Increased lipid peroxidation in adult GH-deficient patients: Effects of short-term GH administration

Scacchi, Massimo; Valassi, Elena; Pincelli, Angela Ida; Fatti, Letizia Maria; Giraldi, Francesco; Ascoli, Paola; Viarengo, R.; Cestaro, Benvenuto; Cavagnini, Francesco; Cazzola, Roberta

doi:10.1007/bf03349194

Cited by 19 publications

(17 citation statements)

References 21 publications

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“…1 mg/kg GH was able to exert beneficial effects enhancing antioxidant defences, reducing oxidative stress and improving energy generation in the myocardium of rats with heart failure (Seiva et al 2008). Also, GH replacement was able to partially reverse the abnormally increased LPO (KarbownikLewinska et al 2008) and to significantly prolong lag-time (index of anti-oxidant activity) in GHdeficient adults (Scacchi et al 2006). Previous studies from our group have shown that GH could also improve some parameters related to the oxidative damage (LPO, NO levels) associated with aging in hepatocytes isolated from old female and male rats (Castillo et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

et al. 2009

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There is now a large body of evidence suggesting that the decline in ovarian function with menopause is associated with spontaneous increases in pro-inflammatory cytokines. On the other hand, oxidative stress has been implicated in the pathogenesis of several alterations due to menopause, and can arise through the increased production of lipid peroxides (LPO) and/or a deficiency of antioxidant defense. The aim of the present study was to investigate the effect of aging and ovariectomy on various physiological parameters related to inflammation and oxidative stress in livers obtained from old female rats and the influence of chronic exogenous administration of estrogens, phytoestrogens and growth hormone on these. Thirty-six female Wistar rats of 22 months of age were used in the present study. Twelve of them remained intact, and the other 24 had been ovariectomized at 12 months of age. Intact animals were divided into two groups and treated for 10 weeks with GH or saline, and ovariectomized animals were divided into four groups and treated for the same time with GH, estrogens, phytoestrogens or saline. A group of 2 month old intact female rats was used as young control. Protein expression of iNOS, HO-1, IL-6, TNFalpha, and IL-1beta were determined by Western blot analysis. The levels of NO( x ), LPO, TNFalpha, IL-1beta, IL-6 and IL-10 were determined in different fractions of the liver. Levels of LPO in the liver homogenates as well as iNOS protein expression and NO( x ) levels were increased in old rats as compared to young animals; this effect was more evident in ovariectomized animals. Pro-inflammatory cytokines TNF-alpha, IL-1beta and IL-6 were significantly increased and anti-inflammatory IL-10 decreased during ageing and after ovariectomy. Aging also significantly increased expression of HO-1 protein and ovariectomized rats showed an additional increase. Hormonal administration to the ovariectomized groups decreased NO( x ), LPO levels and pro-inflammatory cytokines as compared with untreated rats. Significant rise in IL-10 and reductions in the iNOS, IL-6, TNFalpha and IL-1beta proteins expression were also found. Oxidative stress and inflammation induced during aging in the liver are more marked in castrated than in intact old females. Administration of the different hormonal replacement therapies was able to inhibit the induction of pro-inflammatory cytokines and iNOS, decreased the levels of oxidative stress markers and had therapeutic potential in the prevention of liver injury.

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Section: Discussionmentioning

confidence: 99%

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

et al. 2009

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“…The increased blood LPO level in GH-deficient adults has been reported 10 and confirmed in the present study. Other authors also found increased oxidative damage to lipids [7][8][9] or to DNA in GHdeficient patients. 11 Such findings justify the conclusion that redox imbalance exists in GH-deficient patients.…”

Section: Discussionmentioning

confidence: 87%

“…In the first one, 4 months of GH replacement decreased plasma lipid peroxide concentration, however, the parameter, measured in that study, represents oxidative damage to LDL-cholesterol exclusively. 9 In the second, 6 months of GH replacement decreased oxidized guanosine (the product of oxidative DNA damage) urine excretion described in a report of a single investigated patient. 11 Both of these parameters differ from those evaluated in the present study.…”

Section: Discussionmentioning

confidence: 94%

GH replacement reduces increased lipid peroxidation in GH‐deficient adults

Karbownik-Lewińska

Kokoszko

Lewandowski

et al. 2007

Clinical Endocrinology

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“…In this regard, after 24 weeks of GH replacement therapy in the GREAT study, the hormone significantly lowered plasma diacron-reactive oxygen metabolites and improved endothelial function, as measured by reactive hyperemia index [145]. This indicates that GH can exert a protective role in redox balance in GHD, in which predominates a pro-oxidant environment, corrected by short-term GH administration [146]. Klotho, a GH-releasing factor that currently is gaining in interest, also lowers the oxidative stress, decreasing apoptosis and senescence of the vascular system in an atherogenic risk rat model [147].…”

Section: Cellular Senescence and Wound Healing: Benefit Of Gh Therapymentioning

confidence: 92%

Growth Hormone (GH) and Wound Healing

Caicedo¹,

Devesa²

2019

Wound Healing - Current Perspectives

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Wound healing is complex and numerous factors overlap perfectly with the goal of wound closure. Among them, we will focus on a large amount of experimental and clinical evidence on the action of GH in wound repair. We will analyze how the physiological rhythm of GH secretion influences this process, and also one of the most important signaling pathways that mediate the effects of GH on tissue regeneration. The role of IGF-1 and the factors that stimulate GH secretion and that have also been shown to improve healing will also be reviewed. In addition, it will be analyzed the cellular senescence process, which plays a key role in nonhealing wounds associated with chronic diseases. The benefit of GH in this last circumstance is especially important. The lesions associated with catabolic states, mainly burns, are considered a delicate situation in which it is extraordinarily difficult to act with growth factors due to the fragile situation of these patients, often children. The positive action of GH in these states will also be described. In summary, we will analyze many evidences about the beneficial effects of GH and its main secretagogues in the healing of wounds.

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Increased lipid peroxidation in adult GH-deficient patients: Effects of short-term GH administration

Cited by 19 publications

References 21 publications

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomized female rats

GH replacement reduces increased lipid peroxidation in GH‐deficient adults

Growth Hormone (GH) and Wound Healing

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