Increased circulating concentrations of interleukin 2 receptor during rejection episodes in heart- or kidney-transplant recipients

Zucchelli, G.C.; Clerico, A.; Maria, Renata De; Carmellini, Mario; Stefano, Rossella Di; Masini, S.; Pilo, A; Donato, L.

doi:10.1093/clinchem/36.12.2106

Cited by 11 publications

(6 citation statements)

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“…An association of plasma sIL-2R with rejection was found in several previous studies (24)(25)(26)(27)(28). Importantly, increased levels of sIL-2R are not specific for rejection because such levels are also found during infection and acute tubular necrosis (ATN) (24)(25)(26)(27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Pre‐transplant Th1 and post‐transplant Th2 cytokine patterns are associated with early acute rejection in renal transplant recipients

Sadeghi¹,

Daniel²,

Weimer³

et al. 2003

Clinical Transplantation

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In this retrospective study, we tried to define pre- and post-transplant immunological parameters that identify patients at risk for early acute rejection. Lymphocyte subpopulations and plasma levels of cytokines and neopterin were determined pre- and post-transplant in 32 renal transplant recipients with biopsy-proven early acute graft rejection. Recipients without early acute rejection served as controls. High pre-transplant interferon-gamma (IFN-gamma) plasma levels (p = 0.006), consistently high levels of neopterin early post-transplant (p = 0.008), a post-transplant switch from a Th1 to a Th2 cytokine pattern with decreasing IFN-gamma (p = 0.02), low CD8+ lymphocyte counts (p = 0.006) and consistently high CD19+ B lymphocyte counts were associated with acute rejection. Our data suggest that patients with a pre-transplant Th1 and an early post-transplant Th2 cytokine pattern are pre-disposed for early acute rejection.

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Section: Discussionmentioning

confidence: 99%

Pre‐transplant Th1 and post‐transplant Th2 cytokine patterns are associated with early acute rejection in renal transplant recipients

Sadeghi¹,

Daniel²,

Weimer³

et al. 2003

Clinical Transplantation

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“…sIL-2R is secreted by activated CD4 + T cells, 11,12 and the sIL-2R concentration has been proposed as a marker of rejection episodes after organ transplantation. 13,14 Acute renal allograft rejection is mediated by T lymphocytes; T cells expressing cell surface IL-2R were present in the kidneys of renal allograft recipients. 15 Prediction of sIL-2R levels might aid in early detection of rejection episodes.…”

Section: Discussionmentioning

confidence: 99%

Human Articular Chondrocytes Induce Interleukin-2 Nonresponsiveness to Allogeneic Lymphocytes

2016

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Introduction. We previously showed that articular chondrocytes (ACs) have immune privilege and immunomodulatory functions like those of mesenchymal stem cells. To elucidate these mechanisms, we focused on interleukin-2 (IL-2), which plays critical roles in lymphocyte mitogenic activity. The purpose of this study was to explore whether ACs affect the role of IL-2 underlying immunomodulatory functions. Material and Methods. Irradiated human ACs from osteoarthritis donors were used. Third-party ACs were added to the mixed lymphocyte reaction (MLR) with or without recombinant human IL-2 (rhIL-2), and the levels of IL-2 and the soluble form of the IL-2 receptor α (sIL-2Rα) protein in supernatant were measured by enzyme-linked immunosorbent assay. Recombinant human IL-2 (rhIL-2) was also added to the MLR. To detect the expression of IL-2 receptor α (CD25) on lymphocytes in the MLR, flow cytometric analysis was performed. Last, ACs and allogeneic activated CD4 + T cell were co-cultured, and the expression of CD25 on activated T cells was examined by flow cytometry. Results. Third-party ACs significantly inhibited the MLR and reduced the level of sIL-2Rα in a dose-dependent manner, but did not affect the concentration of IL-2. Exogenous rhIL-2 accelerated MLR but did not rescue the inhibitory effect of ACs. ACs inhibited the expression of CD25 on activated CD4 + T cells. Discussion. Our results showed that thirdparty ACs inhibited the proliferation of allogeneic activated lymphocytes, thereby inhibiting production sIL-2Rα, although ACs did not affect IL-2 secretion from lymphocytes. Also, ACs inhibited CD25 expression on activated CD4 + T cells. Thus, ACs inhibited the immune response of allogeneic lymphocytes by inducing IL-2 nonresponsiveness.

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“…The sensitivity and specificity to predict rejection were better than chance (71% and 73%, respectively). It has to be considered that sIL‐2R can also increase during inflammatory conditions such as viral or bacterial infections, treatment with anti‐thymocyte globulin, acute tubular necrosis, and cyclosporine toxicity (19–22).…”

Section: Discussionmentioning

confidence: 99%

Serum soluble interleukin 2 receptor (sIL‐2R) as a marker of acute rejection in renal transplant children

García-Roca

Vargas²,

Fuentes

et al. 2012

Pediatric Transplantation

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The aim of the study was to evaluate whether or not serum levels of soluble interleukin 2 receptor (sIL-2R) predict acute rejection in pediatric recipients. We studied 51 pediatric renal transplant recipients divided into three groups: Group 1) Biopsy-proven cellular acute rejection (n = 19), Group 2) Graft dysfunction with histological diagnosis other than acute rejection (n = 8) and Group 3) Patients with stable graft function, no biopsy (n = 24). Serum samples for sIL-2R measurement by sandwich ELISA were obtained at the time of renal transplant and at the time of renal biopsy due to graft dysfunction (Groups 1 and 2) or at six months post-transplant in the case of Group 3. The mean ± s.e. serum values of sIL-2R were higher in patients during acute rejection (6539 ± 1802 pg/mL) compared to patients with other causes of graft dysfunction (2217 ± 256 pg/mL) or stable graft function at six months (2183 ± 283 pg/mL) (Kruskal-Wallis p = 0.004). When the sIL2-R levels at the time of transplant were compared to those at the time of biopsy (Groups 1 and 2) or at six months post-transplant in Group 3, there was no significant difference between baseline and biopsy in the acute rejection group (paired t-test = 0.07), whereas there was a significant reduction in Groups 2 and 3.

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Increased circulating concentrations of interleukin 2 receptor during rejection episodes in heart- or kidney-transplant recipients

Cited by 11 publications

References 0 publications

Pre‐transplant Th1 and post‐transplant Th2 cytokine patterns are associated with early acute rejection in renal transplant recipients

Pre‐transplant Th1 and post‐transplant Th2 cytokine patterns are associated with early acute rejection in renal transplant recipients

Human Articular Chondrocytes Induce Interleukin-2 Nonresponsiveness to Allogeneic Lymphocytes

Serum soluble interleukin 2 receptor (sIL‐2R) as a marker of acute rejection in renal transplant children

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