Increased hsp22 RNA Levels in Drosophila Lines Genetically Selected for Increased Longevity

Kurapati, Raj; Passananti, Hardip B.; Rose, Michael R.; Tower, John

doi:10.1093/gerona/55.11.b552

Cited by 92 publications

(25 citation statements)

References 37 publications

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“…As mentioned above, that is true for Hsp70 in Drosophila (22). Flies selected for longer life display an elevated expression level of hsp22 (59), and transgenic flies overexpressing hsp22 also increase mean lifespan by 30% (60). Increased lifespan was observed by the insertion of a P element containing bidirectional UAS upstream of hsp26 genomic DNA (29).…”

Section: Figmentioning

confidence: 86%

Multiple-stress analysis for isolation ofDrosophilalongevity genes

Wang

Kazemi-Esfarjani

Benzer

2004

Proc. Natl. Acad. Sci. U.S.A.

187

173

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Long-lived organisms tend to be more resistant to various forms of environmental stress. An example is the Drosophila longevity mutant, methuselah, which has enhanced resistance to heat, oxidants, and starvation. To identify genes regulated by these three stresses, we made a cDNA library for each by subtraction of ''unstressed'' from ''stressed'' cDNA and used DNA hybridization to identify genes that are regulated by all three. This screen indeed identified 13 genes, some already known to be involved in longevity, plus candidate genes. Two of these, hsp26 and hsp27, were chosen to test for their effects on lifespan by generating transgenic lines and by using the upstream activating sequence͞GAL4 system. Overexpression of either hsp26 or hsp27 extended the mean lifespan by 30%, and the flies also displayed increased stress resistance. The results demonstrate that multiple-stress screening can be used to identify new longevity genes.aging ͉ heat-shock proteins hsp26 and hsp27 ͉ chaperones ͉ paraquat ͉ starvation G enetic or environmental manipulations to extend lifespan in various organisms have been found to correlate with increases in resistance to environmental stress (1, 2). Drosophila selected for delay in age of reproduction have increased longevity and higher resistance to many stresses, including desiccation, heat, starvation, and oxidants (3, 4), and the long-lived Drosophila methuselah and ecdysone receptor (EcR) mutants show enhanced resistance to paraquat, starvation, and heat (5, 6). The long-lived Caenorhabditis elegans mutants age-1 and daf-2 have higher resistance to oxidative stress (7), ultraviolet light (8), and heat (9, 10). Skin fibroblasts taken from long-lived p66 shc knockout mice show increased resistance to oxidative stress and UV light (11). Skin fibroblasts from mammals with varying lifespan show a positive correlation between lifespan and resistance to a variety of stressors (12). Hormesis, the beneficial effect of exposure to sublethal stress, can extend longevity in C. elegans (13) and in Drosophila (14).The correlation between stress resistance and lifespan has prompted efforts to screen for lifespan extension mutants via increased stress resistance. Several such screens have been successful, including selection for paraquat resistance in Drosophila (15) and for increased thermotolerance in C. elegans (16,17). A screen for resistance to both heat and paraquat in yeast identified mutations in adenylate cyclase and Akt͞PKB that extended stationary-phase survival 3-fold (18).The link between stress resistance and lifespan extension is further strengthened by findings that manipulation of stressresponsive genes can extend lifespan. For example, Drosophila lifespan is increased by overexpression of the antioxidant Cu-Zn superoxide dismutase (SOD) (19)(20)(21) or by overexpression of the heat-shock protein (HSP) gene hsp70 (22). In the C. elegans mutant age-1, up-regulation of Hsp16 extends lifespan (23), and heat-shock factor 1 promotes longevity as well as resistance to heat and oxidati...

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Section: Figmentioning

confidence: 86%

Multiple-stress analysis for isolation ofDrosophilalongevity genes

Wang

Kazemi-Esfarjani

Benzer

2004

Proc. Natl. Acad. Sci. U.S.A.

187

173

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“…Besides possible interactions with elements of the cytoskeleton (85)(86)(87), different members of the chaperone family are found to be present at sites of inflammation (88,89) and cellular defects like ischemic acute renal failure (90) or excitotoxic lesions (91). As a general protective effect, it was reported that high levels of sHSPs extend the life span of some organisms (92)(93)(94). In Synechocystis a sHSP (Hsp17) was found to be involved in membrane stabilization (95,96), in Chlamydomonas Hsp-22 in photosystem protection (97) and AtHsp17.8-CI in Arabidopsis facilitated the protein reallocation from the cytosol to the outer chloroplast membrane (98).…”

Section: Localization and Physiological Relevancementioning

confidence: 99%

Small heat shock proteins: recent developments

Eisenhardt

2013

BioMolecular Concepts

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Small heat shock proteins (sHSPs) are abundantly present in many different organisms at elevated temperatures. Members of the subgroup of alpha crystallin domain (ACD)-type sHSPs belong to the large family of protein chaperones. They bind non-native proteins in an ATP-independent manner, thereby holding the incorporated clients soluble for subsequent refolding by other molecular chaperoning systems. sHSPs do not actively refold incorporated peptides therefore they are sometimes referred to as holdases. Varying numbers of sHSPs have been documented in the different domains of life and dependent on the analyzed organism. Generally, diverse sHSPs possess more sequence similarities in the conserved ACD, whereas the N- and C-terminal extensions are less conserved. Despite their designation as sHSPs, they are not solely present during heat stress. sHSPs presumably help to protect cells under various stresses, but they were also found during development, e.g., in embryonic development of higher plants which is associated with ongoing seed desiccation. The functional and physiological relevance of several different sHSPs in one organism remains still unclear, especially in plants where several highly similar sHSPs are present in the same compartment. The wide range of biotic and abiotic stresses that induce the expression of multiple sHSP genes makes it challenging to define the physiological relevance of each of these versatile proteins.

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“…Researches have been carried out to demonstrate that a ubiquitous or a targeted up-regulated expression of HSP22 or increased HSP22 RNA levels [40] correlated with the increased mean life span of Drosophila, better tolerance to high temperature [41], more beneficial effects on early-aging events since the premortality, longer locomotor activity and significantly increase in flies' resistance to oxidative injuries induced by paraquat and thermal stress [42], while resistance to coumarin poisoning was not affected [43]. Meanwhile, flies that are not expressing this mitochondrial HSP22 display a decrease (≥30%) both in lifespan and locomotor activity and sensitization to mild stress compared with controls [44].…”

Section: Lifespan Extension and Antioxidationmentioning

confidence: 99%

HSP22 and its Role in Human Neurological Disease

Guan

Tu²,

Li³

et al. 2011

CNR

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HSP22 (heat shock protein 22), belonging to the superfamily of small heat shock proteins, which has a molecular mass of 21.6 KD and is able to exist in the form of monomer, has multiple functions including molecular chaperones, apoptosis and anti-apoptosis, lifespan extension, antioxidation and so on. In recent years, studies show that HSP22 plays a crucial role in many neurological diseases, such as hereditary nerve endings disease, Alzheimer disease and Charco-Marie-Tooth. This review explores the progress in HSP22 and its involvement in human neurological disease.

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Increased hsp22 RNA Levels in Drosophila Lines Genetically Selected for Increased Longevity

Cited by 92 publications

References 37 publications

Multiple-stress analysis for isolation ofDrosophilalongevity genes

Multiple-stress analysis for isolation ofDrosophilalongevity genes

Small heat shock proteins: recent developments

HSP22 and its Role in Human Neurological Disease

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