Increased Dynamin‐Related Protein 1–Dependent Mitochondrial Fission Contributes to High‐Fat‐Diet‐Induced Cardiac Dysfunction and Insulin Resistance by Elevating Tafazzin in Mouse Hearts

Chang, Wenguang; Xiao, Dan; Ao, Xiang; Li, Mengyang; Xu, Tao; Jx, Wang

doi:10.1002/mnfr.201801322

Cited by 12 publications

(5 citation statements)

References 49 publications

(62 reference statements)

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“…In the present study, four control diets, namely KX, HF, LD, and RD diets, were selected as they are commonly used in animal experiments. Consistent with the reported works of literature, 31‐36 throughout the animal feeding period, no instances of mortality or clinical signs indicating adverse effects related to the diets were observed in the mice. There were no significant discrepancies in the physiological parameter among the groups of mice, including body weight, visceral weight, and blood biochemical indices.…”

Section: Discussionsupporting

confidence: 91%

Differential effects of four laboratory animal control diets on gut microbiota in mice

Guo,

Zhao,

Kang

et al. 2024

J Sci Food Agric

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BACKGROUNDThe gut microbiota is intricate and susceptible to multiple factors, with diet being a major contributor. The present study aimed to investigate the impact of four commonly used laboratory animal control diets, namely Keao Xieli's maintenance diet (KX), HFK's 1025 (HF), Research Diets' D12450B (RD), and Lab Diet's 5CC4 (LD), on the gut microbiota of mice.RESULTSA total of 40 mice were randomly assigned to four groups, and each group was fed one of the four diets for a duration of 8 weeks. The assessment of gut microbiota was conducted using 16S rRNA sequencing both at the beginning of the study (week 0) and the end (week 8), which served as the baseline and endpoint samples, respectively. Following the 8‐week feeding period, no significant differences were observed in physiological parameters, including body weight, visceral weight, and blood biochemical indices, across the four groups. Nonetheless, relative to the baseline, discernible alterations in the gut microbiota were observed in all groups, encompassing shifts in beta‐diversity, hierarchical clustering, and key genera. Among the four diets, HF diet exhibited a significant influence on alpha‐diversity, RD diet brought about notable changes in microbial composition at the phylum level, and LD diet demonstrated an interconnected co‐occurrence network. Mantel analysis indicated no significant correlation between physiological parameters and gut microbiota in the four groups.CONCLUSIONOverall, our study demonstrated that the four control diets had a minimal impact on physiological parameters, while exerting a distinct influence on the gut microbiota after 8 weeks. © 2024 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 91%

Differential effects of four laboratory animal control diets on gut microbiota in mice

Guo,

Zhao,

Kang

et al. 2024

J Sci Food Agric

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“…The overload of nutrients impairs cardiac mitochondrial function, leading to decreased mitochondrial biogenesis, reduced mitochondrial coupling efficiency and decreased ATP synthesis . Mice fed a HFD have increased DRP1‐dependent mitochondrial fission which is linked to increased oxidative stress, decreased ATP production, insulin resistance and cardiac dysfunction . Rats fed a HFD have cardiometabolic dysfunction, myocardial mitochondrial dysfunction and impairment of mitochondrial dynamics; the HFD not only downregulates mitochondrial fusion‐related proteins but also upregulates fission‐related proteins …”

Section: Introductionmentioning

confidence: 99%

“…14,15 Mice fed a HFD have increased DRP1-dependent mitochondrial fission which is linked to increased oxidative stress, decreased ATP production, insulin resistance and cardiac dysfunction. 16,17 Rats fed a HFD have cardiometabolic dysfunction, myocardial mitochondrial dysfunction and impairment of mitochondrial dynamics; the HFD not only downregulates mitochondrial fusion-related proteins but also upregulates fission-related proteins. 11,12 In our previous study, we established a myocardial fibrosis minipig model via HFD feeding.…”

Section: Introductionmentioning

confidence: 99%

The impact of DRP1 on myocardial fibrosis in the obese minipig

Chen

Wang

et al. 2020

Eur J Clin Investigation

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Background The heart is a highly oxidative tissue, thus mitochondria play a major role in maintaining optimal cardiac function. Our previous study established a dietary‐induced obese minipig with cardiac fibrosis. The aim of this study was to elucidate the role of mitochondrial dynamics in cardiac fibrosis of obese minipigs. Design Four‐month‐old Lee‐Sung minipigs were randomly divided into two groups: a control group (C) and an obese group (O) by feeding a control diet or a high‐fat diet (HFD) for 6 months. Exposure of H9c2 cardiomyoblasts to palmitate was used to explore the effects of high‐fat on induction of myocardial injury in vitro. Results The O pigs displayed greater heart weight and cardiac collagen accumulation. Obese pigs exhibited a lower antioxidant capacity, ATP concentration, and higher oxidative stress in the left ventricle (LV). The HFD caused downregulation in protein expression of PGC‐1α and OPA1, and upregulation of DRP1, FIS1, and PINK1 in the LV of O compared to C pigs. Furthermore, palmitate induced apoptosis and decreased ATP content in H9c2 cells. Palmitate elevated the protein expression of DRP1 and PINK1 in these cells. Inhibition of DRP1 protein expression by siDRP1 in H9c2 cells resulted in enhanced ATP and decreased palmitate‐induced apoptosis. Conclusions These results suggest that mitochondrial dynamics were linked to the progression of obesity‐related cardiac injury. Inhibition of DRP1 after palmitate exposure in H9c2 cells resulted in improved ATP level and decreased apoptosis in vitro suggesting that mitochondrial fission serves a key role in progression of obesity‐induced cardiac fibrosis.

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“…if the rat weighs 150 gm, curcumin dose for this rat will be 37.5 mg suspended in 1 ml saline given by oral gavage). Before scarification, transthoracic 2D mode echo-cardiogram was done to measure: left ventricular posterior wall thickness at end-diastole (LVPWd), left ventricular dimensions at end-systole (LVDs), left ventricular posterior wall thickness at endsystole (LVPWs), ejection fraction (EF %) and fractional shortening (FS %) [16] .…”

Section: Methodsmentioning

confidence: 99%

The Effect of Dipeptidyl Peptidase-4 Inhibitors or Curcumin on Diet Induced Metabolic Syndrome with Cardiac Dysfunction in Rats

Etewa

El-Arab

Rahman

et al. 2020

Journal of Recent Advances in Medicine

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Background: Metabolic syndrome (MS) increases the risk of developing cardiovascular diseases (CVDs) which are the leading cause of death worldwide.Objective: This study is aiming to investigate the curative effects of dipeptidyl peptidase-4 inhibitors and the prophylactic effects of curcumin on diet induced metabolic syndrome which is associated with cardiac dysfunction in rats.Methodology: sixty adult male albino rats were divided into six groups: Group I: (control). Group II: (MS induced). Group III: (Vildagliptin only). Group IV: (Curcumin only). Group V (therapeutic): Vildagliptin treated rats after 8 weeks of MS to the end of 12 weeks. Group VI (prophylactic): Curcumin is administered with MS induction concomitantly for 12 weeks. Transthoracic echocardiogram was done while rats were alive. At the end of the experiment, blood was collected and biochemical analysis (blood glucose, serum insulin, lipid profile and cardiac enzymes) was done. Heart tissues were used for oxidative stress parameters (cardiac mitochondrial reactive oxygen species (ROS) and cardiac mitochondrial complex I and complex II) beside histopathological examination.Results: High fat diet (HFD) administration resulted in a significant increase in blood glucose, serum insulin, homeostasis model assessment of IR (HOMA-IR) index with disturbed lipid profile. Significant increase in serum cardiac enzymes and oxidative stress tissue markers were also noticed. Echocardiography revealed structural and functional cardiomyopathy. Histopathological examination showed cellular infiltrations with fat cells and collagen fibers accumulation. DPP-4 inhibitors and curcumin resulted in a significant decrease in glucose, insulin, and HOMA-IR index, partial amelioration of lipid profile, cardiac enzymes, and cardiac oxidative stress markers. Echocardiography revealed alleviation of cardiomyopathy. Histopathological examination also revealed highly manifested structural improvement. Conclusion:The current study revealed that both DPP-4 inhibitors and curcumin improve cardiomyopathy resulted from HFD administration with a diversity of mechanisms including anti-inflammatory, anti-apoptotic and anti-oxidative ways.

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Increased Dynamin‐Related Protein 1–Dependent Mitochondrial Fission Contributes to High‐Fat‐Diet‐Induced Cardiac Dysfunction and Insulin Resistance by Elevating Tafazzin in Mouse Hearts

Cited by 12 publications

References 49 publications

Differential effects of four laboratory animal control diets on gut microbiota in mice

Differential effects of four laboratory animal control diets on gut microbiota in mice

The impact of DRP1 on myocardial fibrosis in the obese minipig

The Effect of Dipeptidyl Peptidase-4 Inhibitors or Curcumin on Diet Induced Metabolic Syndrome with Cardiac Dysfunction in Rats

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