Increased expression of HLA‐DR and CD86 in nasal epithelial cells in allergic rhinitics: antigen presentation to T cells and up‐regulation by diesel exhaust particles

Takizawa, Ryuta; Pawankar, Ruby; Yamagishi, Shigeki; Takenaka, Hiroshi; Yagi, Toshiaki

doi:10.1111/j.1365-2222.2007.02672.x

Cited by 27 publications

(23 citation statements)

References 60 publications

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“…12,13 It has also been demonstrated that increased transepithelial diffusion of large molecules occurs in epithelium absent of apical tight junctions, supporting a mechanism for more efficient antigen presentation. Subsequent in vitro studies have shown a mixed cytokine challenge (IFN-γ and TNF-α) promotes upregulation of major histocompatibility complex class II on cultured nasal epithelial cells, 14 suggesting that epithelial cells themselves may function as antigen-presenting cells in nasal mucosa.…”

Section: Discussionmentioning

confidence: 98%

Epithelial tight junction alterations in nasal polyposis

Rogers

Beste

Parkos

et al. 2011

Int Forum Allergy Rhinol

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Nasal polyposis is associated with epithelial TJP alterations. Further, the expression of TJPs in a model of inflamed respiratory mucosa is reduced in a similar fashion. Research on the histopathology of other epithelial inflammatory disorders suggests TJP alterations contribute to a self-perpetuating inflammatory state. Findings of this preliminary study support a similar process in nasal polyposis.

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Section: Discussionmentioning

confidence: 98%

Epithelial tight junction alterations in nasal polyposis

Rogers

Beste

Parkos

et al. 2011

Int Forum Allergy Rhinol

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“…However, HLA-DR has also been detected in other types of human cell, such as sinus epithelial cells, where its overexpression has been shown in chronic rhinosinusitis 2 and allergic rhinitis. 31 In fact, there is increasing evidence for the role of HLA-DR as a marker in systemic inflammatory disorders. As an example, in postoperative infectious complications, HLA-DR expression appears to be downregulated in immunocompetent cells, albeit without any predictive correlation.…”

Section: Discussionmentioning

confidence: 99%

Hyperosmolar Stress Upregulates HLA-DR Expression in Human Conjunctival Epithelium in Dry Eye Patients and In Vitro Models

Versura

Profazio

Schiavi

et al. 2011

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the immune response of human conjunctival epithelium to hyperosmolar stress. METHODS. Tear osmolarity was measured in 15 normal subjects and 25 dry eye (DE) patients; conjunctival imprint cytology samples were obtained at the nasal bulbar area. Subconfluent primary human conjunctival epithelial cells (pHCECs) and human conjunctival organ cultures (hCOCs), both cultured in iso-osmolar medium (305 mOsm/L), were exposed for 24 hours to media with progressively higher osmolarity, with or without the ion channel inhibitor ruthenium red (RuR). Human leukocyte antigen (HLA)-DR expression was evaluated by immunocytochemistry, on imprints from subjects, on primary human conjunctival epithelial cells, on formalin fixed-paraffin embedded hCOCs, and by RT-PCR. Statistical evaluation was performed by applying the unpaired Student's t test, as well as Spearman's rho and Pearson's r correlation coefficients (significance P Ͻ 0.05). RESULTS. HLA-DR expression increased in DE subjects with respect to control (% mean Ϯ SD, respectively, 46.16 Ϯ 7.2 vs. 7.48 Ϯ 1.14, P Ͻ 0.0001) and exhibited significantly high correlations with tear osmolarity values (r ϭ 0.614; P Ͻ 0.0001). In vitro experiments showed a progressive increase in HLA-DR expression as the osmolarity of the medium was increased from 6.75 Ϯ 1.16 (% mean Ϯ SD) in iso-osmolarcultured cells to 9.96 Ϯ 1.37 and 12.94 Ϯ 4.04 in cells cultured in, respectively, 350 and 400 mOsm/L (P Ͻ 0.05). A stepwise progressive increase was also found in hCOCs. Results were confirmed by RT-PCR. Ruthenium red significantly reduced HLA-DR expression in hyperosmolar-cultured cells. CONCLUSIONS. Data from complementary techniques demonstrate that extracellular hyperosmolarity induces HLA-DR overexpression in human conjunctival epithelial cells in both DE patients and in vitro cell culture models. (Invest Ophthalmol Vis Sci. 2011;52:5488 -5496)

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“…Recent studies demonstrated that nasal epithelial cells had a much wider range of activities, including release of cytokines and chemokines in human nasal epithelial cells (Kenney et al 1994;DaVern et al 1999;Nonaka et al 1996), which contributed to the enhancement of allergic reactions and presentation of antigens to T cells through increased expression of HLA-DR and CD86 molecules in patients with AR (Takizawa et al 2007). However, the performances of these cellular functions such as secretion, degranulation and proliferation are dependent on Ca 2+ signaling process.…”

Section: Discussionmentioning

confidence: 97%

Up-regulation of Orai1 in murine allergic rhinitis

Lin

Zheng

Zhang

et al. 2010

Histochem Cell Biol

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Orai1 is an essential pore-forming subunit of the Ca(2+) release-activated Ca(2+) channels and plays a key role in the store-operated Ca(2+) entry. However, little is known about the function of this pathway in allergic airway diseases. In this study, we evaluated Orai1 expression in normal and allergic rhinitis (AR) mice airway and spleen. AR models were established by repetitive intraperitoneal sensitization followed by intranasal challenge with ovalbumin. Sneezing was counted, and eosinophils infiltration was analyzed through Luna stain. We performed the analysis of Orai1 protein in airway and spleen by immunohistochemical staining, Western blotting and enzyme-linked immunosorbent assay, and quantitatively examined Orai1 mRNA in the above tissues by real-time reverse transcription-polymerase chain reaction. Sneezes and eosinophil counts in the AR group were increased in comparison to those in the normal group. Orai1 protein was expressed in mucosal epithelium and submucosal glands epithelium of airway, and in immune cells of spleen. The immunostaining appeared stronger in AR mice than that in normal ones. Both the Orai1 protein and mRNA levels showed up-regulation in the AR group compared with those in the normal one. Our results indicate that Orai1 is up-regulated in the airway and spleen in allergic inflammation and may participate in the pathogenesis of allergic rhinitis.

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Increased expression of HLA‐DR and CD86 in nasal epithelial cells in allergic rhinitics: antigen presentation to T cells and up‐regulation by diesel exhaust particles

Abstract: These studies suggest that NEC in patients with AR may play a role in antigen presentation through the enhanced expression of HLA-DR and CD86. Furthermore, these results suggest the possibility that DEP may enhance the antigen-presenting function of CNEC.

Cited by 27 publications

References 60 publications

Epithelial tight junction alterations in nasal polyposis

Epithelial tight junction alterations in nasal polyposis

Hyperosmolar Stress Upregulates HLA-DR Expression in Human Conjunctival Epithelium in Dry Eye Patients and In Vitro Models

Up-regulation of Orai1 in murine allergic rhinitis

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