Increased linoleic acid/α-linolenic acid ratio in Swedish cord blood samples collected between 1985 and 2005

Warstedt, Kristina; Duchén, Karel

doi:10.1007/s00394-012-0369-6

Cited by 4 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, our study could not find differences between atopic and nonatopic mothers as previously reported in a similar research setting. This may be explained by either the small sample size among the mothers receiving the placebo (n = 53) or changes in maternal dietary consumption of omega‐3 PUFA over time .…”

Section: Discussionmentioning

confidence: 99%

High levels of omega‐3 fatty acids in milk from omega‐3 fatty acid‐supplemented mothers are related to less immunoglobulin E‐associated disease in infancy

et al. 2016

Self Cite

View full text Add to dashboard Cite

Omega-3 milk fatty acids were higher in women who received omega-3 supplements than the placebo group (p < 0.01). Higher proportions of milk eicosapentaenoic acid and docosahexaenoic acid and a lower arachidonic/eicosapentaenoic acid ratio were associated with an absence of IgE-associated disease in the infants. None of the children developed IgE-associated atopic eczema above a level of 0.83 mol% eicosapentaenoic acid in colostrum. [Correction added on 7 July 2016, after online publication: In the preceding sentence, the correct word should be "above" instead of "below" and this has been amended in this current version.] CONCLUSION: High omega-3 LCPUFA milk levels in mothers who received omega-3 LCPUFA supplements were related to fewer allergies in their children.

show abstract

Section: Discussionmentioning

confidence: 99%

High levels of omega‐3 fatty acids in milk from omega‐3 fatty acid‐supplemented mothers are related to less immunoglobulin E‐associated disease in infancy

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…By supplementing pregnant women, with a high propensity of having an allergic child, during pregnancy and lactation with ω-3 fatty acids and probiotics, it may be possible to modulate the foetal immune system towards tolerance [ 8 , 10 , 11 ]. In Westernised societies the dietary fatty acid intake favours ω-6 over ω-3 fatty acids [ 12 , 13 ], thereby promoting production of pro-inflammatory mediators, such as prostaglandins and leukotrienes [ 14 ], and possibly sustaining allergic inflammation [ 11 ]. However, by ω-3 supplementation this ratio may be altered and promote downstream production of immunoregulatory mediators [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Combined prenatal Lactobacillus reuteri and ω-3 supplementation synergistically modulates DNA methylation in neonatal T helper cells

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Environmental exposures may alter DNA methylation patterns of T helper cells. As T helper cells are instrumental for allergy development, changes in methylation patterns may constitute a mechanism of action for allergy preventive interventions. While epigenetic effects of separate perinatal probiotic or ω-3 fatty acid supplementation have been studied previously, the combined treatment has not been assessed. We aimed to investigate epigenome-wide DNA methylation patterns from a sub-group of children in an on-going randomised double-blind placebo-controlled allergy prevention trial using pre- and postnatal combined Lactobacillus reuteri and ω-3 fatty acid treatment. To this end, > 866000 CpG sites (MethylationEPIC 850K array) in cord blood CD4+ T cells were examined in samples from all four study arms (double-treatment: n = 18, single treatments: probiotics n = 16, ω-3 n = 15, and double placebo: n = 14). Statistical and bioinformatic analyses identified treatment-associated differentially methylated CpGs and genes, which were used to identify putatively treatment-induced network modules. Pathway analyses inferred biological relevance, and comparisons were made to an independent allergy data set. Results Comparing the active treatments to the double placebo group, most differentially methylated CpGs and genes were hypermethylated, possibly suggesting induction of transcriptional inhibition. The double-treated group showed the largest number of differentially methylated CpGs, of which many were unique, suggesting synergy between interventions. Clusters within the double-treated network module consisted of immune-related pathways, including T cell receptor signalling, and antigen processing and presentation, with similar pathways revealed for the single-treatment modules. CpGs derived from differential methylation and network module analyses were enriched in an independent allergy data set, particularly in the double-treatment group, proposing treatment-induced DNA methylation changes as relevant for allergy development. Conclusion Prenatal L. reuteri and/or ω-3 fatty acid treatment results in hypermethylation and affects immune- and allergy-related pathways in neonatal T helper cells, with potentially synergistic effects between the interventions and relevance for allergic disease. Further studies need to address these findings on a transcriptional level, and whether the results associate to allergy development in the children. Understanding the role of DNA methylation in regulating effects of perinatal probiotic and ω-3 interventions may provide essential knowledge in the development of efficacious allergy preventive strategies. Trial registration ClinicalTrials.gov, ClinicalTrials.gov-ID: NCT01542970. Registered 27th of February 2012—Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT01542970.

show abstract

“…7,8 By supplementing pregnant women, with a high propensity of having an allergic child, during pregnancy and lactation with ω-3 fatty acids and probiotics, it may be possible to modulate the foetal immune system towards tolerance. 7,9,10 In Westernised societies the dietary fatty acid intake favours ω-6 over ω-3 fatty acids 11,12 , thereby promoting production of pro-inflammatory mediators, such as prostaglandins and leukotrienes 13 , and possibly sustaining allergic inflammation. 10 However, by ω-3 supplementation this ratio may be altered and promote downstream production of immunoregulatory mediators.…”

Section: Introductionmentioning

confidence: 99%

Combined prenatal Lactobacillus reuteri and ω-3 supplementation synergistically modulates DNA methylation in neonatal T helper cells

Huoman

Martínez-Enguita

Olsson

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Environmental exposures may alter DNA methylation patterns of T helper cells. As T helper cells are instrumental for allergy development, changes in methylation patterns may constitute a mechanism of action for allergy preventive interventions. While epigenetic effects of separate perinatal probiotic or ω-3 fatty acid supplementation have been studied previously, the combined treatment has not been assessed. We aimed to investigate epigenome-wide DNA methylation patterns in cord blood samples from children in a randomised double-blind placebo-controlled allergy prevention trial using pre- and postnatal combined Lactobacillus reuteri and ω-3 fatty acid treatment. To this end, >866 000 CpG sites (MethylationEPIC 850K array) in cord blood CD4+ T cells were examined in samples from all four study arms (double-treatment: n=18, single treatments: probiotics n=16, ω-3 n=15, and double placebo: n=14). Statistical and bioinformatic analyses identified treatment-associated differentially methylated CpGs and genes, which were used to identify treatment-induced network modules. Pathway analyses inferred biological relevance, and comparisons were made to an independent allergy data set. Results: Comparing the active treatments to the double placebo group, most differentially methylated CpGs and genes were hypermethylated, suggesting induction of transcriptional inhibition. The double-treated group showed the largest number of differentially methylated CpGs, of which many were unique, suggesting synergy between interventions. Clusters within the double-treated network module consisted of immune-related pathways, including T cell receptor signalling, and antigen processing and presentation, with similar pathways revealed for the single-treatment modules. CpGs derived from differential methylation and network module analyses were enriched in an independent allergy data set, particularly in the double-treatment group, proposing treatment-induced DNA methylation changes as relevant for allergy development. Conclusion: Prenatal L. reuteri and/or ω-3 fatty acid treatment results in hypermethylation and affects immune- and allergy-related pathways in neonatal T helper cells, with potentially synergistic effects between the interventions and relevance for allergic disease. Further studies need to address these findings on a transcriptional level, and whether the results associate to allergy development in the children. Understanding the role of DNA methylation in regulating effects of perinatal probiotic and ω-3 interventions may provide essential knowledge in the development of efficacious allergy preventive strategies.

show abstract

Increased linoleic acid/α-linolenic acid ratio in Swedish cord blood samples collected between 1985 and 2005

Abstract: The LA/LNA ratio increased (p < 0.001) in cord serum samples collected between 1985 and 2005, and no correlations between fatty acids and total IgE were found.

Cited by 4 publications

References 33 publications

High levels of omega‐3 fatty acids in milk from omega‐3 fatty acid‐supplemented mothers are related to less immunoglobulin E‐associated disease in infancy

High levels of omega‐3 fatty acids in milk from omega‐3 fatty acid‐supplemented mothers are related to less immunoglobulin E‐associated disease in infancy

Combined prenatal Lactobacillus reuteri and ω-3 supplementation synergistically modulates DNA methylation in neonatal T helper cells

Combined prenatal Lactobacillus reuteri and ω-3 supplementation synergistically modulates DNA methylation in neonatal T helper cells

Contact Info

Product

Resources

About