Increased circulating CD31+/CD42b-EMPs in Perthes disease and inhibit HUVECs angiogenesis via endothelial dysfunction

Li, Boxiang; Huang, Qian; Lin, Chengsen; Lu, Rongbin; Wang, Tiantian; Chen, Xianxiang; Liu, Zheng‐Tang; Liu, Yun; Wu, Jianping; Wu, Yanjiao; Liao, Shijie; Ding, Xiaofei

doi:10.1016/j.lfs.2020.118749

Cited by 15 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human umbilical vascular endothelial cells (HUVECs) were cultured as described previously ( 49 ). HUVECs were seeded into Matrigel™-coated 96-well plates (3×10 4 cells/well) and the cells (MG63, U-2 OS, Saos-2 or 143B cells) were treated either with OSCM or NOSCM for 8 h. Subsequently, the total length and number of junctions were quantified using ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma

et al. 2021

Self Cite

View full text Add to dashboard Cite

Osteosarcoma (OS), which occurs most commonly in adolescents, is associated with a high degree of malignancy and poor prognosis. In order to develop an accurate treatment for OS, a deeper understanding of its complex tumor microenvironment (TME) is required. In the present study, tissues were isolated from six patients with OS, and then subjected to single-cell RNA sequencing (scRNA-seq) using a 10× Genomics platform. Multiplex immunofluorescence staining was subsequently used to validate the subsets identified by scRNA-seq. ScRNA-seq of six patients with OS was performed prior to neoadjuvant chemotherapy, and data were obtained on 29,278 cells. A total of nine major cell types were identified, and the single-cell transcriptional map of OS was subsequently revealed. Identified osteoblastic OS cells were divided into five subsets, and the subsets of those osteoblastic OS cells with significant prognostic correlation were determined using a deconvolution algorithm. Thereby, different transcription patterns in the cellular subtypes of osteoblastic OS cells were reported, and key transcription factors associated with survival prognosis were identified. Furthermore, the regulation of osteolysis by osteoblastic OS cells via receptor activator of nuclear factor kappa-B ligand was revealed. Furthermore, the role of osteoblastic OS cells in regulating angiogenesis through vascular endothelial growth factor-A was revealed. C3_TXNIP+ macrophages and C5_IFIT1+ macrophages were found to regulate regulatory T cells and participate in CD8+ T cell exhaustion, illustrating the possibility of immunotherapy that could target CD8+ T cells and macrophages. Our findings here show that the role of C1_osteoblastic OS cells in OS is to promote osteolysis and angiogenesis, and this is associated with survival prognosis. In addition, T cell depletion is an important feature of OS. More importantly, the present study provided a valuable resource for the in-depth study of the heterogeneity of the OS TME.

show abstract

Section: Methodsmentioning

confidence: 99%

Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because MVs and mitochondrial components are related to endothelial dysfunction[ 18 ] and inflammation[ 19 ], we analysed the relationships among MVs, mitoMVs and cytokines in sepsis. In the sepsis group, the number of mitoMVs was related to expression of TNF-α ( P = 0.022, r = 0.521; Figure 4B , respectively) and to expression of sVCAM-1 ( P = 0.041, r = 0.472; Figure 4C , respectively).…”

Section: Resultsmentioning

confidence: 99%

“…MVs are the medium of information transmission between cells, and many studies have shown that MVs produced under pathological conditions have the ability to induce activation of recipient cells[ 18 , 36 ]. Therefore, based on the above inference, we explored the effect of plasma MVs on ECs in vitro .…”

Section: Discussionmentioning

confidence: 99%

Microvesicles with mitochondrial content are increased in patients with sepsis and associated with inflammatory responses

Zhang¹,

Li²,

Wang³

et al. 2023

World J Clin Cases

View full text Add to dashboard Cite

BACKGROUND Endothelial activation plays an important role in sepsis-mediated inflammation, but the triggering factors have not been fully elucidated. Microvesicles carrying mitochondrial content (mitoMVs) have been implicated in several diseases and shown to induce endothelial activation. AIM To explore whether mitoMVs constitute a subset of MVs isolated from plasma of patients with sepsis and contribute to endothelial activation. METHODS MVs were isolated from human plasma and characterized by confocal microscopy and flow cytometry. Proinflammatory cytokines, including interleukin (IL)-6, IL-8 and tumour necrosis factor (TNF)-α, and soluble vascular cell adhesion molecule (sVCAM)-1 were detected by ELISA. Human umbilical vein endothelial cells (HUVECs) were stimulated with the circulating MVs to evaluate their effect on endothelial activation. RESULTS MitoMVs were observed in plasma from patients with sepsis. Compared with those in healthy controls, expression of MVs, mitoMVs, proinflammatory cytokines and sVCAM-1 was increased. The number of mitoMVs was positively associated with TNF-α and sVCAM-1. In vitro , compared with MVs isolated from the plasma of healthy controls, MVs isolated from the plasma of patients with sepsis induced expression of OAS2, RSAD2 , and CXCL10 in HUVECs. MitoMVs were taken up by HUVECs, and sonication of MVs significantly reduced the uptake of mitoMVs by HUVECs and expression of the above three type I IFN-dependent genes. CONCLUSION MitoMVs are increased in the plasma of patients with sepsis, which induces elevated expression of type I IFN-dependent genes. This suggests that circulating mitoMVs activate the type I IFN signalling pathway in endothelial cells and lead to endothelial activation.

show abstract

“…Scholars also found that necrotic bone stimulates macrophage inflammation through TLR4 in the piglet model of Perthes disease 4 . Our previous studies also showed that plasma in Perthes patients can induce endothelial cell apoptosis and further cause endothelial dysfunction 5 . However, sample collection of hip synovial fluid in clinics is difficult.…”

Section: Introductionmentioning

confidence: 85%

Platelet to lymphocyte ratio was a risk factor in Perthes disease

Wang¹,

Luo

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

The study was aimed to determine the relationship between PLR (platelet to lymphocyte ratio) and the lateral pillar classification of Perthes disease, and to provide an alternative index for clinical diagnosis. In addition, the association of the PLR with the necrosis stage of Perthes disease was also explored. This was a retrospective study. 74 children with Perthes disease and 60 children in the healthy control group without femoral head necrosis in our hospital from 2012 to 2021 were collected. The general data and clinical parameters were collected from the hospital information system. The modified herring lateral pillar classification was collected for the fragmentation stage case group and the PLR, NLR (neutrophil to lymphocyte ratio), LMR (lymphocyte to monocyte ratio) and PNR (platelet to neutrophil ratio) were calculated. The cases were divided into four groups, herring A and B were group I, herring B/C and C were group II, the healthy control group was group III, and the necrosis stage was group IV. The hematological indexes (NLR, PLR, LMR, PNR) of children at different stages were statistically analyzed. Group I consisted of 36 patients, with an average age of 7.4 ± 2.0 years (3–11 years). Group II consisted of 23 patients, with an average age of 7.4 ± 1.9 years (4–12 years). Group III consisted of 60 patients, with a mean age of 7.4 ± 2.7 years (4–13 years). Group IV consisted of 15 patients, with an average age of 6.4 ± 1.7 years (3–10 years). The average values of PLR in groups I, II, III and IV were 131.98 ± 47.44, 122.19 ± 37.88, 102.46 ± 30.68 and 128.90 ± 28.11, respectively. It's worth noting that there was statistically significant difference among groups I, II and III (P = 0.003). The optimal threshold of PLR was 130.25, the sensitivity was 45.8% and the specificity was 85%. PLR was also significantly different between groups III and group IV. PLR was higher in Herring A and B classifications than in Herring B/C and C classifications. PLR had certain diagnostic value in both the necrosis stage and fragmentation stage as a risk factor.

show abstract

Increased circulating CD31+/CD42b-EMPs in Perthes disease and inhibit HUVECs angiogenesis via endothelial dysfunction

Cited by 15 publications

References 36 publications

Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma

Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma

Microvesicles with mitochondrial content are increased in patients with sepsis and associated with inflammatory responses

Platelet to lymphocyte ratio was a risk factor in Perthes disease

Contact Info

Product

Resources

About