Increased lymphocyte subpopulations and macrophages in the ovaries and oviducts of laying hens infected with<i>Salmonella enterica</i>serovar Enteritidis

Withanage, G. S. K.; Sasai, Kazumi; Fukata, Tsuneo; Miyamoto, Tadashi; Lillehoj, H. S.; Baba, Eiichiroh

doi:10.1080/03079450310001610631

Cited by 38 publications

(19 citation statements)

References 24 publications

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“…For CD8 + cells, we found an increase after the Salmonella infection in both chicken lines, but faster in time post infection in line F. An increase in the amount of CD8 + cells was also found in the caeca after a Salmonella infection [1,6], but also decreases in the amount of CD8+ cells in the caeca are reported [18]. In the oviduct of laying hens, the numbers of CD8 + cells were increased after a Salmonella infection [4,25,26]. Our and other results suggest that differences in influx of T-cell subpopulations after a Salmonella infection are dependent on the location in the digestive tract, infection dose, time post infection, age at the time of infection and genetic background of the chickens.…”

Section: Discussionmentioning

confidence: 53%

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Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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-Besides infection in humans, Salmonella enteritidis can also cause serious illness in young chickens. However, the genetic and immunological parameters important for the disease in chickens are not well characterized. In this study, processes in the chicken intestine in response to a Salmonella infection were investigated in two different chicken lines. One-day-old chickens were orally infected with Salmonella. T-cell subpopulations, phagocytic properties of intestinal mononuclear cells and RNA expression levels of the jejunum were investigated. The two chicken lines differed in the amount of cfu in the liver and growth retardation after the infection. Differences in phagocytic activity of intestinal mononuclear cells were found between control and Salmonella infected chickens. The number of CD4 + T-cells of the intestine decreased after the Salmonella infection in one chicken line, while the number of CD8 + T-cells increased in both chicken lines, but the time post infection of this increase differed between the lines. In one chicken line the expression levels of the genes carboxypeptidase M and similar to ORF2 decreased after the Salmonella infection, which might be related to a decrease in the amount of macrophages. With the microarray, ten genes were found that were regulated in only one of the chicken lines, while we found six genes regulated in response to the infection in both chicken lines. So differences in genetic background of the chickens influence the intestinal host response of the Salmonella infection as observed by phagocytic activity, gene expression and changes in the number of T-cell subpopulations and macrophages.immunology / microarray / Salmonella / T-cell

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Section: Discussionmentioning

confidence: 53%

“…Also in mice the number of CD4 + T-cells increased in the gut following Salmonella challenge [2,15]. Furthermore in the ovary and oviduct of laying hens, increased numbers of CD4 + T-cells were reported after a Salmonella infection [4,25,26]. However, for the early host response CD4 + or CD8 + T-cells are probably not required [14].…”

Section: Discussionmentioning

confidence: 99%

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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“…An increased level of IL-1β stimulates infiltration of T cells and macrophages [42]. Treatment of birds with SE causes infiltration of these immunocompetent cells in ovarian follicles [5,7,43]. We assume that TLR-4 plays a significant role in recognition of SE to enhance IL-1β expression, leading to influx of immunocompetent cells.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Expression of Toll-Like Receptor mRNAs During Follicular Growth and in Response to Lipopolysaccharide in the Ovarian Follicles of Laying Hens

Subedi

Isobe

Nishibori

et al. 2007

Journal of Reproduction and Development

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Abstract. The aim of this study was to determine the changes in the mRNA expression of Toll-like receptors (TLRs) in hen ovarian follicles during follicular growth and in response to lipopolysaccharide (LPS). White follicles and the fifth largest to largest follicles (WF and F5-F1, respectively) were collected from laying hens. To examine the effects of LPS, the laying hens were treated intravenously with LPS (1 mg/kg BW) 0, 3, 6, 12 and 24 h before examination. Expressions of TLRs and IL-1β in the theca and granulosa layers were examined by semi-quantitative RT-PCR. Immunocytochemistry was performed to identify immunoreactive TLR-4. The theca layer expressed TLR-2, TLR-4, TLR-5 and TLR-7, whereas the granulosa layer expressed only TLR-4 and TLR-5. The expression of TLR-4 and TLR-5 in the theca layer increased significantly during follicular growth. In the granulosa layer, the expression of TLR-5 increased, but that of TLR-4 was unchanged. Expression of TLR-4 increased significantly during the period of 6 to 12 h of LPS treatment in the theca layer and during the period of 12 to 24 h in the granulosa layer of F3. Immunoreaction products for TLR-4 were observed in theca interna and granulosa layers of WF and F5-F1, with the greater amount observed in the theca interna. LPS treatment significantly increased expression of IL-1β in the theca layer after 3 h and in the granulosa layer during the period of 12 to 24 h. These results suggest that TLRs are expressed in ovarian follicles and that TLR-4 and TLR-5 expression increase with the growth of follicles. Enhanced expression of TLR-4 and IL-1β by LPS in the theca and granulosa layers suggests possible roles of TLR in recognition of microorganisms.

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“…T cell subsets play essential roles in the defense against viral and bacterial infection (Janse et al, 1994;Berndt and Methner, 2001;Beal et al, 2004), and CD4 + and CD8 + T cells were increased in hen oviduct infected by Salmonella enteritidis (Withanage et al, 1998(Withanage et al, , 2003. The current study showed that repeated stimulation by antigen increased the density of CD4 + , CD8…”

Section: Of B Cells and Macrophages By Cd4mentioning

confidence: 51%

Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina

2013

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The aim of this study was to determine how the mucosal immunity mediated by T cells is developed in the vagina. Antigen-presenting cells and T cells play important roles in the early process of host defense. Mature T cells and immature dendritic cells expressing CC chemokine receptor (CCR) 6. In contrast, immature T cells and mature dendritic cells express CCR7. It was examined whether repeated antigen stimulation is effective for development of the pool of antigen-presenting cells and T cells in the vagina. White Leghorn hens were intravaginally injected 1 (single injection group) or 5 times (repeated injection group) in 10 days with saline (control) or lipopolysaccharide (LPS). The vagina was collected 1 day after the final injection. , TCRγδ+ T cells were significantly increased in LPS-repeated injection group, however that of MHC class II + cells was not changed significantly. The expression of CCR6 was significantly upregulated in LPS-repeated injection group, however, the expression of CCR7 and MHC class II was not affected. The densities of the each T cell subset and MHC class II + cells, as well as expressions of chemokine receptor, MHC class II were not affected by single LPS injection or saline injection. These results suggest that the repeated antigen stimulation may not affect antigen-presenting ability by antigen-presenting cells, whereas it may lead influx of T cell subsets in the vagina. These accumulated T cells pool may contribute to form prequiescence of host immunity in the vaginal mucosa.

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Increased lymphocyte subpopulations and macrophages in the ovaries and oviducts of laying hens infected withSalmonella entericaserovar Enteritidis

Cited by 38 publications

References 24 publications

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Changes in the Expression of Toll-Like Receptor mRNAs During Follicular Growth and in Response to Lipopolysaccharide in the Ovarian Follicles of Laying Hens

Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina

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