Increased endogenous level of melatonin in preovulatory human follicles does not directly influence progesterone production

Nakamura, Yasuhiko; Tamura, Hiroshi; Takayama, Hisako; Katô, Hiroshi

doi:10.1016/s0015-0282(03)01008-2

Cited by 139 publications

(121 citation statements)

References 29 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…Similarly, this effect has been described in many other species, including rats and sheep (Durotoye et al 1997, Adriaens et al 2006. On the contrary, some reports showed no effects or negative effects of melatonin on P 4 production in the growing and luteinised granulosa cells (Sirotkin 1994, Murayama et al 1997, Schaeffer & Sirotkin 1997, Bodis et al 2001, Nakamura et al 2003. The interpretation of studies on the in vitro effects of melatonin on steroidogenesis is complicated and seems to depend on cell types (theca or granulosa cells), duration of treatment (acute or long-term response), experimental model (cell culture or follicle culture), species, dose and different culture media (Tamura et al 2009).…”

Section: Discussionsupporting

confidence: 61%

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca¹,

Manunta²,

Spezzigu³

et al. 2014

Reproduction

View full text Add to dashboard Cite

This study assessed the effect of melatonin deprival on ovarian status and function in sheep. Experimental procedures were carried out within two consecutive breeding seasons. Animals were divided into two groups: pinealectomised (nZ6) and sham-operated (nZ6). The completeness of the pineal gland removal was confirmed by the plasma concentration of melatonin. Ovarian status was monitored by ovarian ultrasonography for 1 year to study reproductive seasonality. Follicular and corpus luteal growth dynamics were assessed during an induced oestrous cycle. As the effects of melatonin on the ovary may also be mediated by its antioxidant properties, plasma Trolox equivalent antioxidant capacity (TEAC) was determined monthly for 1 year. Pinealectomy significantly extended the breeding season (310G24.7 vs 217.5G24.7 days in controls; P!0.05). Both pinealectomised and sham-operated ewes showed a well-defined wave-like pattern of follicle dynamics; however, melatonin deficiency caused fewer waves during the oestrous cycle (4.3G0.2 vs 5.2G0.2; P!0.05), because waves were 1 day longer when compared with the controls (7.2G0.3 vs 6.1G0.3; P!0.05). The mean area of the corpora lutea (105.4G5.9 vs 65.4G5.9 mm 2 ; P!0.05) and plasma progesterone levels (7.1G0.7 vs 4.9G0.6 ng/ml; P!0.05) were significantly higher in sham-operated ewes compared with pinealectomised ewes. In addition, TEAC values were significantly lower in pinealectomised ewes compared with control ones. These data suggest that melatonin, besides exerting its well-known role in the synchronisation of seasonal reproductive fluctuations, influences the growth pattern of the follicles and the steroidogenic capacity of the corpus luteum. Free Italian abstractAn Italian translation of this abstract is freely available at

show abstract

Section: Discussionsupporting

confidence: 61%

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca¹,

Manunta²,

Spezzigu³

et al. 2014

Reproduction

View full text Add to dashboard Cite

show abstract

“…20 Adriaens et al 21 found that variations in melatonin levels could influence P 4 concentrations. Testing the effects of a range of melatonin doses on in vitro cultured mouse ovarian follicles, they found increased P 4 levels after administration of a low melatonin dose (100 lM); E 2 levels were unaffected.…”

Section: Melatonin Quantity Measuresmentioning

confidence: 99%

The Relationship of Nocturnal Melatonin to Estradiol and Progesterone in Depressed and Healthy Pregnant Women

Posadas

Meliska

Martı́nez

et al. 2012

Journal of Women's Health

View full text Add to dashboard Cite

Purpose: To assess the relationship between nocturnal plasma melatonin and serum estradiol (E 2 ) and progesterone (P 4 ) levels in depressed pregnant women (DW) and matched healthy women (HW). Methods: We used analysis of variance (ANOVA) and linear regression analyses on data obtained from pregnant HW and DW. Results: Log E 2 and log P 4 were positively correlated with measures of melatonin quantity in HW ( p < 0.05) but not DW, controlling for age. Log E 2 and log P 4 were positively correlated with melatonin offset and duration in DW ( p < 0.01) but not HW. Conclusions: Pregnant DW may be less sensitive than HW to modulation of melatonin secretion by E 2 and P 4 . That melatonin timing measures are more sensitive to E 2 and P 4 variation in DW may reflect a circadian system more attuned to the need for realignment in DW than in HW. These altered sensitivities to reproductive hormones may reflect a biologic vulnerability that predisposes some pregnant women to depression.

show abstract

“…This method varies slightly from lab to lab but in general it involves an initial 300-500 × g centrifugation for up to 15-20 min to concentrate the cells. The resuspended cell pellet is then gently layered over a 40-50% Percoll cushion (4)(5)(6) or similarly effective concentrations of Ficoll (7)(8)(9) or in some laboratories, a two-step 40 and 60% discontinuous Percoll gradient is used (10). This is followed by centrifugation at generally around 500 × g for 20 min.…”

Section: Introductionmentioning

confidence: 99%

Ontario, Canada

Lobb

Younglai²

2006

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

Increased endogenous level of melatonin in preovulatory human follicles does not directly influence progesterone production

Cited by 139 publications

References 29 publications

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

The Relationship of Nocturnal Melatonin to Estradiol and Progesterone in Depressed and Healthy Pregnant Women

Ontario, Canada

Contact Info

Product

Resources

About