Increased efficacy of combining prebiotic and postbiotic in mouse models relevant to autism and depression

Leo, Antonio; De, Carmen; Mainardi, P.; Tallarico, Martina; Nesci, Valentina; Marascio, Nadia; Striano, Pasquale; Russo, Emilio; Constanti, Andrew; Sarro, Giovambattista De; Citraro, Rita

doi:10.1016/j.neuropharm.2021.108782

Cited by 39 publications

(30 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have indicated that the microbiota might play a significant role in major depressive disorders through the microbiome-gut-brain axis ( 42 , 43 ). In addition, intestinal microbiota alterations induced by providing probiotics and prebiotics could effectively improve the depression-like behaviors ( 44 , 45 ). In the present study, we found that microbiota abundance and diversity were unchanged after 6 weeks of CUMS treatment, consistent with previous studies ( 46 , 47 ).…”

Section: Discussionmentioning

confidence: 99%

Combined probiotics attenuate chronic unpredictable mild stress-induced depressive-like and anxiety-like behaviors in rats

Huang

Ze³

et al. 2022

Front. Psychiatry

View full text Add to dashboard Cite

Increasing evidence indicated that probiotics can be effective in improving behaviors similar to depression and anxiety disorders. However, the underlying mechanisms remain unclear, as is the effects of single vs. combined probiotics on depression and anxiety. This study aimed to determine whether combined probiotics could attenuate depressive-like and anxiety-like behavior induced by chronic unpredictable mild stress (CUMS) and its potential mechanisms. Rats underwent CUMS treatment and then administered Lactobacillus rhamnosus HN001 (HN001) or Bifidobacterium animalis subsp. lactis HN019 (HN019), alone or in combination. Levels of neurotransmitters, inflammatory factors, and the gut microbiota were measured. HN001 and (or) HN019 treatment improved depressive-like and anxiety-like behavior in rats, including increased moving distance and exploratory behavior (p < 0.05). In addition, altered gut microbiota structure induced by CUMS was amended by HN001 and/or HN019 (p < 0.05). HN001 and/or HN019 intervention also remarkably normalized levels of 5-HT, DA, NE, HVA, DOPAC, HIAA, TNF-α, IL-6, IL-18 and IL-1β in CUMS rats (p < 0.05). Furthermore, the effects of combined probiotics on decreasing inflammation and improved gut microbiota (Chao1 index and ACE index, p < 0.05) were superior to the single probiotics. Moreover, spearman analysis showed a certain correlation between the different microbiota, such as Firmicutes, Bacteroidetes, Verrucomicrobias, Proteobacterias and Actinobacterias, and inflammation and neurotransmitters. These findings suggested that CUMS induced depressive and anxiety-like behaviors can be alleviated by the combination of probiotics, which was possibly associated with the alterations in the gut microbiota composition and increased neurotransmitters and decreased inflammatory factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined probiotics attenuate chronic unpredictable mild stress-induced depressive-like and anxiety-like behaviors in rats

Huang

Ze³

et al. 2022

Front. Psychiatry

View full text Add to dashboard Cite

show abstract

“…44 The method is well described in our earlier papers 42 and has also been employed to screen the antidepressant-like effects of prebiotics and probiotics. 45 On day 1 of the test, rats were immersed in a cylindrical tank of dimensions 50 cm in height and 25 cm in diameter containing around 30 cm of water with a temperature of 25 °C and allowed to swim for 15 min. After 24 h, individual rats were re-subjected to the same swimming cylinder for a test period of 5 min, and the immobility time was recorded manually by an observer who was blind to the treatment condition.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The increased passivity (immobility) in the swimming tank indicates behavioral despair . The method is well described in our earlier papers and has also been employed to screen the antidepressant-like effects of prebiotics and probiotics . On day 1 of the test, rats were immersed in a cylindrical tank of dimensions 50 cm in height and 25 cm in diameter containing around 30 cm of water with a temperature of 25 °C and allowed to swim for 15 min.…”

Section: Materials and Methodsmentioning

confidence: 99%

Multi-strain Probiotic Formulation Reverses Maternal Separation and Chronic Unpredictable Mild Stress-Generated Anxiety- and Depression-like Phenotypes by Modulating Gut Microbiome–Brain Activity in Rats

Dandekar

Palepu

Satti

et al. 2022

ACS Chem. Neurosci.

View full text Add to dashboard Cite

Depression is a debilitating mental disorder that affects >322 million people worldwide. Despite the availability of several antidepressant agents, many patients remain treatment refractory. A growing literature study has indicated the role of gut microbiota in neuropsychiatric disorders. Herein, we examined the psychobiotic-like activity of multi-strain probiotic formulation in maternal separation (MS) and chronic unpredictable mild stress (CUMS) models of anxiety-and depression-like phenotypes in Sprague-Dawley rats. Early-and late-life stress was employed in both male and female rats by exposing them to MS and CUMS. The multi-strain probiotic formulation (Cognisol) containing Bacillus coagulans Unique IS-2, Lactobacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58, Bifidobacterium lactis UBBLa-70, Bifidobacterium breve UBBr-01, and Bifidobacterium infantis UBBI-01 at a total strength of 10 billion cfu along with L-glutamine was administered for 6 weeks via drinking water. Neurobehavioral assessment was done using the forced swim test (FST), sucrose preference test (SPT), elevated plus maze (EPM), and open field test (OFT). Animals were sacrificed after behavioral assessment, and blood, brain, and intestine samples were collected to analyze the levels of cytokines, metabolites, and neurotransmitters and histology. Animals exposed to stress showed increased passivity, consumed less sucrose solution, and minimally explored the open arms in the FST, SPT, and EPM, respectively. Administration of multi-strain probiotics along with L-glutamine for 6 weeks ameliorated the behavioral abnormalities. The locomotor activity of animals in the OFT and their body weight remained unchanged across the groups. Cognisol treatment reversed the decreased BDNF and serotonin levels and increased CRP, TNF-α, and dopamine levels in the hippocampus and/or frontal cortex. Administration of Cognisol also restored the plasma levels of L-tryptophan, L-kynurenine, kynurenic-acid, and 3hydroxyanthranilic acid; the Firmicutes-to-Bacteroides ratio; the levels of acetate, propionate, and butyrate in fecal samples; the villi/ crypt ratio; and the goblet cell count, which manifested in the restoration of intestinal functions. We suggest that the multi-strain probiotic and glutamine formulation (Cognisol) ameliorated the MS + UCMS-generated anxiety-and depression-like phenotypes by reshaping the gut microbiome−brain activity in both sexes.

show abstract

“…The symbiotic microorganisms within Tibetan kefir grains (TKGs) may offer robust gastrointestinal (GI) immunity due to enriched Lactobacillus kefiranofaciens and Lactobacillus helveticus [ 42 ]. Another reliable approach to reconstructing GI flora represents the synergic effect with psychobiotics ADR-159 [ 43 ] or with pre- (α-lactalbumin) and postbiotics (sodium butyrate) [ 44 ]. There are two registered clinical trials (NCT04741971, NCT04472065) underway.…”

Section: Resultsmentioning

confidence: 99%

An Updated Narrative Mini-Review on the Microbiota Changes in Antenatal and Post-Partum Depression

Doroftei

Ilie

Diaconu³

et al. 2022

Diagnostics

View full text Add to dashboard Cite

Background: Antenatal depression (AND) and post-partum depression (PPD) are long-term debilitating psychiatric disorders that significantly influence the composition of the gut flora of mothers and infants that starts from the intrauterine life. Not only does bacterial ratio shift impact the immune system, but it also increases the risk of potentially life-threatening disorders. Material and Methods: Therefore, we conducted a narrative mini-review aiming to gather all evidence published between 2018–2022 regarding microflora changes in all three stages of pregnancy. Results: We initially identified 47 potentially eligible studies, from which only 7 strictly report translocations; 3 were conducted on rodent models and 4 on human patients. The remaining studies were divided based on their topic, precisely focused on how probiotics, breastfeeding, diet, antidepressants, exogenous stressors, and plant-derived compounds modulate in a bidirectional way upon behavior and microbiota. Almost imperatively, dysbacteriosis cause cognitive impairments, reflected by abnormal temperament and personality traits that last up until 2 years old. Thankfully, a distinct technique that involves fecal matter transfer between individuals has been perfected over the years and was successfully translated into clinical practice. It proved to be a reliable approach in diminishing functional non- and gastrointestinal deficiencies, but a clear link between depressive women’s gastrointestinal/vaginal microbiota and clinical outcomes following reproductive procedures is yet to be established. Another gut-dysbiosis-driving factor is antibiotics, known for their potential to trigger inflammation. Fortunately, the studies conducted on mice that lack microbiota offer, without a shadow of a doubt, insight. Conclusions: It can be concluded that the microbiota is a powerful organ, and its optimum functionality is crucial, likely being the missing puzzle piece in the etiopathogenesis of psychiatric disorders.

show abstract

Increased efficacy of combining prebiotic and postbiotic in mouse models relevant to autism and depression

Cited by 39 publications

References 55 publications

Combined probiotics attenuate chronic unpredictable mild stress-induced depressive-like and anxiety-like behaviors in rats

Combined probiotics attenuate chronic unpredictable mild stress-induced depressive-like and anxiety-like behaviors in rats

Multi-strain Probiotic Formulation Reverses Maternal Separation and Chronic Unpredictable Mild Stress-Generated Anxiety- and Depression-like Phenotypes by Modulating Gut Microbiome–Brain Activity in Rats

An Updated Narrative Mini-Review on the Microbiota Changes in Antenatal and Post-Partum Depression

Contact Info

Product

Resources

About