Increased incidence of micronuclei assessed with the micronucleus assay and the fluorescence in situ hybridization (FISH) technique in peripheral blood lymphocytes of nurses exposed to nitrous oxide

Lewińska, Dobrosława; Stępnik, Maciej; Krajewski, Wojciech; Arkusz, Joanna; Stańczyk, Małgorzata; Wrońska-Nofer, T

doi:10.1016/j.mrgentox.2004.10.018

Cited by 28 publications

(16 citation statements)

References 31 publications

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“…In the present study, we observed a significant increase in MN frequency in the exposed group, in both genders. There are previous reports in the literature showing a significant increase in MN frequency in exposed women [31,37,43]. In our study, however, we also found a difference in the man professionals.…”

Section: Discussioncontrasting

confidence: 54%

“…Neurotoxic [32], hepatotoxic [22], nephrotoxic [24,32] and carcinogenic effects [13,32,39], as well as fertility alterations, increased incidence of spontaneous abortions and congenital abnormalities have been observed in these professionals [3,24,41,45,46]. Nevertheless, there is still some controversy regarding the genotoxic and/or mutagenic effects of acute or chronic exposure to anesthetic gases [14,25,27], even though significant increases in the frequency of chromosome aberrations and of micronuclei (MN) in peripheral blood lymphocytes of professionals exposed to such agents have been reported [8,11,12,29,31,35,42,44].…”

Section: Introductionmentioning

confidence: 99%

“…The micronucleus test is a method for the assessment of several kinds of cytogenetic damages, used in the biomonitoring of human populations for evaluating the risk of developing cancer and identifying the genotoxic potential of chemical, physical and biological agents [1,31,35,42].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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Exposure to certain chemical agents causes damage to the genetic material. There is controversy about the genotoxic and/or mutagenic effect caused by acute or chronic anesthetics exposure. The aim of this research was to assess and compare the frequency of micronuclei (MN) in professionals working in operating rooms and other hospital areas. The genotoxic and cytotoxic effects were evaluated in lymphocytes using the micronucleus test and the proliferative index, respectively. We examined peripheral blood lymphocyte cultures from 30 operating room professionals (exposed group) and 30 professionals non-exposed in other hospital areas of the same hospital (control group). There was no statistically significant difference in proliferative index between the groups. Nevertheless, there was an increase in MN frequency in binucleated (BN) cells in the exposed group (p=0.0003) compared to the control group. Moreover, there was a statistically significant difference between genders (p=0.0187), and the frequency of MN in BN cells from women was higher than in men. Therefore, gender influenced the frequency of MN. The age and period of working time in an operating room influenced the MN frequency only in women professionals. Thereby, there was a genotoxic effect in occupationally exposed professionals, and the micronucleus test could be used in the biomonitoring of human populations for evaluating the risk of developing cancer.

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Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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“…They concluded that long-term verapamil therapy could potentially increase the effects of certain environmental mutagens. Earlier published literature data on genotoxic effects of anaesthetic gases in humans after chronic exposure show significant increases in the frequency of MN in peripheral blood lymphocytes [19]. Klimova and Paskevich [20] studied in vivo and in vitro effects of barbiturate anaesthetising substances (hexenal and sodium thiopental) on the frequency of chromosomal aberrations in human peripheral blood leukocytes, and they concluded that the frequency of aberrations in vivo was at the level of the spontaneous mutation after the use of sodium thiopental (pentotal).…”

Section: Discussionmentioning

confidence: 90%

The effect of combined therapy with ritodrine, erythromycin and verapamil on the frequency of micronuclei in peripheral blood lymphocytes of pregnant women

et al. 2007

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The main aim of this study was to investigate the genotoxic effect of combined pharmacotherapy applied in post-operative treatment of cervical cerclage in pregnant women over six days. This study included 19 phenotypically healthy pregnant women in mid-trimester with a diagnosis of cervical insufficiency, mean age 28+/-5.33. The frequency of micronuclei (MN) was estimated in peripheral blood lymphocytes of patients before surgical intervention and after the end of applied pharmacotherapy by application of cytokinesis block micronucleus (CBMN) test. Mean value of baseline MN frequency was 6.84+/-2.91 MN/1000 binucleated cells, and after the end of the applied therapy, 10.32+/-4.27 MN/1000 binucleated cells (P<0.001) were found. The data of cell proliferation index showed that the combined therapy did not induce significant difference in cell kinetics (P>0.05). Our results showed that combined pharmacotherapy treatment over six days significantly increased the frequency of MN in peripheral blood lymphocytes of pregnant women.

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“…2,3,[17][18][19] Similar findings have also been demonstrated with MN test in occupationally exposed anesthesiology personnel. [17][18][19][20][21][22] Wiesner et al 23 found a higher MN level in anesthesiologists and nurses exposed to high level of halothane and isoflurane compared with the control group. Findings of SCE test in anesthesiology personnel are inconsistent.…”

Section: Commentmentioning

confidence: 99%

DNA Damage, Glutathione, and Total Antioxidant Capacity in Anesthesia Nurses

İzdeş

Şardaş

Kadıoğlu

et al. 2010

Archives of Environmental & Occupational Health

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The possibilities of a potential mutagenic/carcinogenic action of waste anesthetic gases in occupationally exposed anesthesia personnel have been previously reported in several studies. The aim of this study was to assess the DNA damage, reduced glutathione (GSH), and total antioxidant capacity (TAC) in anesthesia nurses. DNA damage was determined with comet assay, GSH levels were measured spectrophotometrically, and TAC was determined by using Randox kit. Anesthesia nurses (n = 40) showed increased DNA damage in terms of mean percentage of the total DNA in the comet tail compared to controls (n = 40) (p < .001). Mean TAC and GSH levels of the anesthesia nurses were significantly lower than that of the controls (p < .001, p < .05, respectively). The results of this study indicate that occupational exposure to anesthetic gases induce DNA damage, which may lead to changes in TAC and GSH levels.

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Increased incidence of micronuclei assessed with the micronucleus assay and the fluorescence in situ hybridization (FISH) technique in peripheral blood lymphocytes of nurses exposed to nitrous oxide

Cited by 28 publications

References 31 publications

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

The effect of combined therapy with ritodrine, erythromycin and verapamil on the frequency of micronuclei in peripheral blood lymphocytes of pregnant women

DNA Damage, Glutathione, and Total Antioxidant Capacity in Anesthesia Nurses

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