Increased Intraabdominal Pressure Impairs Liver Regeneration after Partial Hepatectomy in Rats

Kaya, Yavuz; Aral, Erinc; Coşkun, Teoman; Erkasap, Nilüfer; Var, Ahmet

doi:10.1006/jsre.2002.6555

Cited by 10 publications

(15 citation statements)

References 27 publications

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“…nude mice or in nude mice subjected to PH. However, in mice suffering from liver injury 24 h after Jo2 or TA In the present study, 70% PH, a major surgical trauma leading to an inflammatory response and edema treatment, the engraftment of 2n hepatocytes was about twice that of an unpurified hepatocyte population or of in the remnant liver (29), was lethal for all nude mice, while as previously reported (4) and as also found in the a population enriched in 4n hepatocytes. present study their counterparts Balb/c much more DISCUSSION readily survived this trauma.…”

Section: Hepatocyte Transplantation and Immunodetectionsupporting

confidence: 89%

“…No mice stained sections revealed onset of necrosis involving the centrolobular and mid-zonal areas 24 h after 1700 mg/ died between day 3 and day 10. As Kaya et al (29) linked augmentation of intra-abdominal pressure to imkg TA injection to nude mice. The cocardious aspect of necrosis, as well as a significant increase in serum ALT pairment of liver regeneration after PH, one may hypothesize that the increased mortality was due to incontent, suggested fulminant hepatitis (Fig.…”

Section: After Partial Hepatectomymentioning

confidence: 99%

See 1 more Smart Citation

Experimental Models of Acute and Chronic Liver Failure in Nude Mice to Study Hepatocyte Transplantation

Gandillet¹,

Vidal

Alexandre³

et al. 2005

Cell Transplant

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Although hepatocyte transplantation is a promising therapy for acute liver failure in human, there is still a lack of animal models suffering from hepatic injury in which the benefits of hepatocyte transplantation could be evaluated solely, without the bias caused by immunosuppression. As a consequence, the aim of the study was first to develop reproducible models of partial hepatectomy and of thioacetamide (TA)-or Jo2-induced acute liver failure in nude mice. Chronic liver disease was also investigated by repeated injections of sublethal doses of thioacetamide. Survival rates, routine histologic observations, alanin aminotransferase sera content, Ki67, and caspase 3 immunodetection were investigated both after 40% partial hepatectomy and after toxic-induced damages. Liver injuries were more severe and/or precocious in nude mice than in Balb/ c mice for a given treatment with a maximum of acute injury obtained 24 h after single toxic injection, and were found to be transitory and reversible within 10 days. Toxics induced apoptosis followed by necrosis, confirming recent published data. Onset of fibrosis leading to reproducible chronic cirrhosis in nude mice correlated with increasing number of Ki67-positive cells, indicating that high levels of cell proliferation occurred. Chronic cirrhosis progressively reversed to fibrosis when the treatment ceased. Preliminary results demonstrated that engrafted xenogeneic hepatocytes could be detected in the host liver by anti-MHC class I immunohistochemistry. Fractions enriched in 2n or 4n hepatocytes by cell sorting using a flow cytometer were equivalent to the unpurified fraction in terms of engraftment in control nude mice or in nude mice subjected to PH. However, in mice suffering from liver injury 24 h after Jo2 or TA treatment, the engraftment of 2n hepatocytes was about twice that of an unpurified hepatocyte population or of a population enriched in 4n hepatocytes.

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Section: Hepatocyte Transplantation and Immunodetectionsupporting

confidence: 89%

Section: After Partial Hepatectomymentioning

confidence: 99%

Experimental Models of Acute and Chronic Liver Failure in Nude Mice to Study Hepatocyte Transplantation

Gandillet¹,

Vidal

Alexandre³

et al. 2005

Cell Transplant

View full text Add to dashboard Cite

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“…In contrast to recent experimental studies, we observed the negative impact of a relatively low-pressure pneumoperitoneum on liver regeneration [30]. One cause for the observed adverse effect of the pneumoperitoneum on liver regeneration may be the ischemia/reperfusion injury with production of oxygen-derived free radicals and inflammatory cytokines contributing to organ dysfunction [36].…”

Section: Discussionmentioning

confidence: 50%

“…Two recently published experimental studies suggest that a pneumoperitoneum in association with partial hepatectomy leads to a reduction in postoperative liver regeneration [27,30]. Kaya et al [30] observed a significant reduction in liver regeneration on days 1 and 4 after partial hepatectomy followed by a long-term pneumoperitoneum of 12-14 mmHg for 24 h. Yagmurdur et al [27] also showed an impairment of liver regeneration on POD 5 in rats subjected to a 15-mmHg pneumoperitoneum for 60 min followed by partial hepatectomy.…”

Section: Discussionmentioning

confidence: 99%

The impact of carbon dioxide pneumoperitoneum on liver regeneration after liver resection in a rat model

et al. 2009

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“…Total hepatic ischemia has been shown to produce several pathological events, including splanchnic congestion, severe intestinal ischemia, mesenteric congestion, and systemic shock, thereby resulting in a high mortality rate (21,22,32,37). Furthermore, occlusion to all lobes of the liver causes venous congestion in the mesenteric bed and compromises the intestinal mucosa, resulting in bacterial translocation and onset of systemic inflammatory response syndrome (4,25).…”

Section: Technique Of Portal Triad Occlusionmentioning

confidence: 99%

Use of a hanging-weight system for liver ischemic preconditioning in mice

Hart

Much²,

Köhler³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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Hart ML, Much C, Kö hler D, Schittenhelm J, Gorzolla IC, Stahl GL, Eltzschig HK. Use of a hanging-weight system for liver ischemic preconditioning in mice. Am J Physiol Gastrointest Liver Physiol 294: G1431-G1440, 2008. First published April 24, 2008 doi:10.1152/ajpgi.00083.2008 represents a powerful experimental strategy to identify novel molecular targets to attenuate hepatic injury during ischemia. As a result, murine studies of hepatic IP have become an important field of research. However, murine IP is technically challenging, and experimental details can alter the results. Therefore, we systematically tested a novel model of hepatic IP by using a hanging-weight system for portal triad occlusion. This system has the benefit of applying intermittent hepatic ischemia and reperfusion without manipulation of a surgical clamp or suture, thus minimizing surgical trauma. Systematic evaluation of this model revealed a close correlation of hepatic ischemia time with liver damage as measured by alanine (ALT) and aspartate (AST) aminotransferase serum levels. Using different numbers of IP cycles and times intervals, we found optimal liver protection with four cycles of 3 min ischemia/3 min reperfusion as measured by ALT, AST, lactate dehydrogenase, and interleukin-6. Similarly, ischemiaassociated increases in hepatic infarct size, neutrophil infiltration, and histological injury were maximally attenuated with the above regimen. To demonstrate transcriptional consequences of liver IP, we isolated RNA from preconditioned liver and confirmed transcriptional modulation of known target genes (equilibrative nucleoside transporters, acute-phase complement genes). Taken together, these studies confirm highly reproducible liver injury and protection by IP when using the hanging-weight system for hepatic ischemia and intermittent reperfusion. Further studies of murine IP may consider this technique.

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Increased Intraabdominal Pressure Impairs Liver Regeneration after Partial Hepatectomy in Rats

Cited by 10 publications

References 27 publications

Experimental Models of Acute and Chronic Liver Failure in Nude Mice to Study Hepatocyte Transplantation

Experimental Models of Acute and Chronic Liver Failure in Nude Mice to Study Hepatocyte Transplantation

The impact of carbon dioxide pneumoperitoneum on liver regeneration after liver resection in a rat model

Use of a hanging-weight system for liver ischemic preconditioning in mice

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