Increased Expression of <i>UBE2T</i> Predicting Poor Survival of Epithelial Ovarian Cancer: Based on Comprehensive Analysis of <i>UBE2s</i>, Clinical Samples, and the GEO Database

Zou, Ruoyao; Xu, Haoya; Li, Feifei; Wang, Shengke; Zhu, Li

doi:10.1089/dna.2020.5823

Cited by 20 publications

(13 citation statements)

References 67 publications

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“…Ubiquitin-conjugating enzyme E2A (UBE2A), which contains a 456-bp open reading frame, was predicted as a downstream target of miR-527 using bioinformatics tools, luciferase activity assays, qRT-PCR, and western blotting. It has been reported that increased UBE2A expression predicts poor survival in patients with ovarian cancer [ 44 ]. In addition, an RNA-seq signature involving UBE2A can predict the prognosis of multiple myeloma (MM) [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis

Nie

Peng

et al. 2022

Cell Death Discov.

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Circular RNAs (circRNAs) have been recognized as significant participants in the progression of different cancers; however, the detailed mechanisms of circRNAs in hepatocellular carcinoma (HCC) remain unclear. In this study, hsa_circ_0001394 was identified by RNA-seq analysis, and hsa_circ_0001394 was determined to be highly expressed in HCC specimens and cell lines. Patients with high expression of hsa_circ_0001394 tended to exhibit poor survival. Increased hsa_circ_0001394 expression in plasma was closely correlated with clinicopathological features including elevated vascular invasion and an advanced TNM stage, as indicated by alpha-fetoprotein (AFP) analysis. Hsa_circ_0001394 promoted the proliferation, migration, and invasion of HCC cells, whereas knockdown of hsa_circ_0001394 inhibited HCC tumorigenesis in vivo. In addition, mechanistic studies showed that miR-527 negatively interacted with hsa_circ_0001394. Furthermore, UBE2A was revealed to serve as a target of miR-527. Overall, the present study suggested that hsa_circ_0001394 may function as a sponge to promote HCC progression by regulating the miR-527/UBE2A pathway. Thus, hsa_circ_0001394 may become a promising biomarker and potential therapeutic target in HCC treatment.

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Section: Discussionmentioning

confidence: 99%

The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis

Nie

Peng

et al. 2022

Cell Death Discov.

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“…UBE2T (also known as FANCT ) expression appears to be a genetic marker distinguishing LMP from HGSOC ( Table 2 ). It has been reported that increased expression of UBE2T is associated with poor survival in EOC [ 12 ]. Machida et al (2006) identified UBE2T as a ubiquitin conjugase essential in the Fanconi anemia pathway (also known as the Fanconi anemia/BRCA pathway) and as a protein that is important in protecting chromosome stability [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…UBE2T has now been well characterized as a gene coding for a ubiquitin conjugase involved in the DNA damage response associated with the Fanconi anemia (FA) pathway [ 13 , 14 ]. UBE2T overexpression is associated with several cancers including prostate cancer [ 15 ], gastric cancer, [ 16 ], breast cancer [ 17 ], EOC [ 12 ], and multiple myeloma [ 18 ]. The role of UBE2T as an E2 conjugase in the FA pathway is to transfer ubiquitin from UBE2T to the E3 ligase FANCL, which, in turn, ubiquitinates FANCD2 ( Figure 2 ) [ 13 , 19 , 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

Ubiquitin Proteasome Pathway Transcriptome in Epithelial Ovarian Cancer

Vriend

Nachtigal

2021

Cancers

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In this article, we reviewed the transcription of genes coding for components of the ubiquitin proteasome pathway in publicly available datasets of epithelial ovarian cancer (EOC). KEGG analysis was used to identify the major pathways distinguishing EOC of low malignant potential (LMP) from invasive high-grade serous ovarian carcinomas (HGSOC), and to identify the components of the ubiquitin proteasome system that contributed to these pathways. We identified elevated transcription of several genes encoding ubiquitin conjugases associated with HGSOC. Fifty-eight genes coding for ubiquitin ligases and more than 100 genes encoding ubiquitin ligase adaptors that were differentially expressed between LMP and HGSOC were also identified. Many differentially expressed genes encoding E3 ligase adaptors were Cullin Ring Ligase (CRL) adaptors, and 64 of them belonged to the Cullin 4 DCX/DWD family of CRLs. The data suggest that CRLs play a role in HGSOC and that some of these proteins may be novel therapeutic targets. Differential expression of genes encoding deubiquitinases and proteasome subunits was also noted.

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“…Interest in nding useful biomarkers for diagnosis and treatment of breast cancer has been accumulating. Increasing data indicated that aberrant UBE2T expression has serve important roles in almost all cell biological behaviors, including growth, cell cycle, apoptosis, differentiation, apoptosis and metastasis (14)(15)(16)(17). However, little is known about the function of UBE2T in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

UBE2T knockdown inhibits cell growth and metastasis of breast cancer through Wnt/β-catenin pathway

Xueqin¹,

Mei²,

Yuping³

2021

Preprint

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Background: Emerging evidences have demonstrated that Ubiquitin-conjugating enzyme E2T (UBE2T) is dysregulated and play critical roles in various cancers. With the development of sequencing technology, studies have discovered that UBE2T is overexpressed in breast cancer tissues. However, the biological roles of UBE2T in breast cancer are still far to clear. In the present study, Methods: We analyzed the UBE2T expression in the Cancer Genome Atlas (TCGA) database. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to assess the expression of UBE2T in breast cancer cell lines. Cell proliferation, invasion and epithelial-mesenchymal transition (EMT) were determined by using CCK-8, EdU, Transwell and western blot assays.Results: UBE2T was highly expressed in breast cancer cell lines. Functional analysis revealed that silence or elevation of UBE2T inhibited or promoted the proliferation, migration, invasion and EMT and Wnt/β-catenin signaling pathway related markers of MCF-7 cells. Mechanically, blocking of Wnt/β-catenin pathway by XAV939 reversed the promotion effect of UBE2T overexpression on breast cancer cells’ proliferation, migration and invasion.Conclusion: Our findings emphasized that UBE2T may act as an oncogene via activating the Wnt/β-catenin pathway, which may provide a potential therapeutic target for the treatment of breast cancer.

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Increased Expression of UBE2T Predicting Poor Survival of Epithelial Ovarian Cancer: Based on Comprehensive Analysis of UBE2s, Clinical Samples, and the GEO Database

Cited by 20 publications

References 67 publications

The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis

The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis

Ubiquitin Proteasome Pathway Transcriptome in Epithelial Ovarian Cancer

UBE2T knockdown inhibits cell growth and metastasis of breast cancer through Wnt/β-catenin pathway

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