Increased hyperalgesia by 5-nitro-2, 3-(phenylpropylamino)-benzoic acid (NPPB), a chloride channel blocker in crush injury-induced neuropathic pain in rats

Ramteke, Vinod D.; Tandan, Surendra K.; Kumar, Dinesh; Devi, R. Aruna; Shukla, Manoj K.; Prakash, Vellanki Ravi

doi:10.1016/j.pbb.2008.08.017

Cited by 13 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…), since this dose was previously reported to efficiently modulate pain threshold in animals with chronic pain. [27] NPPB or DIDS was administrated once a day without any observable side effects, and EEG was recorded simultaneously. Statistical analysis showed that the number of spontaneous status epilepticus in 0-4 hours after NPPB or DIDS injection was reduced compared to the number of seizures per 4 hours in the remaining 4-24 hours (set as baseline) respectively ( Figure 3 b, c) (b, P <0.001, paired non-parametric Wilcoxon rank test.…”

Section: Resultsmentioning

confidence: 99%

The role of voltage-gated chloride channels in the epileptogenesis of temporal lobe epilepsy

et al. 2021

View full text Add to dashboard Cite

Background: Temporal lobe epilepsy (TLE) is the most common intractable epilepsy in adults, and elucidation of the underlying pathological mechanisms is needed. Voltage-gated chloride channels (ClC) play diverse physiological roles in neurons. However, less is known regarding their functions in the epilepogenesis of TLE. Methods: ClC-mediated current and the spontaneous inhibitory synaptic currents (sIPSC) in hippocampal neurons of epileptic lesions were investigated by electrophysiological recording. The EEG data were analyzed by Z-scored wavelet and Fourier transformations. The expression of ClC-3, a member of ClC gene family, was detected by immunostaining and western blot. Findings: ClC-mediated current was increased in the hippocampal neurons of chronic TLE mice. Application of chloride channel blockers, NPPB (5-Nitro-2-[3-phenylpropylamino] benzoic acid) and DIDS (4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt) reduced ClC-mediated current and increased inhibitory synaptic transmission in TLE mice. NPPB and DIDS reduced the seizure frequency and the average absolute power of ictal high-frequency oscillations (HFOs, 80-500 Hz) in TLE mice. In addition, both drugs induced outwardly rectified currents, which might be tonic inhibitory currents in the hippocampal neurons of TLE patients. Furthermore, the expression of ClC-3 was increased in the hippocampus of TLE mice and patients and positively correlated with both the absolute power and number of ictal HFOs per seizure in the sclerotic hippocampus. Interpretation: These data suggest that ClC participate in the epilepogenetic process of TLE and the inhibition of ClC may have anti-epileptic effect.

show abstract

Section: Resultsmentioning

confidence: 99%

The role of voltage-gated chloride channels in the epileptogenesis of temporal lobe epilepsy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The paw was placed on the heat radiator with infrared intensity of lamp was set at 40. A cut of latency of 15 s was used to avoid tissue damage [22].…”

Section: Measurement Of Thermal Hyperalgesia (Paw Withdrawal Latency)mentioning

confidence: 99%

Anti-arthritic activity of Pogostemon quadrifolius

Madhiri,

Barik

2023

Preprint

View full text Add to dashboard Cite

Aim of the study: To investigate anti-arthritic activity of Aqueous methanolic extract of Pogostemon quadrifolius (AMEPQ) in Freund’s complete adjuvant (FCA)-induced arthritis in rats. Methods: The AMEPQ was prepared and subjected to acute oral toxicity in mice and tested against FCA induced arthritis in rats. Arthritis assessment was done by measuring – paw volume, joint diameter, pain threshold, thermal hyperalgesia, mechanical nociceptive threshold and body weight. Haematological, serum, biochemical and in vivo anti-oxidant parameters were measured on the last day of the study. Histopathological and radiological analyses of ankle joints were also done. MEPQ was administered at the dose of 100, 200 and 400 mg/kg body weight. Results: MEPQ dose dependently showed anti-arthritic activity which was evident with decrease in paw volume, joint diameter and increase in pain threshold, paw withdrawal latency, mechanical nociceptive threshold and body weight when compared to arthritic control group. AMEPQ (400 and 200 mg/kg) exhibits signiﬁcant (P < 0.001 and P < 0.01, respectively) anti-arthritic activity by increasing levels of RBC, Hb and by decreasing levels of WBC, platelets and also serum C-reactive protein (CRP) and Rheumatoid factor (RF). The anti-arthritic activity was also conﬁrmed with the altered biochemical parameters (AST, ALT, ALP and total protein level) and anti-oxidant parameters (SOD, MDA and GSH). MEPB (400 and 200 mg/kg) and diclofenac (10 mg/ kg) also inhibited joint destruction (histopathological and radiological analysis). Conclusion: P. quadrifolius may be a potential preventive or therapeutic candidate for the treatment of inﬂammation and arthritis.

show abstract