Increased IL-17 expression is associated with pathology in a bovine model of tuberculosis

Blanco, Federico Carlos; Bianco, María Verónica; Meikle, Virginia; Garbaccio, Sergio; Vagnoni, Lucas; Forrellad, Marina Andrea; Klepp, Laura I.; Cataldi, Angel; Bigi, Fabiana

doi:10.1016/j.tube.2010.11.007

Cited by 47 publications

(39 citation statements)

References 41 publications

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“…The expression of IL-17, an inflammatory cytokine involved in granulopoiesis and neutrophil recruitment, is significantly induced in response to infection with Mycobacterium (36,37,65,72). In cattle, there were reports (66,73) of IL-17 mRNA expression following infection with M. bovis; however, to our knowledge, ours is the first confirmation that lymphocytes from M. bovisinfected animals produce IL-17A protein (Fig. 3).…”

Section: Discussionmentioning

confidence: 57%

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

McGill

Sacco

Baldwin

et al. 2014

The Journal of Immunology

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Promoting effective immunity to Mycobacterium bovis infection is a challenge that is of interest to the fields of human and animal medicine alike. We report that γδ T cells from virulent M. bovis–infected cattle respond specifically and directly to complex, protein, and nonprotein mycobacterial Ags. Importantly, to our knowledge, we demonstrate for the first time that bovine γδ T cells specifically recognize peptide Ags derived from the mycobacterial protein complex ESAT6:CFP10 and that this recognition requires direct contact with APCs and signaling through the T cell Ag receptor but is independent of MHC class I or II. Furthermore, we show that M. bovis infection in cattle induces robust IL-17A protein responses. Interestingly, in contrast to results from mice, bovine CD4 T cells, and not γδ T cells, are the predominant source of this critical proinflammatory mediator. Bovine γδ T cells are divided into subsets based upon their expression of Workshop Cluster 1 (WC1), and we demonstrate that the M. bovis–specific γδ T cell response is composed of a heterogeneous mix of WC1-expressing populations, with the serologically defined WC1.1+ and WC1.2+ subsets responding in vitro to mycobacterial Ags and accumulating in the lesions of M. bovis–infected animals. The results described in this article enhance our understanding of γδ T cell biology and, because virulent M. bovis infection of cattle represents an excellent model of tuberculosis in humans, contribute to our overall understanding of the role of γδ T cells in the mycobacterial-specific immune response.

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Section: Discussionmentioning

confidence: 57%

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

McGill

Sacco

Baldwin

et al. 2014

The Journal of Immunology

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“…Significant IL-17 responses are elicited by M. tuberculosis infection of mice (42) and humans (43,44), as well as M. bovis infection of cattle (7,11). With M. tuberculosis infection of mice, early expression of IL-17 in response to vaccination is required for the rapid accumulation of protective memory cells in the lungs (45).…”

Section: Discussionmentioning

confidence: 99%

“…PPD-specific IL-17 mRNA responses at 60 and 90 days after experimental M. bovis infection correlated with the presence of gross tuberculous lesions at necropsy 4 months after challenge (7). Using laser capture microdissection followed by qPCR, Aranday-Cortes et al demonstrated increased IL-17A and IL-22 expression within tuberculous granulomas versus nonaffected tissues from experimentally infected cattle, particularly in more advanced lesions (50).…”

Section: Figmentioning

confidence: 99%

“…While TSTs are the mainstays for bovine TB antemortem testing, blood-based tests are attractive, as they require only a single visit to the farm, provide the opportunity for immediate repeat testing, and yield results that are less subjective than those of TSTs. Additional biomarkers have recently emerged as potential candidates for use in blood-based TB tests for humans (reviewed by Walzl et al [5] and Salgame et al [6]) and cattle, including interleukin-1␤ (IL-1␤), IL-2, tumor necrosis factor alpha (TNF-␣), nitric oxide, IL-17, and IFN-␥-induced protein 10 (IP-10) (7)(8)(9)(10)(11)(12)(13). Of these, IL-17 is particularly appealing as a biomarker for bovine TB, as IL-17A mRNA responses determined using real-time quantitative PCR (RT-qPCR) may be predictive of both vaccine efficacy (11,14,15) and lesion severity (7) when measured after vaccination and infection, respectively.…”

mentioning

confidence: 99%

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Interleukin-17A as a Biomarker for Bovine Tuberculosis

Waters

Maggioli

Palmer

et al. 2016

Clin Vaccine Immunol

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“…Knowledge about response elements and gene regulation have allowed researchers to make refinements to diagnostics and discover approaches to aid in bTB detection (10). The host response measures utilized for bTB diagnostics include an array of cytokines, chemokines, and antibody responses to M. bovis proteins (5,11). Although these tools provide another layer of testing, the results obtained are often confounded by crossreactivity due to homology between mycobacterial species (12).…”

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confidence: 99%

Circulating Mycobacterium bovis Peptides and Host Response Proteins as Biomarkers for Unambiguous Detection of Subclinical Infection

et al. 2014

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e Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating Mycobacterium bovis infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (PLoS One 4:e5478, 2009, doi:10.1371/journal.pone.0005478) identified 32 host peptides that specifically increased in the blood serum of M. bovis-infected animals). In the current study, 16 M. bovis proteins were discovered in the blood serum proteomics data sets. A large-scale validation analysis was undertaken for selected host and M. bovis proteins using a cattle serum repository containing M. bovis (n ‫؍‬ 128), Mycobacterium kansasii (n ‫؍‬ 10), and Mycobacterium avium subsp. paratuberculosis (n ‫؍‬ 10), cases exposed to M. bovis (n ‫؍‬ 424), and negative controls (n ‫؍‬ 38). Of the host biomarkers, vitamin D binding protein (VDBP) showed the greatest sensitivity and specificity for M. bovis detection. Circulating M. bovis proteins, specifically polyketide synthetase 5, detected M. bovis-infected cattle with little to no seroreactivity against M. kansasii-and M. avium subsp. paratuberculosis-infected animals. These data indicate that host and pathogen serum proteins can serve as reliable biomarkers for tracking M. bovis infection in animal populations.

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Increased IL-17 expression is associated with pathology in a bovine model of tuberculosis

Cited by 47 publications

References 41 publications

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

Specific Recognition of Mycobacterial Protein and Peptide Antigens by γδ T Cell Subsets following Infection with VirulentMycobacterium bovis

Interleukin-17A as a Biomarker for Bovine Tuberculosis

Circulating Mycobacterium bovis Peptides and Host Response Proteins as Biomarkers for Unambiguous Detection of Subclinical Infection

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