Increased lipoperoxide levels and antioxidant system in colorectal cancer

Abstract

In this study, lipid peroxide and glutathione (GSH) levels, GSH peroxidase, GSH S-transferase, superoxide dismutase, gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase activities were investigated in tumorous and nontumorous colorectal tissues obtained from ten patients diagnosed with colon and rectum cancer. Histopathological evaluations, including type, stage, necrosis and lymphocyte infiltration, were also performed for each patient. According to the results, lipid peroxide and GSH levels and the activities of GSH peroxidase, superoxide dismutase, gamma-glutamylcysteine synthetase were found to be increased, while GSH S-transferase and gamma-glutamyl transpeptidase activities remained unchanged in tumors compared to adjacent normal tissues of subjects with colorectal cancer. However, the considerable interindividual variations were found in these parameters. A definite interrelation between histopathological results with lipid peroxidation and antioxidant system was not observed.

Oxidant-antioxidant status in colorectal cancer patients—Before and after treatment

“…The GST levels in tumor tissue of colorectal cancer were similar to the GST levels in the normal colorectal tissue of subjects with colorectal cancer (17). No significant difference in activity of GST was observed in human breast cancer tumor cell line and Adriamycin resistant cell line.…”

“…However MDA levels have been reported to be higher in patients with malignant breast tumor (15, *p < 0.0001 compared to controls **p = 0.05 compared to controls. ns -not significant when compared to controls (p > 0.05) NS -not significant when compared to pre treatment (p 9 0.05) 16), colorectal cancer tissue (17) and in erythrocytes of rats with hepatoma (9).…”

Expression and potential role of apolipoprotein D on the death–survival balance of human colorectal cancer cells under oxidative stress conditions

“…An increase in lipid peroxidation, both in blood and cancerous tissue samples, has been previously reported in primary CRC patients [39][40][41]. In particular, Skrzydlewska et al [41] found a steady increase of the lipid peroxide adduct MDA in malignant tissue of stages II-IV and a sustained increase in 4-HNE.…”

The Effect of n-6 Polyunsaturated Fatty Acid on Blood Levels of Malondialdehyde-Deoxyguanosine Adducts in Human Subjects

“…There is, however, known to be a large geographical difference in the incidence of colorectal cancer with the highest number of cases in western countries such as Europe and the USA, whilst studies in migrant populations have shown that colorectal cancer incidence increases upon changing to a western diet [4][5][6], although changes in other lifestyle factors such as physical activity levels have also been implicated. Dietary fat, lipid peroxidation (LPO) and arachidonic acid metabolism have all been linked, amongst other factors, to colorectal carcinogenesis in a number of studies [7][8][9]. LPO is initiated by the attack of free-radicals on membrane lipids leading to reactive products that may be linked to tumour initiation.…”