Increased expression of tumor necrosis factor-α is associated with advanced colorectal cancer stages

Abstract

METHODS:Archival tissue specimens were collected from 30 patients with CRC who had undergone surgical intervention at King Khalid University Hospital. Patient demographic information, including age and gender, tumor sites, and histological type of CRC, was recorded. To measure TNF-a mRNA expression in CRC, total RNA was extracted from tumor formalin-fixed, paraffinembedded, and adjacent normal tissues. Reverse transcription and reverse transcription polymerase chain reaction were performed. Colorectal tissue microarrays were constructed to investigate the protein expression of TNF-a by immunohistochemistry. RESULTS:The relative expression of TNF-a mRNA in colorectal cancer was significantly higher than that seen in adjacent normal colorectal tissue. High TNF -a gene expression was associated with Stage Ⅲ and Ⅳ neoplasms when compared with earlier tumor stages (P = 0.004). Eighty-three percent of patients (25/30) showed strong TNF-a positive staining, while only 10% (n = 3/30) of patients showed weak staining, and 7% (n = 2/30) were negative. We showed the presence of elevated TNF-a gene expression in cancer cells, which strongly correlated with advanced stages of tumor. CONCLUSION:High levels of TNF-a expression could be an independent diagnostic indicator of colorectal cancer, and targeting TNF-a might be a promising prognostic tool by assessment of the clinical stages of CRC.

Protein Expression and Genetic Variation of IL32 and Association with Colorectal Cancer in Swedish Patients

“…We investigated the concentration of these cytokines in lysates of CRC and paired normal tissue and found a statistically significantly higher concentration in cancer tissue. These findings are consistent with data from previous studies (33)(34)(35). Interestingly, we also noted a statistically significantly positively correlation between the level of IL32 and these cytokines.…”

Enhancement of colon carcinogenesis by the combination of indole-3 carbinol and synbiotics in hemin-fed rats

“…TNF has been associated with a wide range of biological activities, including inflammation and cell differentiation (Sedger and McDermott, 2014). Studies have demonstrated that over expression of TNF in CRC can be associated with tumor progression, invasion, and metastasis (Al Obeed et al, 2014). Thus, aberrant Ecadherin and TNF gene expression is consistent with the increased tumor invasiveness observed in our study in the group fed hemin + I3C.…”

The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats

“…Administration of DMH induced colon cancer in rats exhibited significant increase in TNF-alpha colon tissues (Table2). The relative expression of TNF-α mRNA in colorectal cancer was significantly higher than that present in adjacent normal colorectal tissue (Al Obeed et al, 2014).Due to metabolism of DMH, the production of excess ROS ends in the activation of TNF-α by p65-NF-ĸB pathway. Enhanced level of TNF-α could damage function and transcription of the affected proteins and progression of epithelial cell transformation to invasive cancer in colonic mucosa through initiating the inflammation and progression of cancer (Anna et al, 2015 andGarza-Trevino et al, 2015).…”