Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer

Zhou, Hu; Wang, Fu-Bing; Chen, Hua; Tan, Qian; Qiu, Shili; Chen, Shanshan; Jing, Wei; Yu, Mingxia; Liang, Chunzi; Ye, Shengwei; Tu, Jiancheng

doi:10.18632/aging.101048

Cited by 75 publications

(53 citation statements)

References 47 publications

(53 reference statements)

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“…[6][7][8] For example, lncRNA LOC100129148 and EWSAT1 have been reported to act as oncogenic role in nasopharyngeal carcinoma, 9,10 lncRNA 00858 functions as a competing endogenous RNA for miR-422a to facilitate the cell growth in non-small cell lung cancer 11 and Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer. 12 It also has been reported that high DLX6-AS1 expression levels were significantly associated with both histological differentiation and TNM stage of lung cancer. Down-regulation of lncRNA DLX6-AS1 expression decreased the DLX6 mRNA and protein levels.…”

Section: Introductionmentioning

confidence: 92%

Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis

Zeng

et al. 2017

Cell Cycle

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Recently, long non-coding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in several types of cancer, including renal cell carcinoma (RCC). In this study, we identified an upregulated lncRNA, DLX6-AS1, in RCC tumor tissues compared with normal kidney tissues. Our data suggested that DLX6-AS1 promoted RCC cell growth and tumorigenesis via targeting miR-26a. In addition, we observed that PTEN overexpression restored the renal cancer cell growth and also rescued the RCC tumorigenesis. In summary, we conclude that DLX6-AS1 promotes renal cell carcinoma development via regulation of miR-26a/PTEN axis.

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Section: Introductionmentioning

confidence: 92%

Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis

Zeng

et al. 2017

Cell Cycle

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“…Recently, accumulating data demonstrated that non-coding RNAs (ncRNAs) are key regulators in both normal physiological and pathological conditions [5][6][7]. LncRNAs which are defined as those longer than~200 nucleotides, have been extensively characterized and it is evident that they are taking crucial roles in pathologies, including cancers [8][9][10][11][12][13]. In our previous study (GEO, http://www.ncbi.nlm.nih.…”

Section: Introductionmentioning

confidence: 99%

Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma

et al. 2017

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Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy. Long non‐coding RNAs (lncRNAs) are important regulators in pathological processes, yet their potential roles in PDAC are poorly understood. Here, we identify a fundamental role for a novel lincRNA, linc00511, in the progression of PDAC. Linc00511 levels in PDAC tissue specimens and cell lines were examined by quantitative real‐time PCR. Corresponding adjacent non‐neoplastic tissues were used as controls. The function of linc00511 in PDAC cell lines was determined by RNA interference approach in vitro and in vivo. Fluorescence in situ hybridization (FISH) was used to characterize linc00511 expression in PDAC cells. Insights of the mechanism of competitive endogenous RNAs (ceRNAs) were obtained from bioinformatic analysis, luciferase assays and RIP assays. The association between the linc00511/hsa‐miR29b‐3p axis and VEGFA was verified by Western blotting assay. Immunohistochemistry was performed to evaluate the expression of VEGFA in PDAC samples. The aberrant up‐regulation of linc00511 was detected in PDAC cell lines and patient specimens compared with controls. An increase in linc00511 expression indicates the adverse clinical pathological characteristics and poor prognosis. Functionally, linc00511 depletion in PDAC cells decreased proliferation, migration, invasion and endothelial tube formation. Mechanistically, linc00511 could up‐regulate VEGFA via its competing endogenous RNA (ceRNA) activity on hsa‐miR‐29b‐3p. In summary, our results define an important axis controlling proliferation, invasion and tumour angiogenesis in PDAC. Linc00511 is a novel lncRNA that plays a significant regulatory role in the pathogenesis and progression of PDAC. Thus, Linc00511 represents a new prognostic biomarker to predict clinical outcome of PDAC patients after surgery and may serve as a potential therapeutic target for PDAC treatment.

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“…15 Another study by Li, et al 16 also demonstrated the dysregulation of ln-cRNAs, which markedly mediated the progression of NPC, because ln-cRNA-ROR largely promoted proliferation and metastasis through the suppression of the p53 signalling pathway. ZFAS1 is a newly identified lncRNA that is up-regulated in several human cancers, such as gastric cancer, 17 non-small cell lung cancer 18 and hepatocellular carcinoma. 19 Although the latest encouraging research has reported that up-regulated ZFAS1 acts as a promoter for the occurrence of NPC through the activation of the Wnt/β-catenin pathway, the role of ZFAS1 in NPC requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

et al. 2019

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Objective: In this study, we explored the NPC-specific expression of ZFAS1 and the mechanism of ZFAS1-mediated growth, aggressiveness and tumorigenesis in NPC. Methods:The expression profile of lncRNAs was detected in NPC tissues and matching para-carcinoma tissues using microarray analysis. LncRNA-miRNA and miRNA-mRNA interaction networks were constructed using the miRcode v11 and TargetScanHuman v7.2 web server and then validated using dual-luciferase assay.Western blot and RT-qPCR were performed to detect protein and RNA expression.The effects of ZFAS1, miR-892b and LPAR1 dysregulation on the proliferative, migratory and invasive abilities of NPC cells were observed using colony formation, cell counting kit-8 (CCK-8) and transwell assays in vitro. In vivo, a xenograft nude mouse model was established to detect the impact of ZFAS1 dysregulation on the tumorigenicity of NPC cells. Results:The expression of multiple lncRNAs, of which ZFAS1 was up-regulated, was dysregulated in NPC tissues. ZFAS1 directly targeted miR-892b, and miR-892b negatively regulated the expression of downstream LPAR1. The proliferation, migration and invasion of NPC cells could be largely enhanced by the downregulation of miR-892b as well as the up-regulation of ZFAS1 and LPAR1, while the overexpression of miR-892b and the downregulation of ZFAS1 and LPAR1 decreased these abilities.In nude mice, the growth of tumour xenografts formed by HONE1 cells was significantly suppressed when ZFAS1 was silenced. Conclusion:The study demonstrated that lncRNA ZFAS1 may act as a promoter of tumorigenesis and metastasis in nasopharyngeal carcinoma, by up-regulating the expression of LPAR1 in a miR-892b-dependent manner. K E Y W O R D Slong noncoding RNAs, LPAR1, miR-892b, nasopharyngeal carcinoma, ZFAS1

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Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial-mesenchymal transition of gastric cancer

Cited by 75 publications

References 47 publications

Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis

Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis

Linc00511 acts as a competing endogenous RNA to regulate VEGFA expression through sponging hsa-miR-29b-3p in pancreatic ductal adenocarcinoma

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

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