Increased Expression of p21WAF-1/CIP-1 in the Lens Epithelium of Rat Sugar Cataract

Takamura, Yoshihiro; Kubo, Eri; Tsuzuki, Shosai; Yagi, Hideshi; Sato, Makoto; Akagi, Yoshio

doi:10.1006/exer.2001.1120

Cited by 6 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could suggest a difference between inherited and diabetic cataract formation or simply that cataracts that develop multiple layers of LECs and plaques have signs of excessive dysregulated cellular proliferation where the cell cycle is not inhibited. Strong expression of PCNA, a commonly used indicator of cell proliferation, was found in the multilayered LECs of the rat sugar cataract along with an increase in p21 expression [42], as was seen in our study as well. p21 inhibits DNA replication by direct binding to PCNA, and increased expression of both suggests a dysregulation of p21 [42,46].…”

Section: Discussionsupporting

confidence: 62%

“…Strong expression of PCNA, a commonly used indicator of cell proliferation, was found in the multilayered LECs of the rat sugar cataract along with an increase in p21 expression [42], as was seen in our study as well. p21 inhibits DNA replication by direct binding to PCNA, and increased expression of both suggests a dysregulation of p21 [42,46].…”

Section: Discussionsupporting

confidence: 62%

“…p21 is a negative regulator of cell-cycle progression that mediates p53-induced cell-cycle arrest [13,42]; it has been shown to control the abnormal proliferation in experimental diabetic (sugar) cataract in the rat [21]. Though p21 has been studied in sugar cataract, it has not been investigated in any other type of cataract.…”

Section: Discussionmentioning

confidence: 98%

“…The well-balanced control of progression and inhibition of the cell cycle is essential to maintain the transparency of the lens [42]. p21 is a negative regulator of cell-cycle progression that mediates p53-induced cell-cycle arrest [13,42]; it has been shown to control the abnormal proliferation in experimental diabetic (sugar) cataract in the rat [21].…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of advanced glycation end-products in diabetic and inherited canine cataracts

Bras

Colitz

Kusewitt

et al. 2006

Graefe's Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

RAGE and PCNA expression did not increase with cataractogenesis, possibly due to overexpression associated with normal aging and constant exposure to oxidative stress from sunlight-related ultraviolet irradiation, respectively. However, p21 and PCNA increased in diabetic cataractogenesis suggesting cell cycle and proliferation dysregulation. This may be related to the rapid onset in this type of cataract compared with the more chronic and slower-to-develop inherited cataracts.

show abstract

Section: Discussionsupporting

confidence: 62%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of advanced glycation end-products in diabetic and inherited canine cataracts

Bras

Colitz

Kusewitt

et al. 2006

Graefe's Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

show abstract

“…Inhibitors of aldose reductase, which is involved in the polyol pathway, inhibit apoptosis, suggesting that sugar alcohol accumulation and apoptosis could be closely related [ 9 ]. In addition, expression of p21, which is involved in inhibition of cell proliferation, also increases as galactose-induced cataracts progress [ 11 ]. The balance between cell cycle progression and inhibition is important for maintenance of lens transparency.…”

Section: Introductionmentioning

confidence: 99%

Histone acetyltransferase inhibition reverses opacity in rat galactose-induced cataract

et al. 2022

Self Cite

View full text Add to dashboard Cite

Cataract, a disease that causes opacity of the lens, is the leading cause of blindness worldwide. Cataracts secondary to diabetes are common, even in young patients, so they are of significant clinical importance. Here, we used an ex vivo model of galactose-induced cataracts in the rat lens to investigate the therapeutic effects of histone acetyltransferase (HAT) inhibitors. Among the tested HAT inhibitors, TH1834 was the only one that could reverse most of the opacity once it had formed in the lens. Combination treatment with C646/CPTH2 and CBP30/CPTH2 also had therapeutic effects. In lens cross-sections, vacuoles were present in the tissue of the cortical equatorial region of untreated cataract samples. In treated cataract samples, lens tissue regenerated to fill the vacuoles. To identify the genes regulated by HAT inhibitors, qRT-PCR was performed on treated and untreated cataract samples to determine candidate genes. Expression of Acta1 and Stmn4, both of which are involved in the cytoskeleton, were altered significantly in C646+CPTH2 samples. Expression of Emd, a nuclear membrane protein, and Prtfdc1, which is involved in cancer cell proliferation, were altered significantly in CBP30+CPTH2 samples. Acta1, Acta2, Arrdc3, Hebp2, Hist2h2ab, Pmf1, Ppdpf, Rbm3, RGD1561694, Slc16a6, Slfn13, Tagln, Tgfb1i1, and Tuba1c in TH1834 samples were significantly altered. These genes were primarily related to regulation of cell proliferation, the cytoskeleton, and cell differentiation. Expression levels increased with the onset of cataracts and was suppressed in samples treated with HAT inhibitors.

show abstract

Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21cip1 expression

Cui

Wan

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

View full text Add to dashboard Cite

Increased Expression of p21WAF-1/CIP-1 in the Lens Epithelium of Rat Sugar Cataract

Cited by 6 publications

References 26 publications

Evaluation of advanced glycation end-products in diabetic and inherited canine cataracts

Evaluation of advanced glycation end-products in diabetic and inherited canine cataracts

Histone acetyltransferase inhibition reverses opacity in rat galactose-induced cataract

Deficiency of heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21cip1 expression

Contact Info

Product

Resources

About