Increased levels of interferon-gamma in seminal plasma of infertile men

Paradisi, Roberto; Capelli, M.; Mandini, M; Bellavia, Emanuela; Flamigni, Carlo

doi:10.1111/j.1439-0272.1996.tb02775.x

Cited by 29 publications

(13 citation statements)

References 15 publications

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“…In our study, it was possible to detect IFN‐γ in all the seminal plasma samples collected. This is in agreement with the data presented by others (Paradisi et al. , 1996).…”

Section: Discussionsupporting

confidence: 94%

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Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle

et al. 2011

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Cytokines are released by various immunocompetent cell subsets in the male urogenital tract and are thought to affect sperm cell function and reproductive process. The aim of the study was to evaluate the levels and a possible role of seven seminal plasma cytokines with fertilisation rates in men attending an in vitro fertilisation (IVF) programme. A total of 36 men of couples who were undergoing traditional IVF in a regional reproductive medicine unit were recruited into this prospective study. Cytokines such as interleukin (IL)-6, IL-8, IL-10, IL-11, IL-12, tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) in the seminal plasma were determined using enzyme linked immunosorbent assay. IL-6, IL-8, IL-10, IL-11 and IFN-γ were detected in all samples. IL-12, and TNF-α were detected in most samples. Levels of IL-11 were significantly higher in the good fertiliser group (P ≤ 0.05). Positive correlation between cytokines such as IL-6 and IL-8 (P < 0.03), IL-10 and IL-11 (P < 0.001) and IFN-γ and IL-10 and IL-11 (P < 0.04 and P < 0.0001 respectively) were found. Our study confirms that the six cytokines other than IL-11 do not affect spermatozoon-oocyte interaction and fertilisation rates in IVF. IL-11 could have a role in the fertilising capacity of the spermatozoa. Significant correlation exists among these cytokines which shows that cytokines rarely act in isolation but rather in a network.

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“…In our study, it was possible to detect IFN‐γ in all the seminal plasma samples collected. This is in agreement with the data presented by others (Paradisi et al. , 1996).…”

Section: Discussionsupporting

confidence: 94%

“…The previous research has shown no significant difference in IFN‐γ levels between fertile and infertile subjects. However, increased IFN‐γ concentrations in the seminal plasma of infertile subjects have also been found (Paradisi et al. , 1996).…”

Section: Discussionmentioning

confidence: 99%

Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle

et al. 2011

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“…Paradisi et al . () have shown that IFN‐γ secretion is increased in the infertile men. Zhang et al .…”

Section: Discussionmentioning

confidence: 98%

Differential expression of novel biomarkers (TLR-2, TLR-4, COX-2, and Nrf-2) of inflammation and oxidative stress in semen of leukocytospermia patients

et al. 2015

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SUMMARYChronic genitourinary inflammation results in Leukocytospermia (LCS), an elevated number of white blood cells (WBCs) in semen, which, in association with oxidative stress, may suppress sperm function, and manifest as male factor infertility. The current clinical diagnosis of LCS employs manual enumeration of WBCs and requires complex staining and laboratory skills or measurement of inflammatory cytokines and chemokines levels. Many patients with idiopathic infertility are asymptomatic. In search of better inflammatory markers for LCS, we evaluated expression of toll-like receptors 2 and 4 (TLR-2/4), cyclooxygenase-2 (COX-2), and nuclear factor (erythroid-derived 2)-like 2 (Nrf-2) in semen samples of age-matched infertile patients with and without LCS. We employed the usage of specific Western blot evaluation, cytokine array; immunofluorescence microscopy (IFM) followed by computer-based analysis, and other molecular approaches. As compared with non-LCS patients (n = 38), semen samples from LCS patients (n = 47) displayed significantly lower total sperm count (p < 0.01), motility (p < 0.0001), normal head count (p < 0.0001), and a significantly higher white blood cell count (p < 0.0001). Differential cytokine profiling of seminal plasma by antibody array revealed up-regulation of several pro-inflammatory chemokines in LCS samples. Western blot analysis of LCS seminal plasma (n = 15) also showed a significant increase in expression of TLR-2 (p < 0.001) and 4 (p < 0.01), COX-2 (p < 0.001), and Nrf-2 (p < 0.001) as compared with semen samples from non-LCS patients (n = 15). Computer-based objective IFM analysis of spermatozoa from LCS patients showed increased expression of TLR-4 (p < 0.001), Cox-2 (p < 0.01), and (Nrf-2) (p < 0.01). Significant differences in the subcellular localization of these proteins were evident in the sperm head and tail segments of LCS samples. Altogether, these observations suggest that TLR-2/4, COX-2, and Nrf-2 can serve as novel biomarkers of inflammation and oxidative stress. Therefore, developing a rapid assay for these biomarkers may facilitate early diagnosis and management of LCS especially in idiopathic and asymptomatic male infertility patients.

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“…For instance, high concentrations of IFN-c, IL-17, MCP-1, MDC, IL-6 and IL-8 had been measured in SP samples from infertile men or having pathological processes in their genital tract. [17][18][19]27,28 Some other SP cytokines, such as IL-10, had shown contradictory results among studies. Huleihel et al (1999) reported lower concentrations of IL-10 in infertile than fertile men, whereas Miller et al (2002) reported opposite results.…”

Section: Discussionmentioning

confidence: 99%

The Seminal Plasma of the Boar is Rich in Cytokines, with Significant Individual and Intra-Ejaculate Variation

Barranco

Rubér

Pérez-Patiño

et al. 2015

Am J Reprod Immunol

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Problem: The boar, as human, sequentially ejaculates sperm-rich and sperm-poor fractions.Seminal plasma (SP) spermadhesins (PSP-I/PSP-II) induce a primary endometrial inflammatory response in female sows, similar to that elicited by semen deposition in other species, including human. However, the SP is also known to mitigate such response, making it transient to allow for embryo entry to a cleansed endometrium. Although cytokineinvolvement has been claimed, the exploration of cytokines in different SP-fractions is scarce. This study determines Th1, Th2, Th17 and Th3 cytokine profiles in specific ejaculate SP-fractions from boars of proven fertility. Conclusions: Boar SP is rich in Th1, Th2, Th17 and Th3 cytokines, with lowest concentrations in the sperm peak-containing fraction, indicating its main immune influence might reside in the larger, protein-rich sperm-poor post-SRF.

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Increased levels of interferon-gamma in seminal plasma of infertile men

Cited by 29 publications

References 15 publications

Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle

Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle

Differential expression of novel biomarkers (TLR-2, TLR-4, COX-2, and Nrf-2) of inflammation and oxidative stress in semen of leukocytospermia patients

The Seminal Plasma of the Boar is Rich in Cytokines, with Significant Individual and Intra-Ejaculate Variation

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