Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

Abstract

In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2-2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hAD-MSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.

Three-Dimensional Printing of Abrasive, Hard, and Thermally Conductive Synthetic Microdiamond–Polymer Composite Using Low-Cost Fused Deposition Modeling Printer

“…As mentioned above, the microdiamond interlocks the polymer matrix and restricts its mobility, resulting in higher elastic modulus. This significant increase in the elastic modulus is also in agreement with similar previous studies. , The reported elastic moduli of various 3D printable polymers and composites are also given within Table .…”

“…These include its hydrophilicity, biocompatibility, nontoxicity, thermal stability, thermal conductivity, electrical insulation (or indeed electrical conductivity in boron-doped variants), hardness, wear resistance, and low cost. Hence, several studies have already reported the advantages of nano- and microdiamonds as reinforcing enhancers in polymer composites. − …”