Increased expression of antibiotic-resistance genes in biofilm communities upon exposure to cetyltrimethylammonium bromide (CTAB) and other stress conditions

Luo, Yi; Lai, Yen Jung Sean; Zheng, Chenwei; Ilhan, Zehra Esra; Ontiveros‐Valencia, Aura; Long, Xiangxing; Krajmalnik‐Brown, Rosa; Rittmann, Bruce E.

doi:10.1016/j.scitotenv.2020.144264

Cited by 19 publications

(9 citation statements)

References 70 publications

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“…59 Oxidative stress appeared to be a key factor in enhancing ARG expression to trigger ARG proliferation. 21 The significant co-occurrence patterns of ARGs and MGEs exposed to different BC concentrations in the network analysis provided further insights into the potential BC stress-induced coselection of ARGs. High BC concentrations in the network might apply adequate selective pressures on the co-occurrence of ARGs and MGEs, thus resulting in higher co-occurrence incidences.…”

Section: Bc-induced Coselection Of the Antibiotic Resistomementioning

confidence: 89%

“…This might be caused by the destruction of cell membranes by medium and high concentrations of BC, causing oxidative stress in bacteria . Oxidative stress appeared to be a key factor in enhancing ARG expression to trigger ARG proliferation …”

Section: Discussionmentioning

confidence: 99%

“…18 Thus, ARGs may develop horizontal gene transfer of QAC resistance genes, expanding the resistant flora, 19 especially under the background of compound pollution of sulfonamides and QACs. To date, studies based on freshwater microcosms, 20 membrane biofilm reactors, 21 and bacterial isolates 22 have shown that QAC exposure promotes microbial resistance to antibiotics. Nevertheless, few studies have focused on the changes in antibiotic resistance induced by QACs in the soil environment.…”

Section: Introductionmentioning

confidence: 99%

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Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Zeng

Jin

et al. 2022

Environ. Sci. Technol.

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Antibiotic resistance genes (ARGs) are global pollutants that pose a potential risk to human health. Benzalkonium chloride (C12) (BC) disinfectants are thought to exert selection pressure on antibiotic resistance. However, evidence of BC-induced changes in antibiotic resistance in the soil environment is lacking. Here, we established short-term soil microcosms to investigate ARG profile dynamics in agricultural soils amended with sulfamethazine (SMZ, 10 mg kg–1) and gradient concentrations of BC (0–100 mg kg–1), using high-throughput quantitative PCR and Illumina sequencing. With the increase in BC concentration, the number of ARGs detected in the soil increased, but the normalized ARG abundance decreased. The added SMZ had a limited impact on ARG profiles. Compared to broad-spectrum fungicidal BC, the specificity of SMZ significantly affected the microbial community. Network analysis found that low–medium BC exposure concentrations resulted in the formation of small but strong ARG co-occurrence clusters in the soil, while high BC exposure concentration led to a higher incidence of ARGs. Variation partitioning analysis suggested that BC stress was the major driver shaping the ARG profile. Overall, this study highlighted the emergence and spread of BC-induced ARGs, potentially leading to the antimicrobial resistance problem in agricultural soils.

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Section: Bc-induced Coselection Of the Antibiotic Resistomementioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Zeng

Jin

et al. 2022

Environ. Sci. Technol.

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“…However, WW treatment plants are also regarded as selection and dissemination hot spots due to the high density of human gut, environmental bacteria, and chemical pollutants (22)(23)(24). Biofilms in WW systems represent an important source of ARGs and bacteria that may end up circulating in the environment and hence present an important model to study the dissemination of resistance in the context of discharged effluents (4,25,26). Biofilms dominate most habitats on earth, accounting for ~ 80% of all bacterial and archaeal cells (27).…”

Section: Introductionmentioning

confidence: 99%

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

Buelow

Dauga

Carrion

et al. 2023

Preprint

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We studied hospital and urban wastewater (WW) biofilms in situ and in an in vitro laboratory model to assess and compare their potential to actively disseminate and express ARGs. Biofilms were subjected to confocal microscopy to qualify and quantify their extracellular polymeric substances (EPSs), including polysaccharides, external DNA (eDNA), and lipids. We observed that antibiotic challenge had a differential impact on the EPS and the live-dead ratios of in vitro hospital and urban WW biofilms. Differential expression of a range of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), including those on plasmids, was assessed using a meta-transcriptomic approach. Expression of ARGs as well as MGEs, in situ, was 10-fold higher in hospital WW biofilms compared with urban, suggesting a higher frequency of ARG transfer might occur in hospital WW. In vitro data confirmed that the addition of ciprofloxacin significantly increased ARG and MGE expression in hospital but not in urban WW biofilms suggesting that the regulation of ARG and MGE expression is likely dependent on the structure and composition of the biofilm. The different responses to antibiotic pressure in hospital vs urban biofilms, coupled with differences in biofilm structure helps delineate distinct characteristics of hospital and urban WW biofilms.

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“…However, WW treatment plants are also regarded as selection and dissemination hot spots due to the high density of human gut, environmental bacteria, and chemical pollutants [22][23][24]. Bio lms in WW systems represent an important source of ARGs and bacteria that may end up circulating in the environment and hence present an important model to study the dissemination of resistance in the context of discharged e uents [4,25,26]. Bio lms dominate most habitats on earth, accounting for ~ 80% of all bacterial and archaeal cells [27].…”

Section: Introductionmentioning

confidence: 99%

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

Buelow

Dauga

Carrion

et al. 2023

Preprint

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Background Demonstration of the transfer, dynamics, and regulation of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in a complex environmental matrix is yet experimentally challenging, with many essential open questions such as how and where transfer and dissemination of ARGs happens in nature. The extent and conditions of MGEs transfer that carry ARGs is still largely unexplored in natural environments and microbial communities. Biofilms are structures that include high density multi-species bacterial communities embedded in self-produced extracellular polymeric substances (EPS) constituting a matrix that facilitates gene transfer and where bacteria exhibit high tolerance to stress and to antibiotics. In this study we implemented a sampling and analysis approach that allows phenotypic and genomic analyses of in situ and reconstituted in vitro hospital and urban wastewater (WW) biofilms. To assess the potential of hospital and urban WW biofilms to efficiently disseminate ARGs in the WW system, we explored the EPS within the biofilm matrix and assessed the expression of the resistome (ARGs) and mobilome (MGEs) by metatranscriptomics. Results We first showed that a) the composition of EPS differs depending on their growth environment (in situ and in vitro) and their sampling origin (hospital vs urban WW) and that b) a low amount of ciprofloxacin impacted the composition of the EPS. Next, the metatranscriptomic approach showed that a) expression of ARGs and MGEs increase upon adding a low amount of ciprofloxacin for biofilms from hospital WW but not for those from urban WW and b) that expression of specific plasmids that carry individual or multiple ARGs varies depending on the WW origins of the biofilms. When the same plasmids were expressed in both, urban and hospital WW biofilms, they carried and expressed different ARGs. Conclusion We show that hospital and urban wastewaters shape the structure and active resistome of environmental biofilms, and we confirmed that hospital WW is an important hot spot for the dissemination and selection of AMR. The different responses to antibiotic pressure in hospital vs urban biofilms, coupled with differences in biofilm structure helps delineate distinct characteristics of hospital and urban WW biofilms highlighting the relationships between the resistome and its expression in environmental biofilms and their surrounding ecosystems.

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Increased expression of antibiotic-resistance genes in biofilm communities upon exposure to cetyltrimethylammonium bromide (CTAB) and other stress conditions

Cited by 19 publications

References 70 publications

Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

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Increased expression of antibiotic-resistance genes in biofilm communities upon exposure to cetyltrimethylammonium bromide (CTAB) and other stress conditions

Cited by 19 publications

References 70 publications

Short-Term Benzalkonium Chloride (C12) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Short-Term Benzalkonium Chloride (C12) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

Hospital and urban wastewaters shape the structure and active resistome of environmental biofilms

Contact Info

Product

Resources

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Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils

Short-Term Benzalkonium Chloride (C₁₂) Exposure Induced the Occurrence of Wide-Spectrum Antibiotic Resistance in Agricultural Soils