Increased excretion of 5-hydroxy-indole-acetic acid after the administration of 3-indole-acetic acid (heteroauxine)

Rysánek, K; Vítek, V.

doi:10.1007/bf02158112

Cited by 9 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Myotonia and hypothermia were also seen in male albino 23 and male ddY mice 24 after a single administration of 300 mg kg -1 IAA. IAA has reportedly been given to patients and volunteers, with no major toxicities reported after administration of 3-10 g. 25,26 However, no detailed patient or clinical data from these old trials are available.…”

Section: Discussionmentioning

confidence: 99%

In vivo characterization of horseradish peroxidase with indole-3-acetic acid and 5-bromoindole-3-acetic acid for gene therapy of cancer

et al. 2010

View full text Add to dashboard Cite

Gene-directed enzyme prodrug therapy is a form of targeted cancer therapy, in which an enzyme is used to convert a non-toxic prodrug to a cytotoxin within the tumor. Horseradish peroxidase (HRP) is able to convert the indole prodrugs indole-3-acetic acid (IAA) and the halogenated derivative 5-bromo-IAA (5Br-IAA) to toxic agents able to induce cell kill in vitro. This study characterized HRP-directed gene therapy in vivo. Human nasopharyngeal squamous cell carcinoma cells, FaDu, stably expressing HRP were grown as xenografts in SCID mice. Pharmacokinetic analysis of IAA and 5Br-IAA showed satisfactory drug profiles, and millimolar concentrations could be achieved in tumor tissue at non-toxic doses. HRP-expressing tumors showed a modest growth delay when treated with IAA compared with drug-vehicle controls. Treatment response could not be improved using different drug scheduling or drug vehicle, nor by combining HRP-directed gene therapy with fractionated radiotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo characterization of horseradish peroxidase with indole-3-acetic acid and 5-bromoindole-3-acetic acid for gene therapy of cancer

et al. 2010

View full text Add to dashboard Cite

show abstract

“…20) It has also been reported that doses of IAA (40-150 mg/kg) could be safely administered in humans. 21) In another animal study, IAA (50 mg/kg i.p.) was administered to mice and was found to be sufficient to reach the tumor concentrations of IAA three times those used in the in vitro experiments.…”

Section: Discussionmentioning

confidence: 98%

Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination

Park

Kim

2009

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

“… 56 , 57 On the other hand, indole-3-acetic acid is a naturally occurring plant growth phytohormone 58 that can be used as a non-toxic prodrug as it is well-tolerated by humans. 59 , 60 Horseradish peroxidase catalyzes the oxidation of IAA to release free radicals (ie: indolyl, skatolyl, peroxyl radicals) and reactive oxygen species (ROS) (ie, O ·− 2 , and H 2 O 2 ), which induce oxidative stress and cell death by activating apoptotic pathways. 61 , 62 Based on the above, we report herein the preparation of AuNPs decorated with HRP (HRP-AuNCs) and its biological evaluation for EPT applications in combination with the prodrug indole-3-acetic acid (IAA).…”

Section: Introductionmentioning

confidence: 99%

Horseradish Peroxidase-Functionalized Gold Nanoconjugates for Breast Cancer Treatment Based on Enzyme Prodrug Therapy

Vivo-Llorca¹,

Morellá-Aucejo²,

García‐Fernández³

et al. 2022

IJN

View full text Add to dashboard Cite

Introduction Breast cancer has the highest mortality rate among cancers in women. Patients suffering from certain breast cancers, such as triple-negative breast cancer (TNBC), lack effective treatments. This represents a clinical concern due to the associated poor prognosis and high mortality. As an approach to succeed over conventional therapy limitations, we present herein the design and evaluation of a novel nanodevice based on enzyme-functionalized gold nanoparticles to efficiently perform enzyme prodrug therapy (EPT) in breast cancer cells. Results In particular, the enzyme horseradish peroxidase (HRP) – which oxidizes the prodrug indole-3-acetic acid (IAA) to release toxic oxidative species – is incorporated on gold nanoconjugates (HRP-AuNCs), obtaining an efficient nanoplatform for EPT. The nanodevice is biocompatible and effectively internalized by breast cancer cell lines. Remarkably, co-treatment with HRP-AuNCs and IAA (HRP-AuNCs/IAA) reduces the viability of breast cancer cells below 5%. Interestingly, 3D tumor models (multicellular tumor spheroid-like cultures) co-treated with HRP-AuNCs/IAA exhibit a 74% reduction of cell viability, whereas the free formulated components (HRP, IAA) have no effect. Conclusion Altogether, our results demonstrate that the designed HRP-AuNCs nanoformulation shows a remarkable therapeutic performance. These findings might help to bypass the clinical limitations of current tumor enzyme therapies and advance towards the use of nanoformulations for EPT in breast cancer.

show abstract

Increased excretion of 5-hydroxy-indole-acetic acid after the administration of 3-indole-acetic acid (heteroauxine)

Cited by 9 publications

References 9 publications

In vivo characterization of horseradish peroxidase with indole-3-acetic acid and 5-bromoindole-3-acetic acid for gene therapy of cancer

In vivo characterization of horseradish peroxidase with indole-3-acetic acid and 5-bromoindole-3-acetic acid for gene therapy of cancer

Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination

Horseradish Peroxidase-Functionalized Gold Nanoconjugates for Breast Cancer Treatment Based on Enzyme Prodrug Therapy

Contact Info

Product

Resources

About