Increased erythrocyte rigidity in chronic alcoholics without cirrhosis: Deformability improvement of erythrocyte sub-populations after alcohol withdrawal

Guillet, R.; Nalpas, Bertrand; Perrotin, P.; Beuzard, Yves; Koutsouris, D.; Boynard, M.

doi:10.3233/ch-1991-111-208

Cited by 4 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On this basis and supported by the present results, we may underline the adjuvant role of natural antioxidants assumed with food, such as curcumin, in protecting Band 3 function from external stressors, such as increase in ROS levels, which correlate with high glucose blood levels, aging, physical exercise, use of drugs and their metabolites, neurodegenerative diseases and chronic alcohol consumption [ 32 , 37 , 41 , 44 , 45 , 46 ]. In this latter regard, increased blood viscosity, along with impaired erythrocyte flexibility and increased fragility, have been reported as a consequence of alcohol-induced oxidation [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

H2O2-Induced Oxidative Stress Affects SO4= Transport in Human Erythrocytes

Morabito

Romano²,

Spada

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

The aim of the present investigation was to verify the effect of H2O2-induced oxidative stress on SO4= uptake through Band 3 protein, responsible for Cl-/HCO3- as well as for cell membrane deformability, due to its cross link with cytoskeletal proteins. The role of cytoplasmic proteins binding to Band 3 protein has been also considered by assaying H2O2 effects on hemoglobin-free resealed ghosts of erythrocytes. Oxidative conditions were induced by 30 min exposure of human erythrocytes to different H2O2 concentrations (10 to 300 μM), with or without GSH (glutathione, 2 mM) or curcumin (10 μM), compounds with proved antioxidant properties. Since SO4= influx through Band 3 protein is slower and better controllable than Cl- or HCO3- exchange, the rate constant for SO4= uptake was measured to prove anion transport efficiency, while MDA (malondialdehyde) levels and –SH groups were estimated to quantify the effect of oxidative stress. H2O2 induced a significant decrease in rate constant for SO4= uptake at both 100 and 300 μM H2O2. This reduction, observed in erythrocytes but not in resealed ghosts and associated to increase in neither MDA levels nor in –SH groups, was impaired by both curcumin and GSH, whereas only curcumin effectively restored H2O2-induced changes in erythrocytes shape. Our results show that: i) 30 min exposure to 300 μM H2O2 reduced SO4= uptake in human erythrocytes; ii) oxidative damage was revealed by the reduction in rate constant for SO4= uptake, but not by MDA or –SH groups levels; iii) the damage was produced via cytoplasmic components which cross link with Band 3 protein; iv) the natural antioxidant curcumin may be useful in protecting erythrocytes from oxidative injury; v) SO4= uptake through Band 3 protein may be reasonably suggested as a tool to monitor erythrocytes function under oxidative conditions possibly deriving from alcohol consumption, use of drugs, radiographic contrast media administration, hyperglicemia or neurodegenerative diseases.

show abstract