Increased Iron and Ferritin Content of Sputum From Patients With Cystic Fibrosis or Chronic Bronchitis

Stites, Steve W.; Walters, B. A. J.; O’Brien-Ladner, Amy; Bailey, Kirstin; Wesselius, Lewis

doi:10.1378/chest.114.3.814

Cited by 90 publications

(76 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The media was modified to include 3 mg ml À 1 ferritin as an alternate iron source to more appropriately reflect the conditions of the CF lung (Stites et al, 1998;Reid et al, 2004;Ghio et al, 2012;Hare et al, 2012). Other modifications included the absence of bovine serum albumin and an increase to 20 mg ml À 1 porcine stomach mucin to better reflect our experience with CF sputum consistency.…”

Section: Inoculation Of Wincf Systemmentioning

confidence: 99%

A Winogradsky-based culture system shows an association between microbial fermentation and cystic fibrosis exacerbation

et al. 2014

View full text Add to dashboard Cite

There is a poor understanding of how the physiology of polymicrobial communities in cystic fibrosis (CF) lungs contributes to pulmonary exacerbations and lung function decline. In this study, a microbial culture system based on the principles of the Winogradsky column (WinCF system) was developed to study the physiology of CF microbes. The system used glass capillary tubes filled with artificial sputum medium to mimic a clogged airway bronchiole. Chemical indicators were added to observe microbial physiology within the tubes. Characterization of sputum samples from seven patients showed variation in pH, respiration, biofilm formation and gas production, indicating that the physiology of CF microbial communities varied among patients. Incubation of homogenized tissues from an explant CF lung mirrored responses of a Pseudomonas aeruginosa pure culture, supporting evidence that end-stage lungs are dominated by this pathogen. Longitudinal sputum samples taken through two exacerbation events in a single patient showed that a two-unit drop in pH and a 30% increase in gas production occurred in the tubes prior to exacerbation, which was reversed with antibiotic treatment. Microbial community profiles obtained through amplification and sequencing of the 16S rRNA gene showed that fermentative anaerobes became more abundant during exacerbation and were then reduced during treatment where P. aeruginosa became the dominant bacterium. Results from the WinCF experiments support the model where two functionally different CF microbial communities exist, the persistent Climax Community and the acute Attack Community. Fermentative anaerobes are hypothesized to be the core members of the Attack Community and production of acidic and gaseous products from fermentation may drive developing exacerbations. Treatment targeting the Attack Community may better resolve exacerbations and resulting lung damage.

show abstract

Section: Inoculation Of Wincf Systemmentioning

confidence: 99%

A Winogradsky-based culture system shows an association between microbial fermentation and cystic fibrosis exacerbation

et al. 2014

View full text Add to dashboard Cite

show abstract

“…A role for iron in stimulation of biofilm formation on epithelial cells is a clinically relevant finding because the ASL in the CF lung has an iron concentration 400-fold higher than in a non-CF lung (41)(42)(43). Indeed, it appears that P. aeruginosa biofilms seem to have even greater need for iron than planktonic bacteria (17).…”

Section: Discussionmentioning

confidence: 99%

Tobramycin and FDA-Approved Iron Chelators Eliminate Pseudomonas aeruginosa Biofilms on Cystic Fibrosis Cells

Moreau‐Marquis¹,

O’Toole²,

Stanton³

2009

Am J Respir Cell Mol Biol

190

170

View full text Add to dashboard Cite

“…Interestingly, it has been recently reported that biofilm produced by strains of P. aeruginosa and B. cenocepacia is favoured by environmental stimuli, including oxygen deficiency and iron availability (3,18). With regard to iron, the concentration of iron and Lf in damaged CF respiratory tissues is high if compared to that of healthy controls (4)(5)19). The increase of iron availability favours the establishment of infections and damaging of airway tissues, while the increase ofLfis due to degranulation ofneutrophils recruited in infected and inflamed sites (5).…”

Section: Discussionmentioning

confidence: 99%

“…The ability to grow as biofilm is considered an important virulence factor used by pathogens to firmly colonize host tissues, for the development of infections and to resist the action of the host immune response and of antibiotics. It is conceivable that the high iron availability present in damaged airway tissues and exudates of CF patients (4)(5) might influence the in vivo formation of bacterial aggregates, biofilm and thus bacterial colonization. Therefore, iron availability might be an important signal recognized by P. aeruginosa and B. cenocepacia to influence their motile freeliving style and to enter into a new life-style, i.e.…”

mentioning

confidence: 99%

Bovine Lactoferrin Inhibits the Efficiency of Invasion of Respiratory A549 Cells of Different Iron-Regulated Morphological Forms of Pseudomonas Aeruginosa and Burkholderia Cenocepacia

Berlutti¹,

Superti

Nicoletti

et al. 2008

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

Pseudomonas aeruginosa and Burkholderia cenocepacia are two important opportunistic respiratory pathogens of cystic fibrosis (CF) patients. Infections caused by these microorganisms are particularly difficult to eradicate because they are usually highly resistant to several currently available broad-spectrum antibiotics. Lactoferrin (Lf), a glycoprotein found in physiological fluids of mammals and present at high concentrations in infected and inflamed tissues, plays an important role in the natural defence mechanism against pathogens and in immune regulation. In the present study, we evaluate the ability of bovine lactoferrin (bLf) to influence P. aeruginosa PAO1 and B. cenocepacia PV1 adhesiveness and invasiveness, using the A549 human bronchial cell line. Three different iron-induced morphological forms of bacteria (free-living, aggregates and biofilm) were assayed. The addition of bLf to cells just before infection had little influence on adhesion efficiency for all three of the morphological forms of B. cenocepacia PV1, while a slight increase in adhesion efficiency by P. aeruginosa PAO1 was noticed. Conversely, invasion of all three morphological forms of both P. aeruginosa and B. cenocepacia was strongly inhibited by the presence of bLf, independently of its degree of iron-binding activity. This is the first report demonstrating an anti-invasive property of bLf for strains of P. aeruginosa and B. cenocepacia.

show abstract

Increased Iron and Ferritin Content of Sputum From Patients With Cystic Fibrosis or Chronic Bronchitis

Cited by 90 publications

References 23 publications

A Winogradsky-based culture system shows an association between microbial fermentation and cystic fibrosis exacerbation

A Winogradsky-based culture system shows an association between microbial fermentation and cystic fibrosis exacerbation

Tobramycin and FDA-Approved Iron Chelators Eliminate Pseudomonas aeruginosa Biofilms on Cystic Fibrosis Cells

Bovine Lactoferrin Inhibits the Efficiency of Invasion of Respiratory A549 Cells of Different Iron-Regulated Morphological Forms of Pseudomonas Aeruginosa and Burkholderia Cenocepacia

Contact Info

Product

Resources

About