Increased Expression of Gelatinase (MMP-2 and MMP-9) in Pterygia and Pterygium Fibroblasts with Disease Progression and Activation of Protein Kinase C

Yang, Shun‐Fa; Lin, Ching-Yang; Yang, Peiyu; Chao, Shih-Chun; Ye, Yi-Zhen; Hu, Dan‐Ning

doi:10.1167/iovs.08-3147

Cited by 70 publications

(57 citation statements)

References 34 publications

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“…Ocular dryness has therefore been proposed as a risk factor for pterygium and pterygium recurrence by triggering the secretion of potent proinflammatory cytokines and increasing the expression of MMPs by conjunctival epithelial cells [20] and pterygium body fibroblasts [21]. Indeed, levels of MMP-9 -the most important gelatinase on the ocular surface -are significantly elevated in DED [20] and have also been shown to correlate with pterygium formation and progression [22]. Importantly, VanDerMeid et.al.…”

Section: Discussionmentioning

confidence: 99%

Dry-Eye Disease in Recurrent Pterygium

Tan

Vollmer‐Conna

Tat³

et al. 2018

Ophthalmic Res

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Purpose: The aim of this paper was to investigate the degree of inflammation and dry-eye disease (DED) in recurrent pterygium. Methods: Fifty-five patients with a history of pterygium excision were divided into 3 groups – Group 1: no recurrence after the first excision; Group 2: recurrence after the first excision; Group 3: no recurrence after the second excision. Dry-eye symptoms and tear production were elicited for each patient. Results: A high prevalence of DED was observed in patients with primary and recurrent pterygium based on self-reported dry-eye symptoms (63.6%) and an abnormal Schirmer test result (78.1%). There was a statistically significant difference (p = 0.025) in the Schirmer test values between patients with (5.6 mm) and without (11.4 mm) recurrence after surgery for primary pterygium. Patients who were cured after a second excision had an improved Schirmer test value (9.5 mm), although this was not significant. Conclusion: Pterygium recurrence is associated with a greater severity of dry eye, possibly by perpetuating ocular surface inflammation in the postoperative period.

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Section: Discussionmentioning

confidence: 99%

Dry-Eye Disease in Recurrent Pterygium

Tan

Vollmer‐Conna

Tat³

et al. 2018

Ophthalmic Res

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“…A previous report showed that pterygium head Wbroblasts had higher expression and active MMP-1 and MMP-3 (Li et al 2001). All these MMPs (1, 2, 3 and 9) would then work synergistically to degrade the Bowman's membrane and ECM (Yang et al 2009). The invasion capacity was also regulated by their interaction with TIMPs (Tsai et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Proliferative and migratory aptitude in pterygium

Bai

Teng

Wong

et al. 2010

Histochem Cell Biol

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Pterygium is a chronic fibrovascular overgrowth on the corneal surface and is often associated with inflammation, astigmatism and obstructed vision. The common treatment is surgical removal but post-operative recurrences with more aggressive behavior are common. However, there is a controversy in the pathogenesis of primary pterygium between limbal stem cell failure versus proliferation. In this study, we explore the proliferative and migratory aptitude in pterygium by characterizing the growth and migration pattern of pterygial cells in the head (on the cornea), the neck (over the focal limbus), and the body (on the conjunctiva) epithelia of 12 full-length primary pterygia. Immunofluorescence and quantification analyses showed a spatial expression pattern of markers for stem cells, cell growth, and matrix metalloproteinases. Beside the basal epithelia in all three regions, p63α(strong) cells were located in suprabasal layers in head, weak in the body and absent in neck. Pertinent cell proliferation in head than body epithelia was revealed by its higher colony-forming efficiency. ATP-binding cassette transporter glycoprotein family member-2 and cytokeratin-15 were found mainly in the body basal epithelia, similar to that in normal conjunctiva. Much fewer proliferating stem-like cells in the neck region supported the limbal failure as a cause of pterygium formation. Pax6, matrix metalloproteinase-2 and -9 were more expressed in the head than in the other two regions. Our results indicate the importance of pterygium head in tissue growth and invasion and its likely involvement in post-operation recurrence.

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“…The results showed that the MMP-2 and MMP-9 expression levels in pterygium tissues and fibroblasts were higher than those of normal tissues and fibroblasts and closely relevant to the progression of pterygium. Also, in early-stage pterygium tissues and cultured fibroblasts, MMP-2 or MMP-9 was at low or undetectable levels, while in advanced-stage pterygium (pterygium head passed the papillary region), MMP-2 and MMP-9 could be obviously detected in pterygium tissues and fibroblasts (16). In addition, down-regulation of the expression levels of MMP-3 and MMP-13 was capable of suppression of the proliferation and migration of pterygium fibroblasts (24,25).…”

Section: Discussionmentioning

confidence: 94%

“…The results showed that MMP-1 and MMP-3, but not MMP-2, TIMP-1, TIMP-2 or uPA, were highly expressed in pterygium head, body and subconjunctival fibroblasts (14). Although Schellini and his colleagues demonstrated that the expression of MMP-9 was similar among the tissues from normal Tenon's capsule and primary or recurrent pterygia (15), Yang has oppositely proposed that the expression of MMP-9 by pterygium fibroblasts is significantly increased after the progression of pterygium (16). One of the reasons to explain the inconsistency is that the clinical sample sizes in the two researchers were only 6 and 15, respectively.…”

mentioning

confidence: 99%