticotropin releasing factor receptor antagonist CP376395 into locus coeruleus did not reduce CIE/FSS elevated anxiety. We also observed sex differences in behavioral responses to a history of CIE or FSS alone as well as differential behavioral consequences of prazosin treatment.Conclusions-These data indicate that NE contributes to the development of anxiety following a history of alcohol and/or stress, and that the influence of both treatment history and NE signaling is sex dependent. These results argue for further investigation of the NE system in relation to disrupted behavior following chronic alcohol and stress, and support the assertion that treatments may differ across sex based on differential neural system engagement.Terms of use and reuse: academic research for non-commercial purposes, see here for full terms. http://www.springer.com/gb/openaccess/authors-rights/aam-terms-v1

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Increased marble-burying behavior in ethanol-withdrawal state: Modulation by gonadotropin-releasing hormone agonist

Cited by 39 publications

References 49 publications

Assessment of affective and somatic signs of ethanol withdrawal in C57BL/6J mice using a short-term ethanol treatment

Assessment of affective and somatic signs of ethanol withdrawal in C57BL/6J mice using a short-term ethanol treatment

Central norepinephrine transmission is required for stress-induced repetitive behavior in two rodent models of obsessive-compulsive disorder

Noradrenergic tone mediates marble burying behavior after chronic stress and ethanol

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