Increased Ischemia-Reperfusion Blood Flow Impairs the Skeletal Muscle Contractile Function

Ikebe, Kenshiro; Kato, Teiji; Yamaga, Makio; Hirose, Jun; Tsuchida, Toru; Takagi, Katsumasa

doi:10.1006/jsre.2001.6134

Cited by 16 publications

(11 citation statements)

References 21 publications

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“…Furthermore, both techniques indicated an advantage of the tourniquet. The increased post-ischemic blood flow is consistent with other studies [22]. Since we occluded the artery as well as the vein in either experimental group, both models could have been susceptible to hemorrhagic infarction which then could also have implicated disturbances in measuring edema formation.…”

Section: Discussionsupporting

confidence: 90%

An optimized low-pressure tourniquet murine hind limb ischemia reperfusion model: Inducing acute ischemia reperfusion injury in C57BL/6 wild type mice

et al. 2019

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Acute ischemia reperfusion injury in skeletal muscle remains an important issue in several fields of regenerative medicine. Thus, a valid model is essential to gain deeper insights into pathophysiological relations and evaluate possible treatment options. While the vascular anatomy of mice regularly prevents sufficient vessel occlusion by invasive methods, there is a multitude of existing models to induce ischemia reperfusion injury without surgical procedures. Since there is no consensus on which model to prefer, this study aims to develop and evaluate a novel, optimized low-pressure tourniquet model. C57BL/6 mice underwent an ischemic procedure by either tourniquet or invasive artery clamping. A sham group served as control. With exception of the sham group, mice underwent 2 hours of ischemia followed by 4 hours of reperfusion. Groups were compared using microcirculatory and spectroscopic measurements, distinctions in tissue edema, histological and immunohistochemical analyses. Both procedures led to a significant decrease in tissue blood flow (- 97% vs. - 86%) and oxygenation (- 87% vs. - 75%) with a superiority of the low-pressure tourniquet. Tissue edema in the tourniquet cohort was significantly increased (+ 59%), while the increase in the clamping cohort was non-significant (+ 7%). Haematoxylin Eosin staining showed significantly more impaired muscle fibers in the tourniquet group (+ 77 p.p. vs. + 11 p.p.) and increased neutrophil infiltration/ROI (+ 51 vs. + 8). Immunofluorescence demonstrated an equal increase of p38 in both groups (7-fold vs. 8-fold), while the increase in apoptotic markers (Caspase-3, 3-Nitrotyrosine, 4-Hydroxynonenal) was significantly higher in the tourniquet group. The low-pressure tourniquet has been proven to produce reproducible and thus reliable ischemia reperfusion injury. In addition, significantly less force was needed than previously stated. It is therefore an important instrument for studying the pathophysiology of ischemia reperfusion injury and for the development of prophylactic as well as therapeutic interventions.

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Section: Discussionsupporting

confidence: 90%

An optimized low-pressure tourniquet murine hind limb ischemia reperfusion model: Inducing acute ischemia reperfusion injury in C57BL/6 wild type mice

et al. 2019

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“…Ikebe et al investigated the relationship between the duration of ischaemia and the subsequent reperfusion blood flow in rats (18). They found an increased reperfusion blood flow after 90 minutes and 3 hours of ischaemia, but after 6 hours of ischaemia there was no significant increase in postischaemic blood flow.…”

Section: Discussionmentioning

confidence: 99%

“…This is called the ‘no‐reflow phenomenon’ and is probably caused by cellular swelling, thrombosis and white cell plugging in capillaries, which increases the resistance in the microcirculation (19). The incidence of no flow depends on the severity of both the ischaemic insult and the subsequent reperfusion injury (18). During reperfusion oxygen is provided to the tissues, which combines with the free radical species generated during ischaemia to form reactive oxygen species (ROS) (13).…”

Section: Discussionmentioning

confidence: 99%

Pattern of presentation of pressure ulcers in traumatic spinal cord injured patients in University College Hospital, Ibadan

Iyun

Malomo

Oluwatosin

et al. 2011

International Wound Journal

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The neurosurgery division in University College Hospital (U.C.H.) admits approximately one traumatic spinal cord injured (SCI) patient per week, most of whom stay a minimum of 42 days on admission. A common complication in these patients is the development of pressure ulcers, which contributes to a longer hospital stay and increased hospital expenses. The purpose of this study was to investigate the pattern of presentation of pressure ulcers in patients on admission and to propose policies or protocols to reduce the incidence. It is a prospective study of traumatic SCI patients managed on the neurosurgery ward from January 2003 to June 2004. The data was analysed using descriptive statistics. Sixty-seven patients were studied. The average hospital stay was 73 days. Thirteen (20%) of the patients were admitted with pressure ulcers, 32 (47·7%) developed it after admission. As much as 87·5% of pressure ulcers seen in the course of this study which occurred on admission in U.C.H. was in the first week of admission, 6·25% in the second week and the remaining 6·25% in the third week. Pressure ulcers were distributed as follows; 69% (42) in the sacral region, 18% (11) trochanteric, 5% (3) scalp, 1·5% (1) ankle, 1·5% (1) ischial tuberosity, the remaining 5% in other sites. Preventive measures for pressure ulcers consisted of basic skin care, pressure dispersion using fenestrated foams and alternating weight-bearing sites by regular turning. Pressure ulcers are commonest in the sacral and gluteal regions and tend to occur within the first week of admission in the neurosurgical wards.

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“…Trauma is an important cause of an acutely ischemic limb, and the potential for limb salvage in this situation is greatly improved with modern methods of vascular surgery, fracture fixation, and soft tissue reconstruction. The muscle injury is characterized by permeability edema, impaired muscle function, and muscle necrosis (Ikebe et al 2001). The associated systemic inflammatory response, of which lung is the most pertinent manifestation, represents the complex interplay between cytokine production and proinflammatory mediators-resulting in neutrophil recruitment and activation (Lechin andVaron 1994, Botha et al 1995).…”

mentioning

confidence: 98%

Hypertonic saline reduces skeletal muscle injury and associated remote organ injury following ischemia reperfusion injury

Dillon¹,

Laing²,

Chandler³

et al. 2008

Acta Orthopaedica

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Increased Ischemia-Reperfusion Blood Flow Impairs the Skeletal Muscle Contractile Function

Cited by 16 publications

References 21 publications

An optimized low-pressure tourniquet murine hind limb ischemia reperfusion model: Inducing acute ischemia reperfusion injury in C57BL/6 wild type mice

An optimized low-pressure tourniquet murine hind limb ischemia reperfusion model: Inducing acute ischemia reperfusion injury in C57BL/6 wild type mice

Pattern of presentation of pressure ulcers in traumatic spinal cord injured patients in University College Hospital, Ibadan

Hypertonic saline reduces skeletal muscle injury and associated remote organ injury following ischemia reperfusion injury

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