Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones

Swaaij, A.C. van; Nijdam, H. F.; Jacobsen, E.; Feenstra, W.J.

doi:10.1007/bf00041480

Cited by 24 publications

(7 citation statements)

References 29 publications

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“…Proline levels can be manipulated in some species by in vitro selection in the presence of hydroxyproline (Van Swaaij et al 1986, 1987Dix 1993). Hydroxyproline is a toxic analogue of the amino acid proline and selection in the presence of hydroxyproline can lead to proline over-accumulation in cell lines and eventually to plants with heritable stress resistance (Riccardi et al 1983;Dorffling et al 1997).…”

Section: Introductionmentioning

confidence: 98%

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

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Plant Cell Tiss Organ Cult

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An efficient in vitro screening method has been developed for cauliflower to create NEU and NMU induced mutant lines selected on hydroxyproline containing medium. Mutant lines and control plants were sub-cultured many times on maintenance medium and stored at 5°C for 2 years and then tested for salt and hydroxyproline resistance as in vitro and in vivo plants. In vitro shoot tips were also sub-cultured to media containing hydroxyproline and NaCl for 28 days and then assessed for their leaf proline content. Non-acclimated and acclimated in vivo plants were also assessed for resistance to freezing. Populations of control and selected lines were created by mass pollination and subsequently tested for their NaCl and frost resistance. Control plants had little or no NaCl or hydroxyproline resistance whilst selected plants showed varying degrees of resistance. In vitro and in vivo responses of selected lines were correlated. Leaf proline content was increased markedly in the mutant lines and the greatest proline contents occurred following NaCl stress with the most respondent line having 100-fold levels compared to the controls. Both non-acclimated and acclimated selected lines showed improved frost resistance over controls. Improvements in frost resistance were heritable but improvements in NaCl resistance were not. The results clearly demonstrated that NaCl, frost and hydroxyproline resistance were stable traits over repeated in vitro sub-cultures and prolonged low temperature storage. A complete range of mutants with single, double or triple resistance traits were produced. The level of resistance however was not necessarily correlated with the level of proline and some lines showed resistance without elevated proline. It is concluded that elevated proline is not essential for improved resistance to abiotic stress in cauliflower, but where it does occur it does improve resistance.

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Section: Introductionmentioning

confidence: 98%

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

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“…tuberosum tuber cannot survive a temperature of -3°C and lower. The foliage dies at temperatures of -4°C (van Swaaij et al, 1987;Vayda, 1994). Dale (1992) reports that potato tubers are destroyed by a frost period of 25 hours at -2°C or a frost period of five hours at -10°C.…”

Section: A Cultivationmentioning

confidence: 99%

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

2006

Safety Assessment of Transgenic Organisms, Volume 1

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The OECD is a unique forum where the governments of 30 democracies work together to address the economic, social and environmental challenges of globalisation. The OECD is also at the forefront of efforts to understand and to help governments respond to new developments and concerns, such as corporate governance, the information economy and the challenges of an ageing population. The Organisation provides a setting where governments can compare policy experiences, seek answers to common problems, identify good practice and work to co-ordinate domestic and international policies.The OECD member countries are: Australia, Austria, Belgium, Canada, the Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Japan, Korea, Luxembourg, Mexico, the Netherlands, New Zealand, Norway, Poland, Portugal, the Slovak Republic, Spain, Sweden, Switzerland, Turkey, the United Kingdom and the United States. The Commission of the European Communities takes part in the work of the OECD.OECD Publishing disseminates widely the results of the Organisation's statistics gathering and research on economic, social and environmental issues, as well as the conventions, guidelines and standards agreed by its members. © OECD 2006 No reproduction, copy, transmission or translation of this publication may be made without written permission. Applications should be sent to OECD Publishing: rights@oecd.org or by fax (33 1) 45 24 13 91. Permission to photocopy a portion of this work should be addressed to the Centre français d'exploitation du droit de copie, 20, rue des Grands-Augustins, 75006 Paris, France (contact@cfcopies.com). This book is published on the responsibility of the Working Group on Harmonisation of Regulatory Oversight in Biotechnology, which is a subsidiary group of the Chemicals Committee and Working Party on Chemicals, Pesticide and Biotechnology of the OECD. Foreword 3 FOREWORDGenetically engineered crops (also known as transgenic crops) such as maize, soybean, rapeseed and cotton have been approved for commercial use in an increasing number of countries. During the period from 1996 to 2005, for example, there was more than fifty-fold increase in the area grown with transgenic crops worldwide, reaching 90 million hectares in 2005.1 Such approvals usually follow a science-based risk/safety assessment.The environmental safety/risks of transgenic organisms are normally based on the information on the characteristics of the host organism, the introduced traits, the environment into which the organism is introduced, the interaction between these, and the intended application. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology decided at its first session, in June 1995, to focus its work on identifying parts of this information, which could be commonly used in countries for environmental safety/risk assessment to encourage information sharing and prevent duplication of effort among countries. Biosafety Consensus Documents are one of the major outputs of its work.B...

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“…Amino acid analogue-resistant mutants which overproduce the specific amino acid have been found in bacteria (Czonka, 1981 ;Sugiura & Kisumi, 1985) as well as plants (Widholm, 1976 ;Kueh & Bright, 1982 ;Van Swaaij et al ., 1986 ;Mori et al ., 1989) . It has been reported that free proline-accumulating cell lines resistant to a proline analogue show increased resistance to stresses such as salt (Riccardi et al ., 1983) and freezing (Van Swaaij et al ., 1986, 1987 . Van Swaaij et al .…”

Section: Introductionmentioning

confidence: 99%

Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower (brassica oleracea var. botrytis)

1995

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A procedure is described by which hydroxyproline-resistant lines could be selected from regenerating curd tissue of cauliflower. Mutagenesis was by N-nitroso-N-ethylurea, supplied as a drop of 0 .3 mM solution on each 3 mm diameter curd piece. The mutagen generated numerous morphological and pigment mutations without significantly affecting shoot regeneration from explants . Thirty one resistant shoots were recovered from more than six thousand explants mutagenised on regeneration medium supplemented with 3 mM hydroxyproline, while none was obtained from a similar number of non-mutagenised controls . Out of twenty-three resistant shoots which survived subculture, only one showed consistently elevated levels of endogenous proline . During early shoot culture passages, proline levels were 3 .6-4.7 times higher than controls, but this was reduced to 1 .6 times after 10-12 culture passages in the absence of hydroxyproline . Possible reasons for this decline are discussed . Leaf strip assays suggest resistant shoots may be chimeras and current efforts are directed towards regenerating solid mutants from resistant sectors . These will then be evaluated for any alteration in frost tolerance .Abbreviations : IBA -Indole-3-butyric-acid, NEU -N-nitroso-N-ethylurea

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Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones

Cited by 24 publications

References 29 publications

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Section 7 - Bread Wheat (TRITICUM AESTIVUM)

Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower (brassica oleracea var. botrytis)

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