Increased expression of PRKCB mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

Abstract

The polymorphism of PRKCB has been proven to be associated with systemic lupus erythematosus (SLE) in our previous study. We aimed to investigate the relationship between expression of PRKCB mRNA and the Disease Activity Index (SLEDAI) and manifestations of SLE. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was applied to examine the expression of PRKCB mRNA in peripheral blood mononuclear cells of 60 patients with SLE and 62 controls. The Sequenom MassArray System was used to detect genotype SNP rs16972959. The expression levels of PRKCB mRNA in SLE cases were significantly increased compared with those in healthy controls (P < 0.001). In addition, PRKCB mRNA expression levels were negatively correlated with the SLEDAI (P < 0.05, r = -0.322), with lower mRNA expression levels of PRKCB in patients found with higher SLEDAI, presence of a new rash (P < 0.01), and proteinuria (P < 0.05). No association evidence was observed between the genotype of the variant rs16972959 and PRKCB mRNA expression levels; however, SNP rs16972959 was found to be an expression quantitative trait loci for PRKCB with the SLE risk allele correlated with increased expression in naïve monocytes (P = 9.12 × 10 ) and stimulated monocytes (9.24 × 10 > P > 2.75 × 10 ). On the other hand, SNP rs16972959 of PRKCB was found to have suggestive significant associations with vasculitis (P = 0.00718) of SLE. These results indicated that expression of PRKCB mRNA may be correlated with the pathogenesis of SLE; however, more investigation is still needed.

Filtration of Active Components with Antioxidant Activity Based on the Differing Antioxidant Abilities of Schisandrae Sphenantherae Fructus and Schisandrae Chinensis Fructus through UPLC/MS Coupling with Network Pharmacology

“…PRKCB (protein kinase C) has been identified as a drug treatment target for specific diseases involved in various cellular processes, such as the regulation of B-cell receptor (BCR) signaling bodies, oxidative stress-induced apoptosis, male hormone receptor-dependent transcriptional regulation, insulin signaling, and endothelial cell proliferation. [32], was observed to have upregulated mRNA expression in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus. MAPK8 and HIF1A were important targets in choline metabolism.…”

Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying

“…The PRKCB gene, which encodes the PKC protein, has been identified as a differential gene in transcriptome data. In various biological processes, including human systemic lupus erythematosus, the regulation of autophagy, and gastric cancer, this gene plays a crucial role [40][41][42].…”

Identification of significant genes with a poor prognosis in skin cutaneous malignant melanoma based on a bioinformatics analysis

“…PRKCB (also known as protein kinase C beta) is a serineand threonine-specific protein kinase family that can be activated by second messenger diacylglycerol and calcium. PRKCB family members are known to be involved in a variety of cellular signaling pathways and act as major receptors for phorbol esters (tumor promoters) (22,23). A wide variety of protein targets are phosphorylated by PRKCB family members.…”