Increased expression of IL-10 and IL-12 (p40) mRNA in Helicobacter pylori infected gastric mucosa: relation to bacterial cag status and peptic ulceration.

Abstract

Aims-To investigate interleukin (IL)-12(p40) and IL-10 mRNA expression levels in the gastric mucosa in relation to H pylori cag status, peptic ulceration, and histopathology. Methods-In 81 dyspeptic patients, antral and corpus biopsies were taken for reverse transcriptase polymerase chain reaction (RT-PCR) and histology. G3PDH (control) and IL-10 and IL-12 were coamplified in a duplex PCR and the ratios of cytokines to G3PDH were determined. Bacterial ureA and cagA status was determined by RT-PCR. (J Clin Pathol 1999;52:658-664) Results-IL-10

Lymphocyte Apoptosis, Proliferation and Cytokine Synthesis Pattern in Children with Helicobacter pylori Infection

“…These results are consistent with previous findings, indicating that H. pylori infection is related to a high expression of IL-10 in the gastric mucosa both on mRNA and protein level (Bodger et al, 2001;Hida et al, 1999). IL-10 has long been considered a potent anti-inflammatory cytokine strongly implicated in H. pylori infection (Bodger et al, 2001).…”

Exposure To Helicobacter Pylori Infection In Early Childhood And The Risk Of Allergic Disease And Atopic Sensitization: A Longitudinal Birth Cohort Study

“…Studies have shown that the H. pylori-induced inflammatory response is associated with Th1-mediated cellular responses [28,40], with higher expression of interferon-c (IFN-c) [40], IL-10, and IL-12 [41]. Particularly strong effects were seen in those possessing the pathogenic CagA + strains [28,41].…”

cDNA Array Analysis ofcagPathogenicity Island-AssociatedHelicobacter pyloriEpithelial Cell Response Genes

“…Total RNA was extracted from endoscopic gastric biopsies using Catrimox-14 (VH Bio Ltd.) and was treated with DNase I (Gibco Life Technologies). Purified RNA was reverse transcribed in 20 l of solution using 0.5 g of Random Primers (Promega) as previously described (28,62). Expression of genes of interest was examined by semiquantitative RT-PCR using primer sequences described in Table 1.…”