Increased invasion of ED-1 positive macrophages in both ipsi- and contralateral dorsal root ganglia following unilateral nerve injuries

Dubový, Petr; Tučková, Lucie; Jančálek, Radim; Svíženská, Ivana Hradilová; Klusáková, Ilona

doi:10.1016/j.neulet.2007.09.012

Cited by 50 publications

(48 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings implicate that CXCL12/CXCR4 axis might contribute to the pathogenesis of these complications of peripheral neuropathy. Previously, it was found that unilateral nerve injury excited neurons, activated satellite glia, and initiated macrophage invasion at both ipsilateral and contralateral DRG (Dubový et al, 2006(Dubový et al, , 2007, but the mechanisms underlying these phenomena remain unclear. Recently, it was shown that chronic constriction injury (CCI) operation induced chronic pain, which was associated with the increased expression of CXCR4 in neuron and satellite glia in bilateral lumbar and cervical DRG (Dubovy et al, 2010).…”

Section: Cxcl12/cxcr4 Axis and Sciatic Nerve Injurymentioning

confidence: 99%

CXCL12/CXCR4 axis: an emerging neuromodulator in pathological pain

Luo¹,

Wang²,

Xia³

et al. 2016

Reviews in the Neurosciences

View full text Add to dashboard Cite

AbstractThe roles of chemokine C-X-C motif ligand 12 (CXCL12) and its receptor chemokine C-X-C motif receptor 4 (CXCR4) reveal this chemokine axis as an emerging neuromodulator in the nervous system. In the peripheral and central nervous systems, both CXCL12 and CXCR4 are expressed in various kinds of nociceptive structures, and CXCL12/CXCR4 axis possesses pronociceptive property. Recent studies have demonstrated its critical roles in the development and maintenance of pathological pain, and both neuronal and glial mechanisms are involved in this CXCL12/CXCR4 axis-mediated pain processing. In this review, we summarize the recent development of the roles and mechanisms of CXCL12/CXCR4 axis in the pathogenesis of chronic pain by sciatic nerve injury, human immunodeficiency virus-associated sensory neuropathy, diabetic neuropathy, spinal cord injury, bone cancer, opioid tolerance, or opioid-induced hyperalgesia. The potential targeting of CXCL12/CXCR4 axis as an effective and broad-spectrum pharmacological approach for chronic pain therapy was also discussed.

show abstract

Section: Cxcl12/cxcr4 Axis and Sciatic Nerve Injurymentioning

confidence: 99%

CXCL12/CXCR4 axis: an emerging neuromodulator in pathological pain

Luo¹,

Wang²,

Xia³

et al. 2016

Reviews in the Neurosciences

View full text Add to dashboard Cite

show abstract

“…Following nerve injury there is an invasion of immune cells into the nervous system that is promoted largely by the Wallerian degeneration of the injured nerve as well as by damage of the tissue surrounding the nerve (Chung et al, 2007;Dubovy et al, 2007;Hu and McLachlan, 2002). The immune cells alter the neuronal environment in part by changing the level of various cytokines/chemokines produced by the immune cells and by the neurons and glial cells in response to those released by the immune cells (Austin and MoalemTaylor; Brazda et al, 2009;Dubovy et al, 2006;Dubovy et al, 2010a;Dubovy et al, 2010b).…”

Section: Peripheral Nerve Injury Alters the Dorsal Root Ganglia Envirmentioning

confidence: 99%

An Approach to Identify Nerve Injury-Evoked Changes that Contribute to the Development or Protect Against the Development of Sustained Neuropathic Pain

Recio-Pinto¹,

Norcini²,

Blanck³

2012

Basic Principles of Peripheral Nerve Disorders

View full text Add to dashboard Cite

“…Peripheral nerve injury induces many cellular and molecular changes in the associated DRG, including invasion of blood-derived cells (Dubovy et al 2007;Hu and McLachlan 2003), which activity could be important in initiating neuropathic pain (Bennett 2000;Moalem and Fig. 6 a Behavioral tests illustrating mechanical allodynia and thermal hyperalgesia of the hind paws following unilateral CCI of the sciatic nerve (n = 6) and AMD3100 administration at days 5 and 7 from operation.…”

Section: Sdf1/cxcr4 Immunostaining and Chemotactic Evectmentioning

confidence: 99%

“…Up-regulation of chemokines and their receptors in the DRG is suggested to be a way of local signaling for recruitment of macrophages and other bloodderived cells (Morin et al 2007;Tanaka et al 2004;White et al 2005). Bilateral up-regulation of some chemokines following unilateral nerve injury (White et al 2005) may contribute to the bilateral macrophage invasion into DRG (Dubovy et al 2007). However, our results from immunohistochemical detection of SDF1 protein and its CXCR4 receptor in the DRG following unilateral CCI may not suggest an involvement of SDF1/CXCR4 in the stimulation of blood-borne cell invasions.…”

Section: Sdf1/cxcr4 Immunostaining and Chemotactic Evectmentioning

confidence: 99%

“…Moreover, there is a substantial body of literature indicating extensive neuroinXammatory reactions to nerve injury that manifest themselves in behaviors suggestive of neuropathic pain. For example, immune cell inWltration at the nerve injury site (Stoll and Muller 1999) and DRG (Dubovy et al 2007;Hu and McLachlan 2002) as well as increased levels of proinXammatory cytokines (De Jongh et al 2003;Sommer and Kress 2004;Uceyler et al 2007) have been demonstrated in models of neuropathic pain.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spatio-temporal changes of SDF1 and its CXCR4 receptor in the dorsal root ganglia following unilateral sciatic nerve injury as a model of neuropathic pain

Dubový

Klusáková

Svíženská

et al. 2010

Histochem Cell Biol

Self Cite

View full text Add to dashboard Cite

There is a growing evidence that chemokines and their receptors play a role in inducing and maintaining neuropathic pain. In the present study, unilateral chronic constriction injury (CCI) of rat sciatic nerve under aseptic conditions was used to investigate changes for stromal derived factor-1 (SDF1) and its CXCR4 receptor in lumbal (L4-L5) and cervical (C7-C8) dorsal root ganglia (DRG) from both sides of naïve, CCI-operated and sham-operated rats. All CCI-operated rats displayed mechanical allodynia and thermal hyperalgesia in hind paws ipsilateral to CCI, but forepaws exhibited only temporal changes of sensitivity not correlated with alterations in SDF1 and CXCR4 proteins. Naïve DRG displayed immunofluorescence for SDF1 (SDF1-IF) in the satellite glial cells (SGC) and CXCR4-IF in the neuronal bodies with highest intensity in small- and medium-sized neurons. Immunofluorescence staining and Western blot analysis confirmed that unilateral CCI induced bilateral alterations of SDF1 and CXCR4 proteins in both L4-L5 and C7-C8 DRG. Only lumbal DRG were invaded by ED-1+ macrophages exhibiting SDF1-IF while elevation of CXCR4-IF was found in DRG neurons and SGC but not in ED-1+ macrophages. No attenuation of mechanical allodynia, but reversed thermal hyperalgesia, in ipsi- and contralateral hind paws was found in CCI-operated rats after i.p. administration of CXCR4 antagonist (AMD3100). These results indicate that SDF1/CXCR4 changes are not limited to DRG associated with injured nerve but that they also spread to DRG non-associated with such nerve. Functional involvement of these alterations in DRG non-associated with injured nerve in neuropathic pain remains to be elucidated.

show abstract

Increased invasion of ED-1 positive macrophages in both ipsi- and contralateral dorsal root ganglia following unilateral nerve injuries

Cited by 50 publications

References 30 publications

CXCL12/CXCR4 axis: an emerging neuromodulator in pathological pain

CXCL12/CXCR4 axis: an emerging neuromodulator in pathological pain

An Approach to Identify Nerve Injury-Evoked Changes that Contribute to the Development or Protect Against the Development of Sustained Neuropathic Pain

Spatio-temporal changes of SDF1 and its CXCR4 receptor in the dorsal root ganglia following unilateral sciatic nerve injury as a model of neuropathic pain

Contact Info

Product

Resources

About