Increased expression of heat shock protein 70 in chronic obstructive pulmonary disease

Dong, Jiajia; Guo, Lingli; Liao, Zenglin; Zhang, Mingke; Zhang, Mingzhou; Wang, Tao; Chen, Lei; Xu, Dan; Feng, Yu; Wen, Fuqiang

doi:10.1016/j.intimp.2013.09.003

Cited by 35 publications

(43 citation statements)

References 39 publications

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“…In this respect, there are several possible factors that may produce the cancer selectivity of SBL by affecting the internalization or translocation of SBL. The members of the Hsp family form a complex and interact with some receptors such as epidermal growth factor receptor (31, 32) or rota virus receptor (integrin αvβ3) (33) and regulate receptor including ligand binding and penetration. However, reducing Hsp70 expression does not affect the binding of SBL but instead attenuates its apoptosis-inducing effects.…”

Section: Cancer Specificity (Predicted Possibility) and Factors That mentioning

confidence: 99%

Cancer-Selective Induction of Apoptosis by Leczyme

et al. 2014

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Sialic acid-binding lectin (SBL) is a multi-functional protein that is isolated from oocytes of Rana catesbeiana. It has both lectin and ribonuclease (enzyme) properties, and therefore is called leczyme. We examined the anti-tumor effects of SBL and discovered that SBL has potential as a new type of anti-cancer drug. SBL causes a cancer-selective induction of apoptosis by multiple signaling pathways whereby RNA is its target. It is suggested that the mitochondrial pathway and endoplasmic reticulum stress-mediated pathway participate in SBL-induced signaling. The synergistic anti-tumor effects with other molecules, such as tumor necrosis factor-related apoptosis ligand and interferon γ, have been reported. In this study, we summarize the effects of SBL and focus on its cancer-selective apoptotic properties. In addition, we present a possible explanation for its cancer specificity.

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Section: Cancer Specificity (Predicted Possibility) and Factors That mentioning

confidence: 99%

Cancer-Selective Induction of Apoptosis by Leczyme

et al. 2014

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“…Chronic obstructive pulmonary disease is characterized by irreversible chronic airflow limitation that is the consequence of defective tissue repair and chronic inflammation, leading to parenchymal destruction and small airway fibrosis . In addition to the different types of cellular reaction, many inflammatory mediators such as reactive oxygen and nitrogen species, interleukin (IL)‐8, tumour necrosis factor (TNF)‐α, IL‐6 and interferon (IFN)‐γ are involved in the process of inflammation in COPD . Danger signals, such as pathogen‐associated molecular patterns (PAMPs) and damage‐associated molecular patterns (DAMPs), activate families of pattern recognition receptors (PRRs) that include the Toll‐like receptors (TLRs) .…”

Section: Introductionmentioning

confidence: 99%

Pro‐inflammatory effects of extracellular Hsp70 on NCI‐H292 human bronchial epithelial cell line

Hulina

Rajković

Jelić³

et al. 2019

Int J Experimental Path

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Summary Extracellular Hsp70 (eHsp70) exerts its biological actions via Toll‐like receptors 2 and 4, and is increased in sera of chronic obstructive pulmonary disease (COPD) patients. The aim of this study was to explore the pro‐inflammatory effects and cytotoxicity of eHsp70 alone and in combination with bacterial components lipoteichoic acid (LTA) and lipopolysaccharide (LPS) on NCI‐H292 airway epithelial cells. NCI‐H292 cells were treated with recombinant human Hsp70 protein (rhHsp70), LPS, LTA and their combinations for 4, 12, 24 and 48 hours. IL‐6, IL‐8 and TNF‐α levels were measured by an ELISA method. Cell viability was determined by the MTS method, and caspase‐3/7, caspase‐8 and caspase‐9 assays. rhHsp70 induced secretion of IL‐6 and IL‐8 in a concentration‐ and time‐dependent manner, with the highest secretion at 24 hours. rhHsp70 combined with LTA had antagonistic and with LPS synergistic effect on IL‐6 secretion, while the interactions between rhHsp70 and LPS or LTA on IL‐8 were synergistic. TNF‐α was not detected in the applied conditions. rhHsp70, LPS or LTA did not affect cell viability, and rhHsp70 even suppressed caspase‐3/7 activities. We suggest that pro‐inflammatory effects of eHsp70, together with other damaging molecules and/or COPD risk factors, might contribute to the aggravation of chronic inflammation in human bronchial epithelium.

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“…Previous studies indicated that altered levels of Hsps in serum and peripheral blood cells were associated with increased COPD risk in the general population (Hacker et al 2009;Jan Ankersmit et al 2012;Dong et al 2013). However, few similar studies have been conducted among coal workers.…”

Section: Introductionmentioning

confidence: 99%

COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study

Cui

Xing

Liu

et al. 2015

Cell Stress and Chaperones

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This case-control study aimed to investigate whether the levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes were associated with the risk of chronic obstructive pulmonary disease (COPD) among coal workers. A total of 76 COPD cases and 48 age-matched healthy controls from a group of coal workers were included. The case group consisted of 35 COPD patients whose condition was complicated with coal workers' pneumoconiosis (CWP) and 41 COPD patients without CWP. Heat shock proteins (Hsps) in plasma and lymphocytes were detected by ELISA and flow cytometry, respectively. Multiple logistic regression models were applied to estimate the association between Hsp levels and COPD risk. Our results showed that plasma Hsp70 and lymphocyte Hsp27 levels were significantly higher and plasma Hsp27 levels were significantly lower in COPD cases than in controls (p<0.01). No significant differences in lymphocyte Hsp70 levels were found between COPD cases and the matched subjects. Higher plasma Hsp70 levels (odds ratio (OR)=13.8, 95 % confidence interval (CI)=5.7-33.5) and lower plasma Hsp27 levels (OR=4.6, 95 % CI= 2.0-10.5) were significantly associated with an increased risk of COPD after adjusting for confounders. Higher lymphocyte Hsp27 levels were only associated with an increased risk of COPD with CWP (OR=6.6, 95 % CI=2.0-22.1) but not with an increased risk of COPD without CWP (OR=3.0, 95 % CI= 0.9-8.9). Additionally, there were strong joint effects of different Hsps on COPD risk. These results showed that higher levels of plasma Hsp70 and lower levels of plasma Hsp27 might be associated with an increased risk of COPD among coal workers. They may have the potential to serve as monitoring markers for COPD in coal workers.

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Increased expression of heat shock protein 70 in chronic obstructive pulmonary disease

Cited by 35 publications

References 39 publications

Cancer-Selective Induction of Apoptosis by Leczyme

Cancer-Selective Induction of Apoptosis by Leczyme

Pro‐inflammatory effects of extracellular Hsp70 on NCI‐H292 human bronchial epithelial cell line

COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study

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