Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs

Monteiro, Lummy Maria Oliveira; Vici, Ana Claudia; Messias, Josana Maria; Heinen, Paulo Ricardo; Pinheiro, Vanessa Elisa; Vargas-Rechia, Carem Gledes; Buckeridge, Marcos Silveira; Polizeli, Maria de Lourdes Teixeira de Moraes

doi:10.1016/j.btre.2021.e00618

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…Central composite design (CCD) and response surface analysis are considered powerful and valuable strategies to optimize biological processes; it is also timesaving related to the traditional 'one-factor-at-a-time,' helping to reduce costs in the enzymatic process [27] effectively. Therefore, we applied this statistical design to optimize laccase pretreatment, studying laccase load (U g −1 ), mediator concentration (w/v%), and temperature ( • C) as independent variables.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions

Freitas

Alnoch

Contato

et al. 2021

IJMS

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Since laccase acts specifically in lignin, the major contributor to biomass recalcitrance, this biocatalyst represents an important alternative to the pretreatment of lignocellulosic biomass. Therefore, this study investigates the laccase pretreatment and climate change effects on the hydrolytic performance of Panicum maximum. Through a Trop-T-FACE system, P. maximum grew under current (Control (C)) and future climate conditions: elevated temperature (2 °C more than the ambient canopy temperature) combined with elevated atmospheric CO2 concentration(600 μmol mol−1), name as eT+eC. Pretreatment using a laccase-rich crude extract from Lentinus sajor caju was optimized through statistical strategies, resulting in an increase in the sugar yield of P. maximum biomass (up to 57%) comparing to non-treated biomass and enabling hydrolysis at higher solid loading, achieving up to 26 g L−1. These increments are related to lignin removal (up to 46%) and lignin hydrophilization catalyzed by laccase. Results from SEM, CLSM, FTIR, and GC-MS supported the laccase-catalyzed lignin removal. Moreover, laccase mitigates climate effects, and no significant differences in hydrolytic potential were found between C and eT+eC groups. This study shows that crude laccase pretreatment is a potential and sustainable method for biorefinery solutions and helped establish P. maximum as a promising energy crop.

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Section: Discussionmentioning

confidence: 99%

Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions

Freitas

Alnoch

Contato

et al. 2021

IJMS

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“…The model fit coefficient was n (R 2 ) 0.9960 with a high F-value. Cellobiose emerged as the optimal carbon source, yielding an enzyme with remarkable performance, boasting a relative activity of 100% and an enzyme activity level of 1.55 U/mg [ 45 ]. Another study focused on the production of β-glucosidase by utilizing the microorganism Aspergillus fumigatus strain isolated from the Atlantic Forest biome.…”

Section: Upstream Bioprocessingmentioning

confidence: 99%

Section: Enzyme Productionmentioning

confidence: 99%

Upstream and Downstream Bioprocessing in Enzyme Technology

Guajardo,

Schrebler

2023

Pharmaceutics

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The development of biotransformation must integrate upstream and downstream processes. Upstream bioprocessing will influence downstream bioprocessing. It is essential to consider this because downstream processes can constitute the highest cost in bioprocessing. This review comprehensively overviews the most critical aspects of upstream and downstream bioprocessing in enzymatic biocatalysis. The main upstream processes discussed are enzyme production, enzyme immobilization methodologies, solvent selection, and statistical optimization methodologies. The main downstream processes reviewed in this work are biocatalyst recovery and product separation and purification. The correct selection and combination of upstream and downstream methodologies will allow the development of a sustainable and highly productive system.

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“…In general, these systems are produced and secreted by microorganisms, mainly filamentous fungi, which have developed cellular secretory mechanisms. Several biomasses have been reported as potentially producing bioenergy, such as sugarcane bagasse [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ], corn residues [ 16 , 17 ], paper sludge, and eucalyptus chips [ 18 , 19 ]. This review provides an overview of the impacts of plant responses to abiotic stress in cell wall properties and biomass digestibility, reporting the key challenges to be faced on the use of lignocellulosic feedstocks for bioenergy purposes in climate change scenarios.…”

Section: Introductionmentioning

confidence: 99%

Challenges of Biomass Utilization for Bioenergy in a Climate Change Scenario

Freitas

Salgado

Alnoch

et al. 2021

Biology

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The climate changes expected for the next decades will expose plants to increasing occurrences of combined abiotic stresses, including drought, higher temperatures, and elevated CO2 atmospheric concentrations. These abiotic stresses have significant consequences on photosynthesis and other plants’ physiological processes and can lead to tolerance mechanisms that impact metabolism dynamics and limit plant productivity. Furthermore, due to the high carbohydrate content on the cell wall, plants represent a an essential source of lignocellulosic biomass for biofuels production. Thus, it is necessary to estimate their potential as feedstock for renewable energy production in future climate conditions since the synthesis of cell wall components seems to be affected by abiotic stresses. This review provides a brief overview of plant responses and the tolerance mechanisms applied in climate change scenarios that could impact its use as lignocellulosic biomass for bioenergy purposes. Important steps of biofuel production, which might influence the effects of climate change, besides biomass pretreatments and enzymatic biochemical conversions, are also discussed. We believe that this study may improve our understanding of the plant biological adaptations to combined abiotic stress and assist in the decision-making for selecting key agronomic crops that can be efficiently adapted to climate changes and applied in bioenergy production.

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Increased Malbranchea pulchella β-glucosidase production and its application in agroindustrial residue hydrolysis: A research based on experimental designs

Abstract: Graphical abstract

Cited by 8 publications

References 52 publications

Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions

Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions

Upstream and Downstream Bioprocessing in Enzyme Technology

Challenges of Biomass Utilization for Bioenergy in a Climate Change Scenario

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