Increased expression levels of CD300c on basophils from allergic individuals

Vitallé, Joana; Terrén, Íñigo; Orrantia, Ane; Segurola, Aritza; Seras, Yolanda; Gamboa, P. M.; Borrego, Francisco; Zenarruzabeitia, Olatz

doi:10.1016/j.waojou.2019.100060

Cited by 6 publications

(10 citation statements)

References 12 publications

(16 reference statements)

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“…We did not observe significant differences in the number of blood basophils between patients with moderate-to-severe AD and healthy controls (Figure 2A). Our results showed that, compared to healthy individuals, basophils from moderate-tosevere AD children expressed significantly higher expression levels of FcεRI, and that the expression of the basophil activation marker CD63 also tended to be higher (Figure 2B), in accordance with previously reported results in basophils from allergic individuals (38). Human basophils express, among others, the chemokine receptors CCR2, which positively regulates basophil degranulation in response to monocyte chemoattractant protein-1 (MCP-1), and CXCR4, important for basophil activation and recruitment to inflammatory sites (51,52).…”

Section: Phenotypic Characterization Of Basophils Fromsupporting

confidence: 92%

“…The immune response is also regulated, among others, by activating and inhibitory receptors expressed on the surface of immune cells. In this sense, the involvement of CD300 surface receptors in the regulation of allergic responses has been demonstrated (34)(35)(36)(37)(38)(39). It is known that CD300a expressed on basophils suppresses the basophil anaphylactic degranulation by its interaction with phosphatidylserine and phosphatidylethanolamine exposed on apoptotic cells (34,35), whereas CD300c works as a costimulatory molecule during immunoglobulin E (IgE)-mediated basophils activation (37).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in basophils from allergic individuals, a lower expression of CD300a inhibitory receptor (34) and a higher expression of CD300c activating receptor (37,38) have been described. In AD patients a significant increase in CD300a total expression was observed in biopsies from lesional skin compared to normal skin, specifically on eosinophils, macrophages, and mast cells (40).…”

Section: Introductionmentioning

confidence: 99%

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Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

et al. 2021

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Background: Infants with severe atopic dermatitis (AD) may be sensitized to foods that have not been introduced into their diet, posing a risk for developing an immediate hypersensitivity reaction on the first exposure to the food to which they are sensitized. The aim of this work was to perform an analysis of the sensitization profile in infants with moderate-to-severe AD and to identify cellular and molecular markers for food allergy (FA).Methods: Blood samples from healthy donors and children with moderate-to-severe AD were studied. Specific IgE to several allergens were determined using ImmunoCAP FEIA system and ISAC technology. Furthermore, using flow cytometry-based studies, basophils and regulatory T (Treg) cells were phenotypically characterized.Results: 90% of children with AD were sensitized to food antigens before introducing them into the diet, and 100% developed FA. Phenotypic analysis showed a significantly higher percentage of CTLA-4 and PD-1 expressing Treg cells in AD patients than in healthy controls. Basophils from patients exhibited a marked reduction in the expression of CD300a, higher expression of FcεRI and CXCR4, and to some extent higher expression of CD63 and CD300c.Conclusions: Infants with moderate-to-severe AD are at high risk of being sensitized to food allergens. Therefore, to avoid allergic reactions, broad-spectrum sensitization studies are necessary before introducing complementary diet. Increased expression of CTLA-4 and PD-1 suggests greater suppressive potential of Treg cells in infants with AD than healthy controls. Furthermore, our results suggest a role for CD300 molecules on circulating basophils as possible biomarkers for FA susceptibility.

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Section: Phenotypic Characterization Of Basophils Fromsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

et al. 2021

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“…The contents released include histamine, tryptase, cysteinyl leukotrienes and prostaglandin D2 [43,47]. Notably, overexpression of CD300c, which acts in a co-stimulatory manner for IgE-dependent basophil activation, is seen in patients with AR [48].…”

Section: Early Phase Responsesmentioning

confidence: 99%

Mechanisms of Allergen Immunotherapy in Allergic Rhinitis

Drazdauskaité

Layhadi

Shamji

2020

Curr Allergy Asthma Rep

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Purpose of Review Allergic rhinitis (AR) is a chronic inflammatory immunoglobulin (Ig) E-mediated disease of the nasal mucosa that can be triggered by the inhalation of seasonal or perennial allergens. Typical symptoms include sneezing, rhinorrhea, nasal itching, nasal congestion and symptoms of allergic conjunctivitis. AR affects a quarter of the population in the United States of America and Europe. Recent Findings AR has been shown to reduce work productivity in 36–59% of the patients with 20% reporting deteriorated job attendance. Moreover, 42% of children with AR report reduced at-school productivity and lower grades. Most importantly, AR impacts the patient’s quality of life, due to sleep deprivation. However, a proportion of patients fails to respond to conventional medication and opts for the allergen immunotherapy (AIT), which currently is the only disease-modifying therapeutic option. AIT can be administered by either subcutaneous (SCIT) or sublingual (SLIT) route. Both routes of administration are safe, effective, and can lead to tolerance lasting years after treatment cessation. Both innate and adaptive immune responses that contribute to allergic inflammation are suppressed by AIT. Innate responses are ameliorated by reducing local mast cell, basophil, eosinophil, and circulating group 2 innate lymphoid cell frequencies which is accompanied by decreased basophil sensitivity. Induction of allergen-specific blocking antibodies, immunosuppressive cytokines, and regulatory T and B cell phenotypes are key pro-tolerogenic adaptive immune responses. Conclusion A comprehensive understanding of these mechanisms is necessary for optimal selection of AIT-responsive patients and monitoring treatment efficacy. Moreover, it could inspire novel and more efficient AIT approaches.

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“…Over the last few years, the biological and clinical significance of CD300 molecules and their participation in the pathogenesis of numerous diseases such as allergy, psoriasis, colitis, multiple sclerosis, leukemia, sepsis, infection diseases, etc. have been well documented [21,23,25,[52][53][54][55][56][57][58][59][60][61]. In this review, our main objective is to describe the current knowledge of the expression and function of CD300 molecules in key effector cells of allergic reactions, specifically mast cells, basophils and eosinophils (Table 1), which have an essential role in the effector phases of allergic responses.…”

Section: Cd300 Receptor Familymentioning

confidence: 99%

The Expression and Function of CD300 Molecules in the Main Players of Allergic Responses: Mast Cells, Basophils and Eosinophils

Vitallé

Terrén

Orrantia

et al. 2020

IJMS

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Allergy is the host immune response against non-infectious substances called allergens. The prevalence of allergic diseases is increasing worldwide. However, while some drugs counteract the symptomatology caused by allergic reactions, no completely effective treatments for allergic diseases have been developed yet. In this sense, the ability of surface activating and inhibitory receptors to modulate the function of the main effector cells of allergic responses makes these molecules potential pharmacological targets. The CD300 receptor family consists of members with activating and inhibitory capabilities mainly expressed on the surface of immune cells. Multiple studies in the last few years have highlighted the importance of CD300 molecules in several pathological conditions. This review summarizes the literature on CD300 receptor expression, regulation and function in mast cells, basophils and eosinophils, the main players of allergic responses. Moreover, we review the involvement of CD300 receptors in the pathogenesis of certain allergic diseases, as well as their prospective use as therapeutic targets for the treatment of IgE-dependent allergic responses.

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Increased expression levels of CD300c on basophils from allergic individuals

Cited by 6 publications

References 12 publications

Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

Mechanisms of Allergen Immunotherapy in Allergic Rhinitis

The Expression and Function of CD300 Molecules in the Main Players of Allergic Responses: Mast Cells, Basophils and Eosinophils

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