Increased Heat Shock Protein Expression after Stress in Japanese Quail

Abstract

Heat shock proteins (HSPs) have been shown to provide information on the biological impact of environmental stress to organisms, yet none have investigated the HSP response to stress in birds. Japanese quail were exposed to seven different stressors (mild restraint, loud noise, inescapable irritation, cold temperature, isolation in darkness, and two stressful social situations) and expression of HSP30, 60, 70, and 90 in heart, liver, lung, kidney and gonads was examined. Tonic Immobility (TI) tests were also conducted to assess whether the stressors increased fear response. Increased expression of HSP70 was found in the myocardial tissue of birds exposed to loud noise, inescapable irritation, cold temperature, and isolation in darkness. Increased expression of other HSPs was not apparent in the heart or any of the other all tissues examined. Longer TI was observed only in birds exposed to the noise stress. Evidence is presented that a fairly wide range of stressors caused increased expression of HSP70 in the Japanese quail myocardial tissue and that HSPs may provide useful biomarkers for the study of environmental stress in birds.

Physiological and agonistic behavioural response of Procambarus clarkii to an acoustic stimulus

“…Little is known about the effects of noise on Hsp expression. Wu et al (Wu et al, 2001) showed that Hsp70 expression increased after exposure to a stressful noise in humans, and Hoekstra et al (Hoekstra et al, 1998) reported that the expression of Hsp70 (but not Hsp30, Hsp60 or Hsp90) is increased in birds after exposure to a loud noise. We have also recently detected increased Hsp70 expression in the fish Chromis chromis after exposure to sounds similar to those resulting from human activities (M.V., M.C., D. Arizza, G. Calandra, G.B., D.P., V. Ferrantelli, C. Bracciali and G. Sarà, unpublished).…”

Behavioural and biochemical stress responses of Palinurus elephas after exposure to boat noise pollution in tank

“…In crustaceans, the expression of Hsp70 serves as a good bioindicator of stressful conditions, including pesticide exposure and heat stress (Snyder and Mulder, 2001;Liberge and Barthélémy, 2007;). Only a few studies have examined the effects of noise on Hsp expression (Hoekstra et al, 1998;Wu et al, 2001;Celi et al, 2013). Recently, increased Hsp70 expression levels were detected in Chromis chromis after exposure to sounds similar to those resulting from human activities (Celi et al, unpublished).…”

“…Little is known about the effects of noise on Hsp expression. Wu et al (2001) showed that Hsp70 expression increased after exposure to a stressful noise in humans, and Hoekstra et al (1998) reported that the expression of Hsp70 (but not Hsp30, Hsp60 or Hsp90) is increased in birds after exposure to a loud noise.…”

Initial transcriptome and proteome analyses of low culture temperature‐induced expression in CHO cells producing erythropoietin

“…It interacts with newly synthesized glycoproteins and putative chaperones such as calnexin and calreticulin. Heat shock cognate 71 kDa protein is a cognate form of HSP70, and one of the HSP70 family proteins, which helps to fold many proteins (Hoekstra et al, 1998;Laios et al, 1997). Hsp70assisted folding involves repeated cycles of substrate binding and release.…”