Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

Zhang, Xiaoyan; Wei, Junyu; Huang, Yifan; Shen, Wenbiao; Chen, Xin; Lu, Chungui; Su, Nana; Chen, Jin

doi:10.3389/fpls.2018.01020

Cited by 26 publications

(13 citation statements)

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“…IP3 can induce isoflavone accumulation in soybean sprouts by upregulating the activity of isoflavone biosynthetic enzyme under UV-B radiation (Jiao et al, 2016). Similarly, there is a high correlation between IP3 content and anthocyanin accumulation in radish sprouts (Zhang et al, 2018a). In this study, exogenous calcium increased the IP3 content in hypocotyls, and there was a significant correlation with the anthocyanin content (Figure 4).…”

Section: Discussionsupporting

confidence: 61%

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Calcium Positively Mediates Blue Light-Induced Anthocyanin Accumulation in Hypocotyl of Soybean Sprouts

Yue

Song

et al. 2021

Front. Plant Sci.

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Soybean sprouts are a flavorful microgreen that can be eaten all year round and are widely favored in Southeast Asia. In this study, the regulatory mechanism of calcium on anthocyanin biosynthesis in soybean sprouts under blue light was investigated. The results showed that blue light, with a short wavelength, effectively induced anthocyanin accumulation in the hypocotyl of soybean sprout cultivar “Dongnong 690.” Calcium supplementation further enhanced anthocyanin content, which was obviously inhibited by LaCl3 and neomycin treatment. Moreover, exogenous calcium changed the metabolism of anthocyanins, and seven anthocyanin compounds were detected. The trend of calcium fluorescence intensity in hypocotyl cells, as well as that of the inositol 1,4,5-trisphosphate and calmodulin content, was consistent with that of anthocyanins content. Specific spatial distribution patterns of calcium antimonate precipitation were observed in the ultrastructure of hypocotyl cells under different conditions. Furthermore, calcium application upregulated the expression of genes related to anthocyanin biosynthesis, and calcium inhibitors suppressed these genes. Finally, transcriptomics was performed to gain global insights into the molecular regulation mechanism of calcium-associated anthocyanin production. Genes from the flavonoid biosynthesis pathway were distinctly enriched among the differentially expressed genes, and weighted gene co-expression network analysis showed that two MYBs were related to the accumulation of anthocyanins. These results indicated that calcium released from apoplast and intracellular stores in specific spatial-temporal features promote blue light-induced anthocyanin accumulation by upregulation of the expression of genes related to anthocyanin synthesis of “Dongnong 690” hypocotyl. The findings deepen the understanding of the calcium regulation mechanism of blue light-induced anthocyanin accumulation in soybean sprouts, which will help growers produce high-quality foods beneficial for human health.

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Section: Discussionsupporting

confidence: 61%

“…The total anthocyanin content was extracted from the corresponding hypocotyl tissue using an extraction solution (49.9% ddH 2 O, v/v; 50% methanol, v/v; and 0.1% HCl v/v), and measured as described in the previous study (Zhang et al, 2018a).…”

Section: Anthocyanin Extraction and Measurementmentioning

confidence: 99%

Calcium Positively Mediates Blue Light-Induced Anthocyanin Accumulation in Hypocotyl of Soybean Sprouts

Yue

Song

et al. 2021

Front. Plant Sci.

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“…And the up-regulated ZmSOS1 and ZmSKOR in the combined HRW and NaCl treatment might suggested positive role of above 2 genes under saline conditions, which also agreed with the higher K + /Na + ratio in the combined treatment. The SOS pathway is activated by cytosolic Ca 2+ , Zhang demonstrated that HRW treatment could increase the cytosolic Ca 2+ [29]. Here, CDPK21 was of especially interest to us, because it was much more responsive to HRW.…”

Section: Discussionmentioning

confidence: 91%

RETRACTED: Hydrogen-Rich Water Improvement of Root Growth in Maize Exposed to Saline Stress

Yang¹,

Jingyun²,

Zhu³

et al. 2020

Preprint

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Background: Hydrogen gas (H2) is a newly-discovered signaling molecular that plays an important role in plants. This study investigated physiological and molecular mechanisms of hydrogen-rich water (HRW)-mediated beneficial effects on maize roots exposed to saline stress. Results: The results showed that growth of maize seedlings treated with 150 mM NaCl was greatly reduced. Under saline stress, 50% HRW diminished lipid damage in root which was confirmed by malondialdehyde (MDA) content assay and root histochemical staining, and the decreased activities of dismutase (SOD) and peroxidase (POD) further verified the reduced oxidant damage in roots cells under saline stress. HRW up-regulated the expression of ZmSOS1, ZmSKOR, and especially CDPK21 under saline stress, and it also stimulated the activities of PM H+-ATPase and tonoplast H+-ATPase and H+-PPase in maize roots. Thereby, Na+ content was decreased and K+ uptake was increased with the application of HRW. Conclusion: In summary, under saline stress, exogenous HRW application on maize roots up-regulated the key genes expression, improved H+-transport activity and thereby maintained the Na+/K+ balance, diminished oxidant damage and therefore promoted the root growth and biomass accumulation. Our results suggested exogenous HRW treatment on maize could improve root development under saline conditions and might be applied to alleviate salinity stress.

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“…The calcium accumulation in root cells was measured by the Fluo-3/AM (calcium fluorescent probes; catalog no. 39294, Sigma-Aldrich) based on the method of Yan et al (2015) and Zhang et al (2018). Briefly, the root samples were immersed in incubation solution (containing 20 mM Fluo-3/AM, 0.5 M mannitol, 4 mM MES [pH 5.7] and 20 mM KCl) for 30 min at a room temperature.…”

Section: Histochemical Detection Of H 2 O 2 and Ca 21mentioning

confidence: 99%

“…Jin et al (2016) found that under drought stress, H 2 rapidly increased H 2 O 2 and modified the apoplastic pH of leaves in alfalfa via an abscisic acid-based mechanism. Furthermore, incremental IP3dependent cytosolic Ca 21 contributes to H 2 -promoted anthocyanin biosynthesis under UV-A irradiation in radish sprouts (Zhang et al, 2018).…”

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confidence: 93%

Calcium-Dependent Hydrogen Peroxide Mediates Hydrogen-Rich Water-Reduced Cadmium Uptake in Plant Roots

Huang

et al. 2020

Plant Physiol.

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Hydrogen gas (H 2) has a possible signaling role in many developmental and adaptive plant responses, including mitigating the harmful effects of cadmium (Cd) uptake from soil. We used electrophysiological and molecular approaches to understand how H 2 ameliorates Cd toxicity in pak choi (Brassica campestris ssp. chinensis). Exposure of pak choi roots to Cd resulted in a rapid increase in the intracellular H 2 production. Exogenous application of hydrogen-rich water (HRW) resulted in a Cd-tolerant phenotype, with reduced net Cd uptake and accumulation. We showed that this is dependent upon the transport of calcium ions (Ca 21) across the plasma membrane and apoplastic generation of hydrogen peroxide (H 2 O 2) by respiratory burst oxidase homolog (BcRbohD). The reduction in root Cd uptake was associated with the application of exogenous HRW or H 2 O 2. This reduction was abolished in the iron-regulated transporter1 (Atirt1) mutant of Arabidopsis (Arabidopsis thaliana), and pak choi pretreated with HRW showed decreased BcIRT1 transcript levels. Roots exposed to HRW had rapid Ca 21 influx, and Cdinduced Ca 21 leakage was alleviated. Two Ca 21 channel blockers, gadolinium ion (Gd 31) and lanthanum ion (La 31), eliminated the HRW-induced increase in BcRbohD expression, H 2 O 2 production, and Cd 21 influx inhibition. Collectively, our results suggest that the Cd-protective effect of H 2 in plants may be explained by its control of the plasma membrane-based NADPH oxidase encoded by RbohD, which operates upstream of IRT1 and regulates root Cd uptake at both the transcriptional and functional levels. These findings provide a mechanistic explanation for the alleviatory role of H 2 in Cd accumulation and toxicity in plants.

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Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

Cited by 26 publications

References 73 publications

Calcium Positively Mediates Blue Light-Induced Anthocyanin Accumulation in Hypocotyl of Soybean Sprouts

Calcium Positively Mediates Blue Light-Induced Anthocyanin Accumulation in Hypocotyl of Soybean Sprouts

RETRACTED: Hydrogen-Rich Water Improvement of Root Growth in Maize Exposed to Saline Stress

Calcium-Dependent Hydrogen Peroxide Mediates Hydrogen-Rich Water-Reduced Cadmium Uptake in Plant Roots

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