Increased interleukin 1α and interleukin 1β expression is involved in the progression of periapical lesions in primary teeth

Abstract

BackgroundInterleukin 1 (IL-1) is involved in bone resorption. However, the role of IL-1 in periapical lesions characterized by periapical bone destruction in primary teeth has not yet been fully elucidated. This study aimed to detect the distribution and expression of IL-1 in periapical lesions in primary teeth and assess the relationship between the cytokines and the degree of inflammatory cell infiltration.MethodsA total of 106 chronic periapical lesions in primary teeth were harvested. Haematoxylin and eosin (H&E) staining was used to determine the histological type and the inflammatory cell infiltration grade (mild, moderate, and severe), and immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the distribution and expression of IL-1α and IL-1β.ResultsOf the 106 chronic periapical lesion samples, there were 85 cases of periapical granuloma, accounting for 80.19% of the total samples, and 21 cases of radicular cysts, accounting for 19.81%; no cases of abscess were detected. Immunohistochemistry results showed that both IL-1α and IL-1β were expressed in periapical granulomas and cysts. ELISA results showed that IL-1α and IL-1β levels were higher in the periapical granuloma group than in the radicular cyst and normal control groups (P < 0.05). In the periapical granuloma group, IL-1α and IL-1β were detected at higher levels in the severe inflammatory cell infiltration subgroup than in the mild-inflammatory cell infiltration subgroup (P < 0.05), and IL-1β expression was also higher in the moderate inflammatory cell infiltration subgroup than in the mild inflammatory cell infiltration subgroup (P < 0.01). A significant positive correlation was observed between the protein expression levels of IL-1α and IL-1β and the inflammation grade in periapical granulomas from primary teeth (P < 0.05).ConclusionExpression levels of the cytokines IL-1α and IL-1β in periapical granulomas from primary teeth increased with increasing inflammatory severity and appeared to be a contributing factor to the progression of periapical lesions.

Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems

“…persistence of the lesion in this study may be correlated with the non-modulation of this cytokine by endodontic treatment. In apical periodontitis of deciduous human teeth, interleukin-1α and interleukin-1β were expressed by different cell types in granulomas and root cysts, such as inflammatory cells, epithelial cells and vascular endothelium (36).…”

Tratamento endodôntico em sessão única utilizando diferentes sistemas de irrigação de canais radiculares: avaliação radiográfica e imunohistoquímica

“…Uma vez que a citocina pró-inflamatória interleucina-1 é um reguladorchave das respostas do hospedeiro à infecção microbiana, aumentando a reabsorção e inibindo a formação óssea (Ng et al, 2008), a persistência da lesão neste estudo pode estar correlacionada a não modulação dessa citocina pelo tratamento endodôntico. Em lesões periapicais de dentes decíduos de humanos, IL-1α e IL-1β foram expressas por diferentes tipos celulares em granulomas e cistos radiculares, tais como células inflamatórias, células epiteliais e no endotélio vascular (Yang et al, 2018).…”

Avaliação da eficácia anestésica da Mepivacaína na exodontia de molares inferiores com lesão apical

“…Trabalho de Stashenko et al,1994, com (Martinez et al, 2007;Yang et al, 2018), sendo a IL-1β predominante em lesões periapicais humanas (Miller et al, 1996).…”

“…Muitos são os trabalhos que referenciam a função dos neuropeptídeos, mediadores inflamatórios, células imunológicas, citocinas, interleucinas e ramificação de nervosas como contribuidores para o desenvolvimento e manutenção da lesão apical (Hargreaves et al, 1994;Miller et al, 1996;Liapatas et al, 2003;Båge et al, 2011;Bracks et al, 2014;Austah et al, 2016 ;Martinho et al, 2017;Mehrazarin et al, 2017, Francisconi et al, 2018. Muitos trabalhos contribuiram para elucidarar o papel dos mediadores, como Substância P (Kabashima et al, 2002), TNFα (Safavi, Rossomando, 1991;Pezelj-Ribarić et al,2007,) PGE2 Takayama et al, 1996,), IL-1α e IL-1β (Martinez et al, 2007;Zhang et al, 2016;Yang et al,2018), LTB4 (Torabinejad et al, 1992)…”

“…O granuloma, ou lesão apical, é histologicamente, um infiltrado inflamatório basicamente formado por macrófagos, linfócitos, leucócitos e neutrófilos (Ishimi et al,1990;Safavi, Ressomando 1991;Wajant et al,1998, Boyce et al,2005Jurisic et al, 2008, Omoregie et al, 2011Jakovljevic et al,2015;Berar et al, 2016,). Nesse tipo de lesão, há acúmulo de IL-1α e IL-1β (Martinez et al, 2007, Yang et al,2018, sendo a IL-1β predominante em lesões periapicais humanas (Miller et al, 1996). Parece haver correlação positiva entre os níveis de IL-1, IL-2, IL-6 e sintomas de dor (Shimauchi et al,2001, bem como da presença de substância P Austah et al,2016), de prostaglandinas (Offenbacher et al, 1993;Takayama et al, 1996, Båge et al, 2011Shahriari et al, 2011) e do fator de necrose tumoral (TNF) (Safavi, Ressomando, 1991, Artese et al, 1991, Wajant 1998Kabashima et al, 2002;Boyce et al, 2005;Pezelj-Ribaric et al, 2007;Jurisic et al, 2008Shahriari et al, 2011, pois são indutores de hiperalgesia pela produção de IL-1β e pela quimiotaxia de neutrófilos (Shimauchi etal, 2001,Verri et al, 2006, McAller et al, 2007, Gold, Flake, 2005.…”