Increased expression of p63 and survivin in cholesteatomas

Park, H.R.; Min, Soo Kee; Min, Kyoung‐jin; Jun, Sun-Young; Seo, Juyeon; Kim, H.J.

doi:10.1080/00016480802251591

Cited by 16 publications

(17 citation statements)

References 18 publications

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“…Park et al (18) determined that in cholesteatoma, survival is significantly increased; hence, apoptosis is suppressed in such cases. Olszewska et al (19) Turk Figure 4.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Isik

Karlıdağ

Şimşek

et al. 2015

Turk Arch Otorhinolaryngol

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Objective: The aim of this study was to evaluate bcl-2, bax, and c-erbB-2 expressions in primary and secondary acquired cholesteatoma and to indicate the role of apoptosis and accompanying increased cellular proliferation in the pathogenesis of cholesteatoma.Methods: Samples obtained from the skin of the external ear canal (EEC) of patients operated for chronic otitis media (COM) without cholesteatoma constituted Group 1; samples from the EEC skin of patients in Group 3 operated for COM with cholesteatoma and from the EEC skin of patients in Group 4 constituted Group 2; samples obtained from the cholesteatoma matrix of patients operated for COM with primary acquired cholesteatoma constituted Group 3; and samples obtained from the cholesteatoma matrix of patients operated for COM with secondary acquired cholesteatoma constituted Group 4. The assessment of the positive cell ratio was based on the presence of the following findings and was semiquantitatively classified into four groups: 0, no staining; + cell staining (weak positive staining: 1%-33%); ++ cell staining (moderately positive staining: 34%-66%); and +++ cell staining (strong positive staining: 67%-100%). Results:Comparison of the staining scores of bcl-2, bax, and c-erbB-2 revealed a statistically insignificant difference in the staining of samples obtained from the EEC skin (p>0.05). Decreased bcl-2 expression and increased bax and c-erbB-2 expressions were determined in primary and secondary acquired cholesteatoma epithelium compared with the EEC skin of patients operated for COM with or without cholesteatoma, and the differences were found to be statistically significant (p<0.05). Conclusion:In acquired cholesteatoma epithelium, the finding of decreased bcl-2 expression as well as increased bax and c-erbB-2 expressions compared with the EEC skin is an indicator of the increase in both cellular proliferation and apoptosis.Keywords: Cholesteatoma, apoptosis, cell proliferation, bcl-2, bax, c-erbB-2

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“…Park et al (18) determined that in cholesteatoma, survival is significantly increased; hence, apoptosis is suppressed in such cases. Olszewska et al (19) Turk Figure 4.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Isik

Karlıdağ

Şimşek

et al. 2015

Turk Arch Otorhinolaryngol

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“…However, research using cell markers has confirmed that migration plays a role during normal or precholesteatoma conditions. 48 Other investigations have shown that metaplasia of mucosal epithelium into lightly keratinising squamous epithelium like masses can only be feasible in the absence of congenital remnants, skull trauma or previous middle-ear surgery. 56 Cholesteatoma pathogenesis: stepwise explanations Although different views on the migration, hyperplasia and metaplasia theories of acquired cholesteatoma formation have been expounded in the literature, it is conceivable that a combination of these theories may contribute to the pathogenesis of acquired cholesteatoma.…”

Section: Epidermal Hyperplasiamentioning

confidence: 99%

Acquired cholesteatoma pathogenesis: stepwise explanations

Louw

2010

J. Laryngol. Otol.

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The triggers for cholesteatoma onset are diverse, and may involve tympanic membrane trauma (i.e. perforation, displacement, retraction or invagination), tympanic membrane disease, and/or tympanic cavity mucosa disease. Research has revealed that cell migration is replaced under inflammatory conditions by hyperplasia, which triggers the onset of cholesteatoma. Lately, the hyperplasia theory gained prominence and circumscription of the papillary cone formation concept provided insight into cholesteatoma progression (growth and expansion). Diseased mucosa can contribute to the development of retraction pockets and cholesteatoma. The type of cholesteatoma trigger and the role of chronic inflammation during disease progression and recurrence are important in guiding clinical intervention.

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“…A variety of studies have attempted to differentiate the underlying pathogenesis and origin between congenital and acquired cholesteatomas [19,20] . It is known that, unlike in acquired cholesteatomas, bacterial infection is not involved in the development of the congenital type.…”

Section: Discussionmentioning

confidence: 99%

Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma

Haruyama

Furukawa²,

Kusunoki³

et al. 2010

ORL

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Purpose: The present study was designed to elucidate the immunoreactivity and protein level of IL-17 in human cholesteatomas. Procedures: The expression and localization of IL-17 and receptor activator of nuclear factor ĸB ligand (RANKL) were examined by immunohistochemistry in tissue specimens collected from 24 patients with cholesteatomas. The cellular sources of IL-17 were assessed by double staining with CD4. The level of IL-17 protein was determined using an enzyme-linked immunosorbent assay. The degree of bone destruction was compared with the IL-17 immunoreactivity. Results: IL-17 immunoreactivity was detected in the inflammatory cells in the granulation tissues and there were increased levels of IL-17 protein. The localization of IL-17 expression coincided with CD4-positive lymphocytes. IL-17 was correlated with the cells positive for RANKL. The degree of bone destruction was dependent on the number of infiltrated IL-17-positive cells. IL-17-driven pathology was observed in the congenital type as compared with the acquired type. Conclusions: The present study suggests that IL-17 is related to the pathogenesis of the disease.

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Increased expression of p63 and survivin in cholesteatomas

Cited by 16 publications

References 18 publications

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Acquired cholesteatoma pathogenesis: stepwise explanations

Expression of IL-17 and Its Role in Bone Destruction in Human Middle Ear Cholesteatoma

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