Increased expression and secretion of interleukin-6 in human parvovirus B19 non-structural protein (NS1) transfected COS-7 epithelial cells

Hsu, Tsai‐Ching; Tzang, Bor Show; Huang, Cheng‐Yi; Lee, Y.-J.; Liu, G.-Y.; Chen, M.-C.; Tsay, Gregory J.

doi:10.1111/j.1365-2249.2006.03023.x

Cited by 39 publications

(36 citation statements)

References 28 publications

(40 reference statements)

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“…Among the SLE patients with dilated cardiomyopathies, considerably raised levels of Th17-related cytokines were also observed in those with PVB19-NS1 antibody versus those without PVB19-NS1 antibody. These results were in agreement with the findings of previous studies demonstrating that PVB19 NS1 protein is a transactivator of IL-6 expression [99,100], indicating that NS1 may be important in the PVB19-related diseases.…”

Section: Systemic Lupus Erythematosus (Sle)supporting

confidence: 95%

Human parvovirus B19 and autoimmune diseases. Review of the literature and pathophysiological hypotheses

Page

François²,

Goëb

et al. 2015

Journal of Clinical Virology

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Section: Systemic Lupus Erythematosus (Sle)supporting

confidence: 95%

Human parvovirus B19 and autoimmune diseases. Review of the literature and pathophysiological hypotheses

Page

François²,

Goëb

et al. 2015

Journal of Clinical Virology

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“…Previous experiments using GFP to visualize NS1 PVB19 transfection in epithelial cells found an enhanced inflammatory response with a high expression and secretion of IL-6 [9]. Thus, NS1 PVB19 inflammatory signaling modulation activates signal transducers and activators of transcription 3 (STAT3) [10], which induce an enhanced ICAM1 expression [18].…”

Section: Discussionmentioning

confidence: 98%

Green Fluorescent Protein (GFP) Color Reporter Gene Visualizes Parvovirus B19 Non-Structural Segment 1 (NS1) Transfected Endothelial Modification

et al. 2012

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BackgroundHuman Parvovirus B19 (PVB19) has been associated with myocarditis putative due to endothelial infection. Whether PVB19 infects endothelial cells and causes a modification of endothelial function and inflammation and, thus, disturbance of microcirculation has not been elucidated and could not be visualized so far.Methods and FindingsTo examine the PVB19-induced endothelial modification, we used green fluorescent protein (GFP) color reporter gene in the non-structural segment 1 (NS1) of PVB19. NS1-GFP-PVB19 or GFP plasmid as control were transfected in an endothelial-like cell line (ECV304). The endothelial surface expression of intercellular-adhesion molecule-1 (CD54/ICAM-1) and extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) were evaluated by flow cytometry after NS-1-GFP or control-GFP transfection. To evaluate platelet adhesion on NS-1 transfected ECs, we performed a dynamic adhesion assay (flow chamber). NS-1 transfection causes endothelial activation and enhanced expression of ICAM-1 (CD54: mean±standard deviation: NS1-GFP vs. control-GFP: 85.3±11.2 vs. 61.6±8.1; P<0.05) and induces endothelial expression of EMMPRIN/CD147 (CD147: mean±SEM: NS1-GFP vs. control-GFP: 114±15.3 vs. 80±0.91; P<0.05) compared to control-GFP transfected cells. Dynamic adhesion assays showed that adhesion of platelets is significantly enhanced on NS1 transfected ECs when compared to control-GFP (P<0.05). The transfection of ECs was verified simultaneously through flow cytometry, immunofluorescence microscopy and polymerase chain reaction (PCR) analysis.ConclusionsGFP color reporter gene shows transfection of ECs and may help to visualize NS1-PVB19 induced endothelial activation and platelet adhesion as well as an enhanced monocyte adhesion directly, providing in vitro evidence of possible microcirculatory dysfunction in PVB19-induced myocarditis and, thus, myocardial tissue damage.

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“…It is shown that B19V proteins NS1 (Hsu et al , 2006; Kerr et al , 2001) and VP1u (Tzang et al , 2009a) induce IL-6 mRNA expression. B19V proteins during viraemia could induce IL-6 production in RA patients.…”

Section: Discussionmentioning

confidence: 99%

Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

Naciute

Mieliauskaitė

Rugienė

et al. 2016

Journal of General Virology

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The present study aims to clarify the possible involvement of parvovirus B19 (B19V) infection in rheumatoid arthritis (RA) pathogenesis by investigating the presence of B19V infection markers (genomic sequences and virus-specific antibodies) in association with the level of cytokines and RA clinical activity and aggressiveness. A total of 118 RA patients and 49 age- and sex-matched healthy volunteers were enrolled in the study. Nested PCR was used to detect B19V sequences in whole blood and cell-free plasma DNA, ELISA to detect virus-specific antibodies and cytokine levels in plasma and recomLine dot blot assay for antibodies to separate B19V antigens. The detection frequency of B19V DNA was higher in patients with RA (25.4 %) in comparison with healthy persons (18.4 %). B19V DNA in cell-free plasma (B19+p) was detected significantly often in RA patients in comparison with healthy controls (13.6 vs 2 %; P=0.0002). RA B19+p patients had higher disease activity and aggressiveness, decreased haemoglobin and increased erythrocyte sedimentation rates. IL-6 plasma levels were significantly higher in RA patients than in controls. Within the RA patients’ group the IL-6 level was significantly increased in B19+p patients with disease activity scores of DAS28>5.2, high C-reactive protein and low haemoglobin. Contrary to the healthy controls, the majority of RA B19+p patients did not have antibodies to VP-1S (VP1u) and VP-N (N-terminal half of structural proteins VP1 and VP2), which correspond to the epitopes of neutralizing antibodies. These results indicate that B19V infection at least in some patients is involved in RA pathogenesis.

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Increased expression and secretion of interleukin-6 in human parvovirus B19 non-structural protein (NS1) transfected COS-7 epithelial cells

Abstract: SummaryHuman parvovirus B19 (B19) has been associated with a variety of autoimmune diseases, including rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). We have demonstrated previously that B19 non-structural protein (

Cited by 39 publications

References 28 publications

Human parvovirus B19 and autoimmune diseases. Review of the literature and pathophysiological hypotheses

Human parvovirus B19 and autoimmune diseases. Review of the literature and pathophysiological hypotheses

Green Fluorescent Protein (GFP) Color Reporter Gene Visualizes Parvovirus B19 Non-Structural Segment 1 (NS1) Transfected Endothelial Modification

Frequency and significance of parvovirus B19 infection in patients with rheumatoid arthritis

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