Increased H2 production in the cyanobacterium Synechocystis sp. strain PCC 6803 by redirecting the electron supply via genetic engineering of the nitrate assimilation pathway

Baebprasert, Wipawee; Jantaro, Saowarath; Khetkorn, Wanthanee; Lindblad, Peter; Incharoensakdi, Aran

doi:10.1016/j.ymben.2011.07.004

Cited by 96 publications

(52 citation statements)

References 25 publications

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“…nutrients and light; Kim et al, 2011;Lea-Smith et al, 2014;Burnap, 2015;Touloupakis et al, 2015;van Alphen and Hellingwerf, 2015). Understanding of the factors controlling the limitation of Synechocystis growth would facilitate the use of this strain as a cell factory (Yu et al, 2013) for the production of biomass (Joseph et al, 2014), pigments (Sekar and Chandramohan, 2008), secondary metabolite natural products (Frommeyer et al, 2016), biofuel (Dexter and Fu, 2009;Baebprasert et al, 2011;Liu et al, 2011), and other high-value compounds.…”

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confidence: 99%

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

et al. 2017

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ORCID IDs: 0000-0001-7618-4748 (M.I.); 0000-0001-8931-7722 (T.O.); 0000-0002-6113-9570 (R.S.).Many studies have investigated the various genetic and environmental factors regulating cyanobacterial growth. Here, we investigated the growth and metabolism of Synechocystis sp. PCC 6803 under different nitrogen sources, light intensities, and CO 2 concentrations. Cells grown on urea showed the highest growth rates. However, for all conditions tested, the daily growth rates in batch cultures decreased steadily over time, and stationary phase was obtained with similar cell densities. Unexpectedly, metabolic and physiological analyses showed that growth rates during log phase were not controlled primarily by the availability of photoassimilates. Further physiological investigations indicated that nutrient limitation, quorum sensing, light quality, and light intensity (self-shading) were not the main factors responsible for the decrease in the growth rate and the onset of the stationary phase. Moreover, cell division rates in fed-batch cultures were positively correlated with the dilution rates. Hence, not only light, CO 2 , and nutrients can affect growth but also a cell-cell interaction. Accordingly, we propose that cell-cell interaction may be a factor responsible for the gradual decrease of growth rates in batch cultures during log phase, culminating with the onset of stationary phase.

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confidence: 99%

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

et al. 2017

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“…Several bottlenecks have been identified, some of which have been addressed: (i) H 2 uptake by the cells (uptake hydrogenase)-genetically engineered strains with a non-functional/deleted uptake hydrogenase have been produced (see Tamagnini et al 2007), (ii) low energy efficiency and turnover of the nitrogenase and/or the hydrogenase, (iii) limiting amounts of active H 2 -evolving enzymes, (iv) high oxygen sensitivity of the nitrogenase and/or the hydrogenase, (v) electron flow inhibition by accumulation of ATP in a hydrogenase-driven system, (vi) low quantum efficiency due to too large antennas in both Photosystem II (PSII) and PSI, and (vii) electronconsuming pathways competing with an efficient electron transfer to the H 2 enzymes (Tamagnini et al 2007;Angermayr et al 2009). Addressing the last point, a significantly increased hydrogen production was recently achieved when redirecting the electron supply toward the hydrogenase via genetic engineering of the electron-competing nitrate assimilation pathway in the unicellular cyanobacterium Synechocystis 6803 (Baebprasert et al 2011). Recently, the bacterial [FeFe] hydrogenase HydA from Clostridium acetobutylicum has been expressed in Synechococcus elongatus PCC 7942 together with the necessary maturation enzymes, and, under anoxic conditions, the resulting light-dependent hydrogen evolution could be shown to be increased [500 times, compared to H 2 evolution from the endogenous [NiFe] hydrogenase (Ducat et al 2011a).…”

Section: Native and Engineered Cyanobacteria For Photobiological H 2 mentioning

confidence: 99%

“…Cyanobacteria have been genetically engineered and demonstrated to produce H 2 (Tamagnini et al 2007;Baebprasert et al 2011;Ducat et al 2011a), ethanol (Deng and Coleman 1999;Dexter and Fu 2009), 1-butanol (Lan and Liao 2011), isobutyraldehyde and isobutanol (Atsumi et al 2009), isoprene (Lindberg et al 2010), ethylene (Takahama et al 2003), sugars, and lactic acid (Niederholtmeyer et al 2010)-for recent reviews, see Angermayr et al (2009) and Ducat et al (2011b).…”

Section: Examples Of Genetically Engineered Cyanobacteria Producing Amentioning

confidence: 99%

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production

Lindblad

Lindberg

Oliveira³

et al. 2012

AMBIO

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“…Previous studies had pointed that inhibiting (Gutthann et al, 2007) or disrupting (Baebprasert et al, 2011) the nitrate assimilation pathway and feeding ammonia to Synechocystis, instead of nitrate or nitrite, increased hydrogen production was achieved ( Figure 4.5 (A) and (B)).…”

Section: Assessment Of Nitrogen and Sulphur Substrates To Enhance H 2mentioning

confidence: 90%

“…Previous studies from Gutthann et al (2007) and Baebprasert et al (2011) pointed to the use of different sources of nitrogen. In the case of sulphur, no previous study was found in literature in which different species were tested for hydrogen production.…”

Section: Assessment Of Nitrogen and Sulphur Substrates To Enhance H 2mentioning

confidence: 99%

Modelling and multiobjective optimization for simulation of cyanobacterial metabolism

Paula¹

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AcknowledgementsEn primer lugar, quisiera dar las gracias a aquellos que me brindaron la oportunidad de emprender esta aventura y me han acompañado durante el proceso para que pudiese llevarla a buen término. Gracias a Pedro Fernández de Córdoba y a Javier Urchueguía por abrirme las puertas y permitirme adentrarme en este proyecto. Gracias Pedro por allanar siempre el camino y tener a mano unas palabras de aliento. Gracias Javier por plantear retos y preguntas y estar siempre pendiente de las respuestas. Gràcies a Arnau Montagud, per formar-me i tutelar-me en la meua introducció a la investigació. Gràcies per fomentar sempre el meu esperit crític, per extraure de mi la solució més encertada, i per donar-me el teu consell tant en temes científics i acadèmics, com en alguns altres més personals. Gracias a Gilberto Reynoso, por compartir conmigo su sapiencia y experiencia. Aunque llegaste un poco más tarde, has resultado ser un elemento fundamental para este trabajo, y sin duda también para mi desarrollo científico y personal, gracias por las largas charlas y los buenos consejos. Quiero dar las gracias también a Alberto Conejero y a Daniel Gamermann, que en distintos momentos han aportado su dedicación y su experiencia a este trabajo.No me puedo olvidar de mi compañero de batallas, David Fuente. Gracias por compartir la carga en los buenos y no tan buenos momentos, y por estar siempre a punto para compartir una duda, un café o lo que se tercie. Tampoco puedo dejar de lado a aquellos que me han acompañado en mi paso por la 210, Joan Vázquez, Andrea Vázquez, Ramón Jaime, Borja Badenes, (y tantos otros, perdonad que no os mencione a todos) con los que he convivido a diario en distintos periodos y que siempre me han ofrecido lo mejor de su compañía, a veces discusiones trascendentales, otras veces divertidas charlas ligeras.I would like to thank also Röbbe Wünschiers, for welcoming me in his research group (even in your home). Röbbe, I have to thank you for giving me a broader (and so great!) perspective of science world and science geniuses. I thank also Gabriel Kind, who accompanied me in most of my international experiences during this process. Thank you Gabriel for accepting my way of doing things, and for offering me your friendship, it was always comfortable to work and live with you. And I would like to thank Andrew Landels too. "Professor" Landels (I hope my English is good enough) I thank you for sharing your knowledge and personality with me, you were soon a friend, more than a colleague.vi Per suposat, també he d'agrair els meus amics, que han aguantat (i aguantaran) les meues "frikades", i sempre han estat a punt per a una cervesa (i el que vinga) quan els he necessitat. Ferran, potser no ens veiem tant com voldriem, però sé que sempre estàs ahí, i que tens paciència infinita amb mi; gràcies. David i Neus, vosaltres també teniu el vostre lloc ací, gràcies per ser més que família, per compartir moments divertits i entranyables, cantant, esquiant o, simplement, estant. David (sí, señor Terrel), a ti también t...

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Increased H2 production in the cyanobacterium Synechocystis sp. strain PCC 6803 by redirecting the electron supply via genetic engineering of the nitrate assimilation pathway

Cited by 96 publications

References 25 publications

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production

Modelling and multiobjective optimization for simulation of cyanobacterial metabolism

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