Increased IL-10 mRNA expression in tumor-associated macrophage correlated with late stage of lung cancer

Abstract

BackgroundMonocyte recruited into the tumor and maturation to tumor-associated macrophage (TAM). Interleukin-10(IL-10) is a potent immunosuppressive cytokine, which can be secreted from both primary tumor and stromal cells. However, there are controversies regarding its role in the progression of cancer. So it is important to isolate TAM from tumor cells to study the role of IL-10 in the progress of cancer. The aim of our study was to determine whether IL-10 expressed by TAM correlated with clinicopathological factors in NSCLC.MethodsTAM in NSCLC was isolated by short-term culture in serum free medium with the modification to literature reports. The mRNA expression levels of IL-10, cathepsin B, cathepsin S, which were closely related with TAM according to the literatures, were evaluated by Quantitative real-time RT-PCR in 63 NSCLC. The relationships between their expression levels and clinicopathological features were investigated.ResultsWe successfully achieved up to 95% purity of TAM, derived from 63 primary lung cancer tissues. TAM expressed high levels of IL-10, cathepsin B in NSCLC. High levels of IL-10 in TAM significantly correlated with stage, tumor size, lymph node metastasis, lymphovascular invasion or histologic poor differentiation.ConclusionsOur results revealed that TAM with high levels of IL-10 expression may play an important role in the progression of non-small cell lung cancer. The data also suggested that TAMs may involve in tumor immunosuppression through overexpressed IL-10. Additionally, the phenotype of isolated TAM can be potentially used to predict clinicopathological features as well.

Monocytic myeloid-derived suppressor cells as a potent suppressor of tumor immunity in non-small cell lung cancer

“…Furthermore, Xiu et al (40) demonstrated that IL-10 is responsible for the development of Mo-MDSCs in B-cell NHL. Elevated production of IL-10 by macrophages associated with the tumor microenvironment correlates with poor prognosis, stage of disease, tumor size and lymph node metastasis (38,41). This correlation was confirmed in patients with NSCLC (42,43).…”

IL-10 Promotes Tumor Aggressiveness via Upregulation of CIP2A Transcription in Lung Adenocarcinoma

“…IL-10 is considered to be an autocrine growth factor of immune cells, and it participates notably in increases of tumor cell proliferation of melanoma, gastric, and thyroid cancers (26,27). The majority of reports indicate that IL-10 produced from immune cells may promote lung cancer growth via suppressing immune surveillance (28,29). This suppression is due to defected function of both T cells and antigen presenting cells (2).…”

Increased P-35, EBI3 Transcripts and Other Treg Markers in Peripheral Blood Mononuclear Cells of Breast Cancer Patients with Different clinical Stages

“…Thus they concluded that mass production of IL10 by macrophages facilitates tumor progression and lymph node metastasis. 24 Furthermore Merendino et al, reported a correlation between IL-10 expression levels and clinical stages of breast cancer, and suggested that NeoplasiaMetastatic Disease are associated with higher IL-10 levels compared with patients with non-metastatic diseases. 25 In this study we found a significant increase in IL-10 transcripts even at early stages of breast cancer which discovered to be stage-dependent.…”