Increased Levels of Circulating Endothelial-Derived Microparticles and Small-Size Platelet-Derived Microparticles in Psoriasis

Pelletier, F.; Garnache‐Ottou, Francine; Angelot, Fanny; Biichlé, Sabéha; Vidal, Chrystelle; Humbert, Philippe; Saas, Philippe; Seillès, Estelle; Aubin, F.

doi:10.1038/jid.2011.57

Cited by 53 publications

(53 citation statements)

References 13 publications

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“…Thus increased PDMP concentrations observed in the psoriatic patients may be the contributory factor to development of atherosclerotic plaque by triggering a cascade of events. Our findings are in line with studies showing increased concentrations of PDMP in psoriatics as compared to controls [38]. PDMP are shown to increase leukocyte adhesion and activation by modulating leukocyte-leukocyte and leukocyte-endothelial cell interactions [39].…”

Section: Discussionsupporting

confidence: 93%

Is enhanced platelet activation the missing link leading to increased cardiovascular risk in psoriasis?

Chandrashekar

Rajappa

Revathy

et al. 2015

Clinica Chimica Acta

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Section: Discussionsupporting

confidence: 93%

Is enhanced platelet activation the missing link leading to increased cardiovascular risk in psoriasis?

Chandrashekar

Rajappa

Revathy

et al. 2015

Clinica Chimica Acta

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“…To date, the few studies published 23,24 that have already used this type of cytometers did not define the lower limit of analysis, impeding future comparisons. Thus, using a standardization strategy, we defined an accurate MP gate between 1 and 0.3 m bead-eq as the best compromise between a good FS resolution and a level of background noise that does not impede the cytometer performance.…”

Section: Discussionmentioning

confidence: 99%

High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles—Brief Report

Robert

Lacroix²,

Poncelet³

et al. 2012

ATVB

141

137

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Objective-Cellular microparticles (MP) are promising biomarkers in many pathological situations. Although flow cytometry (FCM) is widely used for their measurement, it has raised controversies because the smallest MP size falls below the detection limit of standard FCM (sd-FCM). Following recent technological improvements leading to high sensitivity FCM (hs-FCM), our objectives were (1) to evaluate the potential of hs-FCM for extended MP detection, (2) to set up a standardized protocol for MP enumeration, and (3) to compare MP counts obtained with both sensitivity levels. Methods and Results-Compared with sd-FCM, hs-FCM displayed improved forward scatter resolution and lower background noise, allowing us to discriminate previously undetectable small MP in plasma samples. Using fluorescent beads with appropriate sizes (0.1/0.3/0.5/0.9 m) and relative amounts, a new standardized hs-FCM MP protocol was set up and provided reproducible MP counts. Applied to coronary patient samples, it resulted into 8-to 20-fold increases in MP counts as compared with sd-FCM. Interestingly, the ratio between small and large MP varied according to clinical status but also depending on MP subset, suggesting access to new biological information. 3-5 Circulating MP are elevated in many prothrombotic and inflammatory disorders; cardiovascular, autoimmune, and infectious diseases; and cancer. 4,[6][7][8][9][10] Although MP counts may provide useful diagnostic/prognostic information, assessment of their pathophysiological relevance is hampered by methodological concerns. Flow cytometry (FCM) is the most commonly used technique, 11 allowing both enumeration and characterization of MP cellular origin with high throughput. The forward scatter (FS) parameter is generally used to define the analysis gate for MP. Unfortunately, because of their limited FS sensitivity, standard flow cytometers measure only a small part of the MP population. Recently, high-sensitivity flow cytometers with significantly improved light scatter detection became available. As we first reported, these instruments resolve a previously undetectable subpopulation of small-size MPs. Conclusion-Recent12 Among the technical improvements found in high sensitivity FCM (hs-FCM), a new option of the Beckman Coulter Gallios called W2 increases FS resolution by amplifying the FS signals collected at large angles. 13 Whether this option can be used in a standardized manner, to improve the detection of MPs derived from platelets (PMP), erythrocytes (Ery-MP), leukocytes (Leu-MP), and endothelial cells (EMP), remains an open question. Our objectives were then (1) to evaluate the impact of high-sensitivity flow cytometer technical improvement on the detection of small-size MP, (2) to set up a new standardized protocol based on calibrated microbeads for MP enumeration, and (3) to compare MP counts obtained at both sensitivity levels of FCM. MethodsBlood and plasma samples, MP preparation and flow cytometric analysis, size-conditioned MP filtration, intra-and inter-instrument reproducibi...

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“…+ ) (b) Endothelial-derived MPs (EMPs) levels (CD31 + /CD41 -). Plasma levels of EMPs and PMPs in 30 healthy controls subjects (12 ll AE 10 and 4515 ll AE 3787, respectively) were reported in a previous study (8), investigating the same psoriasis patients with similar MPs quantification method. Patients who did not respond to anti-TNF-a agents increased mean number of PMPs and EMPs (n = 6) between M0 and M3 (4065 ll vs 4617 ll and 26 ll vs 46 ll, respectively, not shown).…”

Section: Discussionmentioning

confidence: 99%

“…As psoriasis may also be associated with higher incidence of cardiovascular comorbidities (2-4), MPs may be involved in these complications through their procoagulant and atherosclerotic properties (5,6). Previous studies demonstrated that MPs issued from platelets (PMPs) and endothelial cells (EMPs) were significantly increased in psoriasis patients and correlated with the activity of psoriasis (7,8).…”

Section: Introductionmentioning

confidence: 99%

Effects of anti‐TNF‐α agents on circulating endothelial‐derived and platelet‐derived microparticles in psoriasis

Pelletier

Garnache‐Ottou

Biichlé

et al. 2014

Experimental Dermatology

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Psoriasis involves TNF-a secretion leading to release of microparticles into the bloodstream. We investigated the effect of TNF blockers on microparticles levels before and after treatment in patients (twenty treated by anti-TNF-a agents and 6 by methotrexate) with severe psoriasis. Plasmatic microparticles were labelled using fluorescent monoclonal antibodies and were analysed using cytometry. Three months later, 70% of patients treated with anti-TNF-a agents achieved a reduction in PASI score of at least 75%. The clinical improvement in patients treated with anti-TNF-a agents was associated with a significant reduction of the mean number of platelet microparticles (2837/ll vs 1849/ll, P = 0.02) and of endothelial microparticles (64/ll vs 22/ll, P = 0.001). Microparticles are significantly decreased in psoriatic patients successfully treated by anti-TNF-a. Microparticles levels as circulating endothelial cells represent signs of endothelial dysfunction and are elevated in psoriasis. Then, TNF blockade may be effective to reduce cardiovascular risk through the reduction of circulating microparticles.

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Increased Levels of Circulating Endothelial-Derived Microparticles and Small-Size Platelet-Derived Microparticles in Psoriasis

Cited by 53 publications

References 13 publications

Is enhanced platelet activation the missing link leading to increased cardiovascular risk in psoriasis?

Is enhanced platelet activation the missing link leading to increased cardiovascular risk in psoriasis?

High-Sensitivity Flow Cytometry Provides Access to Standardized Measurement of Small-Size Microparticles—Brief Report

Effects of anti‐TNF‐α agents on circulating endothelial‐derived and platelet‐derived microparticles in psoriasis

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