Increased hydrogen peroxide concentration in the exhaled breath condensate of stable COPD patients after nebulized N-acetylcysteine

Rysz, Jacek; Stolarek, Robert; Luczynski, Rafal; Sarniak, Agata; Włodarczyk, Anna; Kasielski, Marek; Nowak, Dariusz

doi:10.1016/j.pupt.2006.03.011

Cited by 24 publications

(11 citation statements)

References 35 publications

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“…However, nebulised N-acetylcysteine almost completely abolishes EBC H 2 O 2 levels 30 min after administration, although this rises almost two-fold above baseline 2.5 h later [ 95 ]. A transient increase in EBC H 2 O 2 following nebulised N-acetylcysteine has been demonstrated in patients with stable COPD [ 96 ], despite a decrease with long-term treatment [ 97 ].…”

Section: Medicationmentioning

confidence: 96%

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Since the late 1990s, a surge in interest in the analysis of exhaled breath condensate (EBC) resulted in the American Thoracic Society and European Respiratory Society (ATS/ERS) organising a Task Force in 2001 to develop guidelines on EBC collection and measurement of biomarkers. This Task Force published their guidelines in 2005 based on literature and expert opinions at that time, and multiple shortcomings and knowledge deficits were also identified. The clinical application of EBC collection and its biomarkers are currently still limited by several of these knowledge gaps, hence further guidelines for standardisation are required to ensure external validity. Using related articles produced since the publication of the ATS/ERS Task Force report, this paper attempts to provide a comprehensive update to the original guideline and review the methodological shortcomings identified. This review can hopefully serve as a yardstick for future studies involving this emerging clinical tool.

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Section: Medicationmentioning

confidence: 96%

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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“…Stimulated alveolar macrophages from asthmatics generate more reactive oxygen species than alveolar macrophages from healthy subjects [8].…”

Section: Introductionmentioning

confidence: 99%

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

Stefańska

Sokołowska

Sarniak

et al. 2010

Pulmonary Pharmacology & Therapeutics

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ARTICLE IN PRESS AbstractThe imbalance between reactive oxygen species (ROS) synthesis and antioxidants might be involved in the pathogenesis of many inflammatory diseases. NADPH oxidase, an enzyme responsible for ROS production, may represent an attractive therapeutic target to inhibit for the treatment of these diseases.Apocynin is an inhibitor of activation of NADPH oxidase complex present in the inflammatory cells.In double blind, placebo controlled, cross-over study, we investigated the effect of nebulized apocynin on ROS synthesis in 10 nonsmoking healthy volunteers. Apocynin (6 ml of 0.5 mg/ml) was administered by nebulization and its effects on H 2 O 2 , NO 2 -and NO 3 -generation were assessed after 30, 60 and 120 minutes by collecting exhaled breath condensate (EBC) samples using an EcoScreen analyzer. Additionally, respiratory parameters have been evaluated, utilizing spirometry and DLCO. We also analyzed peripheral blood differential counts and NO 2 -serum level, cough scale control and blood pressure as safety parameters. Thus, as apocynin significantly influence ROS concentration, it might have also antiinflammatory properties. As it is safe, it may have a potential to become a drug in airway inflammatory diseases treatment.

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“…EBC contains a large number of mediators [8], some of which show close correlation to airway inflammation. Elevated levels of hydrogen peroxide [9], nitrotyrosine, and nitrite [10,11] have been demonstrated in EBC in inflammatory airway diseases.…”

mentioning

confidence: 99%

Apocynin reduces reactive oxygen species concentrations in exhaled breath condensate in asthmatics

Stefańska

Sarniak

Włodarczyk

et al. 2012

Experimental Lung Research

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Asthma is an inflammatory airway disease, and oxidative stress was proven to be involved in its pathogenesis. Apocynin effectively inhibits the main source of reactive oxygen species (ROS)-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-by blocking its activation. The aim of this study was to investigate the effect of inhaled apocynin on ROS and RNS (reactive nitrogen species) concentration in 14 nonsmoking mild asthmatics. Effects of nebulized apocynin (0.5 mg/mL) were assessed in exhaled breath condensate (EBC) after 30, 60, and 120 minutes, and safety parameters have been analyzed. Apocynin significantly decreased H2O2 concentration in EBC in comparison with placebo after 60 and 120 minutes. Moreover, apocynin significantly reduced NO(-2) concentration 30 and 60 minutes after nebulization and caused a significant decrease of NO(-3) concentration in EBC 60 and 120 minutes after administration, comparing with placebo. No adverse events have been observed throughout the study. This research confirmed anti-inflammatory properties of nebulized apocynin, which might be an effective and safe drug in bronchial asthma.

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Increased hydrogen peroxide concentration in the exhaled breath condensate of stable COPD patients after nebulized N-acetylcysteine

Abstract: These data demonstrate that nebulized NAC transiently increases exhaled H(2)O(2) level, whereas it has no effect on other oxidative parameters.

Cited by 24 publications

References 35 publications

Exhaled breath condensate: a comprehensive update

Exhaled breath condensate: a comprehensive update

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

Apocynin reduces reactive oxygen species concentrations in exhaled breath condensate in asthmatics

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