Increased expression of PTX3 in non-hematopoietic periosteal cells during fracture healing

Kelava, Tomislav; Ivčević, Sanja; Katavić, Vedran; Kovačić, Nataša; Cvija, Hrvoje; Petrović, Katerina Zrinski; Kuzmac, Sania; Kalajzić, Ivo; Bottazzi, Barbara; Grčević, Danka

doi:10.1530/boneabs.3.pp68

Cited by 3 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Noteworthy, the expression of PTX3 appears significantly reduced in osteoblasts that showed a poor propensity to form hydroxyapatite crystals, especially in osteoporotic patients. The correlation between osteoblast PTX3 deficiency and low bone density that we are observing in human subject well matches with bone structure deficiency and impaired fracture healing described in the animal model of PTX3 null mice 6 . Specifically, Authors reported that bone regeneration requires PTX3 for both the induction of the fracture healing process and recruitment of differentiated osteoblasts 6 .…”

supporting

confidence: 86%

“…The correlation between osteoblast PTX3 deficiency and low bone density that we are observing in human subject well matches with bone structure deficiency and impaired fracture healing described in the animal model of PTX3 null mice 6 . Specifically, Authors reported that bone regeneration requires PTX3 for both the induction of the fracture healing process and recruitment of differentiated osteoblasts 6 . Data now available about the relationship between PTX3 and bone, allow to hypothesize the mechanisms through which PTX3 could be involved in both bone metabolism and osteoporosis development (Fig.…”

supporting

confidence: 86%

“…Specifically, it has been described that PTX3 is able to induce the expression of Receptor Activator of Nuclear factor Kappa-B Ligand (RANKL) by osteoblasts promoting osteoclasts activation in an in vitro culture system 5 . On the other hand, preliminary data by Kelava et al investigated the relationship between PTX3 expression and bone quality in mice deficient for the PTX3 gene demonstrating that PTX3 null mice had lower bone mass than their WT littermates, implying a positive involvement of PTX3 in bone formation rather than in the resorption 6 . PTX3 is considered as a multifunctional glycoprotein involved in cell proliferation and migration, and deposition and remodelling of the extracellular matrix by a variety of cells such as vascular endothelial cells, smooth muscle cells, fibroblasts, adipocytes, chondrocytes, mesangial, epithelial and mesenchymal stromal cells 7,8 .…”

mentioning

confidence: 99%

See 2 more Smart Citations

PTX3: a new mediator of bone metabolism and osteoporosis

Bano-Aledo

Feola

Celi

et al. 2019

Muscle Ligaments and Tendons J

View full text Add to dashboard Cite

supporting

confidence: 86%

supporting

confidence: 86%

mentioning

confidence: 99%

See 1 more Smart Citation

PTX3: a new mediator of bone metabolism and osteoporosis

Bano-Aledo

Feola

Celi

et al. 2019

Muscle Ligaments and Tendons J

View full text Add to dashboard Cite

“…Given its recently emerging role, attention has been focused on PTX3 involvement in bone homeostasis. Particularly, great interest has been shown for its involvement in the pathogenesis of chronic bone diseases such as osteoporosis and in the fracture healing process, both in mice and humans (13,14,17,18,20,33). It has been shown that PTX3 is able to induce Receptor Activator of Nuclear factor Kappa-B Ligand (RANKL) expression by osteoblasts that promote the activation of osteoclasts in an in vitro culture system (17).…”

Section: Ptx3 Osteoporosis and Fracture Healingmentioning

confidence: 99%

“…These results suggest that PTX3 can act as an inflammatory mediator that contributes to the deterioration of the osteolytic conditions of the inflamed bone. On the other hand, it has been proposed that PTX3 is involved in bone formation rather than resorption, as demonstrated by Kelava et al in 2014, who studying the relationship between PTX3 expression and bone quality in mice deficient for the PTX3 gene, demonstrated that PTX3 null mice had lower bone mass than wild-type mice (18). To establish the effective involvement of PTX3 in bone pathophysiology, its expression and function in human osteoblasts of osteoporotic, osteoarthritic patients and young subjects not affected by bone pathologies were studied (14).…”

Section: Ptx3 Osteoporosis and Fracture Healingmentioning

confidence: 99%

The Role of PTX3 in Mineralization Processes and Aging-Related Bone Diseases

et al. 2021

View full text Add to dashboard Cite

The Long Pentraxin 3 (PTX3) is a multifunctional glycoprotein released by peripheral blood leukocytes and myeloid dendritic cells in response to primary pro-inflammatory stimuli, that acts as a non-redundant component of the humoral arm of innate immunity. In addition to the primary role in the acute inflammatory response, PTX3 seems to be involved in other physiological and pathological processes. Indeed, PTX3 seems to play a pivotal role in the deposition and remodeling of bone matrix during the mineralization process, promoting osteoblasts differentiation and activity. Recently, PTX3 was seen to be involved in the ectopic calcifications’ formation in breast cancer disease. In this regard, it has been observed that breast cancer tumors characterized by high expression of PTX3 and high amount of Breast Osteoblast Like Cells (BOLCs) showed several Hydroxyapatite (HA) microcalcifications, suggesting a likely role for PTX3 in differentiation and osteoblastic activity in both bone and extra-bone sites. Furthermore, given its involvement in bone metabolism, several studies agree with the definition of PTX3 as a molecule significantly involved in the pathogenesis of age-related bone diseases, such as osteoporosis, both in mice and humans. Recent results suggest that genetic and epigenetic mechanisms acting on PTX3 gene are also involved in the progression of these diseases. Based on these evidences, the aim of our systemic review was to offer an overview of the variety of biological processes in which PTX3 is involved, focusing on bone mineralization, both in a physiological and pathological context.

show abstract

Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis

et al. 2017

View full text Add to dashboard Cite

Pentraxin 3 (PTX3) is a multifunctional glycoprotein regulating inflammatory response, cell proliferation and migration and deposition and remodelling of the extracellular matrix by a variety of cells. In this study, we investigated the possible role of PTX3 in bone homeostasis. To this end, we compared the expression and function of PTX3 in human osteoblasts of osteoporotic, osteoarthritic patients and young subjects not affected by bone diseases. Immunohistochemical analysis performed on bone head biopsies showed a close association between bone health and the number of osteoblasts expressing PTX3. Noteworthy, the proportion of PTX3-positive osteoblasts resulted to be significantly lower in osteoporotic patients compared with both young patients and osteoarthritic patients of the same age. Ex vivo culture of osteoblasts isolated from the three groups of patients confirmed in vivo observation. Specifically, we observed rare runt-related transcription factor 2 (RUNX2) immunopositive osteoblasts expressing PTX3 in cell cultures derived from osteoporotic patients and western blotting analysis showed 80% reduction of PTX3 in the corresponding culture extracts compared with young and osteoarthritic patients. The treatment of human osteoblast primary cultures derived from young patients with anti-PTX3 antibody dramatically affected osteoblast behaviour. Indeed, they lost the morphological and molecular features typical of mature osteoblasts, acquiring fibroblast-like shape and highly decreasing nuclear factor kappa-B ligand (RANKL) and RUNX2 expression. Also, the inhibition of PTX3 negatively affected osteoblast proliferation and their ability to form cell clusters and microhydroxyapatite crystals. Altogether, these results suggest a central role of PTX3 in bone homeostasis showing its involvement in osteoblast proliferation, differentiation and function.

show abstract

Increased expression of PTX3 in non-hematopoietic periosteal cells during fracture healing

Cited by 3 publications

References 0 publications

PTX3: a new mediator of bone metabolism and osteoporosis

PTX3: a new mediator of bone metabolism and osteoporosis

The Role of PTX3 in Mineralization Processes and Aging-Related Bone Diseases

Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis

Contact Info

Product

Resources

About