Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry

Dierikx, Cindy; Essen-Zandbergen, Alieda van; Veldman, Kees; Smith, Hilde E.; Mevius, Dik

doi:10.1016/j.vetmic.2010.03.019

Cited by 191 publications

(161 citation statements)

References 20 publications

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“…From week 12 onwards, in at least one isolate of every sampling moment, ESBL/pAmpC genes were typed by PCR and sequencing (Dierikx et al, 2010). Plasmids were characterized by transformation (Dierikx et al, 2010) and PCR-based Replicon Typing (PBRT) (Diatheva, Italy).…”

Section: Typingmentioning

confidence: 99%

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Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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A B S T R A C TExtended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pAmpC) producing bacteria are resistant to Extended Spectrum Cephalosporins (ESC), and are present in all levels of the broiler production chain. We determined the prevalence, concentration, and persistence of ESBL/pAmpC-Escherichia coli in a broiler parent flock during the rearing and laying period. One-day old chickens were housed in four separate pens. Until week 33 no antibiotics or coccidiostatics were used. During rearing 57 chickens in each pen (n = 228), and in the laying period two groups of 33 chickens were individually sampled (n = 66). Environmental samples were taken from week 16 onwards. ESBL/pAmpC-E. coli presence was determined by selective culturing. In the samples of week 16-19 the concentration of ESBL/pAmpC-E. coli was determined. All ESC-resistant isolates found were positive for pAmpC gene bla CMY-2 located on IncA/C plasmids, in several E. coli MLST types. CMY-2-E. coli prevalence decreased from 91% (95%CI 86-94%) at day 7 (week 1) to 0% (95%CI 0-5%) in week 21. However, CMY-2-E. coli remained present in the environmental samples during the whole study. CMY-2-E. coli concentration varied between detection limit (< 10^3) and 2·10^4 cfu/g faeces. The sharp reduction of CMY-2-E. coli in this broiler parent flock in absence of antibiotics suggests a selective disadvantage of bla CMY-2 on IncA/C plasmids on animal level. The underlying mechanism should be studied further as this may provide new insights on how to reduce ESBL/pAmpC prevalence and transmission in the broiler production chain.

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Section: Typingmentioning

confidence: 99%

“…Plasmids were characterized by transformation (Dierikx et al, 2010) and PCR-based Replicon Typing (PBRT) (Diatheva, Italy). Selection of transformants was performed on LB agar …”

Section: Typingmentioning

confidence: 99%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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“…The emergence of MRSA was paralleled by occurrence of ESBL-producing organisms in livestock production. Since 2002 the occurrence of ESBLproducing organisms has been observed in broilers (Dierikx et al 2010). Both in E. coli and in Salmonella a rapid increase was observed of ESBL-producers in these animals.…”

Section: Events Leading To Increased Attention For High Veterinary Usmentioning

confidence: 99%

Reduction of antibiotic use in animals “let’s go Dutch”

Mevius

Heederik

2014

J. Verbr. Lebensm.

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“…Percentages of samples of food-producing animals or food in which ESBL-carrying E. coli isolates were detected varied from 0.2% to 40.1%, according to the country and the methodology used [ (Smet et al, 2009); Table 6]. Occurrences in the range of 10-40% were found when healthy poultry or pigs were analysed for the presence of ESBL-positive E. coli isolates in Portugal (Costa et al, 2009;Goncalves et al, 2010;Machado et al, 2008), Netherlands (Dierikx et al, 2010a), France (Girlich et al, 2007), and slightly lower percentages were identified in Czech Republic (Kolar et al, 2010). Moreover, ESBL-carrying E. coli isolates were found in most of pig and poultry farms tested in Spain , and also in five poultry farms tested in Italy (Bortolaia et al, 2010b).…”

Section: Prevalence Of Esbl-producing E Coli and Salmonella In Food-mentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

236

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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Increased detection of extended spectrum beta-lactamase producing Salmonella enterica and Escherichia coli isolates from poultry

Cited by 191 publications

References 20 publications

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Reduction of antibiotic use in animals “let’s go Dutch”

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

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