Increased immature hematopoietic progenitor cells CD34⁺/CD38^dim in myelodysplasia

Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group

“…27,28 A decrease in CD38 expression on CD34 þ myeloid progenitor cells has also been reported as a rather typical aberrancy in MDS. 29,30 Analysis of the maturing myeloid and monocytic compartment Definition of neutrophils. The combination of CD45 and SSC is regularly applied to identify maturing neutrophils by FC (CD45 int SSC int-bright ).…”

Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes

“…Several studies have used flow cytometry to try and distinguish low-risk MDS from non-clonal cytopenias by assessing the pattern of myeloid/erythroid/lymphoid maturation from progenitors and the immunophenotype of the CD34 + compartment. [3][4][5][6][7][8][9][10][11][12][13] However, no one simple flow-based marker reliably differentiates MDS from non-clonal cytopenias. This has given rise to a number of scoring systems combining multiple measurements.…”

“…8 Thus, we investigated whether reduced mean fluorescence intensity (MFI) of CD38 expression on CD34 + cells could be used as a surrogate marker for abnormalities in the MDS CD34 + compartment and whether this would provide a simple useful single flow cytometric measurement diagnostic of MDS. Figure S1).…”

Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease: A pilot study on a series of 56 patients

“…In parallel, a number of reagent combinations have been proposed that generate unique immunophenotypic patterns of antigen expression for both maturing neutrophils and erythroid precursors, which are frequently altered in MDS (13,(16)(17)(18)(19)(20). However, detailed analysis of these studies shows that they have typically focused on the study of BM CD34 þ hematopoietic progenitor cells (HPC) (21)(22)(23)(24)(25) and/or a few major compartments of maturing neutrophil, monocytic and to lower extent, also erythroid and megakaryocytic cells (19)(20)(21)(22)(23)(24)(25)(26). In addition, although in few of these reports immunophenotypic scores (IS) have been built according to the number of abnormalities identified and their nature (13,19,23), such scores are frequently based on relatively subjective criteria (13) and/or the analysis of a restricted number of BM cell populations (15,23,27).…”