Increased chromosome X, Y, and 18 nondisjunction in sperm from infertile patients that were identified as normal by strict morphology: implication for intracytoplasmic sperm injection

Ryu, Hyun-Mee; Lin, Weigan; Lamb, Dolores J.; Chuang, Weber W.; Lipshultz, Larry I.; Bischoff, Farideh Z.

doi:10.1016/s0015-0282(01)02827-8

Cited by 64 publications

(35 citation statements)

References 26 publications

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“…Although some have demonstrated a correlation between such parameters and aneuploidy within subsets of subfertile men, there is little consistency between reports and data from our cohort demonstrates no significant correlation between any chromosome and sperm parameter. Ryu et al (2001) demonstrated that 3.3% of sperm from subfertile men selected as "normal" were aneuploid for chromosomes 18, X and Y. Burrello et al (2003) demonstrated high ICSI pregnancy rates in their cohort with high ARs following transfer of multiple embryos.…”

Section: Discussionmentioning

confidence: 99%

The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection?

et al. 2007

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BACKGROUND: We present the first powered prospective study to assess whether sperm aneuploidy can predict the outcome of ICSI. METHODS: Our null hypothesis was that aneuploidy rates (AR) are identical in men who achieve successful (Group A) and unsuccessful (Group B) ICSI outcome. A power calculation yielded a sample number of 56 to achieve 80% power to reject our hypothesis at the 5% significance level. Samples for testing were obtained on the day of embryo transfer and tests were performed on raw pre-preparation samples. Sperm AR of chromosomes 13, 18, 21, X/Y were assessed using fluorescence in-situ hybridization (FISH) techniques (mean of 1223 sperm). RESULTS: There was no significant difference in any patient, seminal, cycle or laboratory characteristic between groups that may have affected outcome. Total AR (2.37 versus 1.18%, P 5 0.01), as well as AR of chromosomes 18, X/Y and 18 1 X/Y (1.48 versus 0.67%, P 5 0.005) were significantly higher in Group B compared with Group A. Regression analysis confirmed these differences to be independent of other variables and showed a 2.6-fold change in odds of achieving a pregnancy for every 1% change in total AR. CONCLUSIONS: Our findings confirm a potential role for aneuploidy testing in the work-up of ICSI patients as a predictor of success, as well as in future genetic counselling. If confirmed, there may also be a place for a study of preimplantation genetic screening to improve ICSI success in men found to have high AR and ICSI failure.

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Section: Discussionmentioning

confidence: 99%

The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection?

et al. 2007

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“…The strict Tygerberg criteria for assessing sperm morphology recommended by WHO (2010) are based on the morphology of spermatozoa capable of penetrating cervical mucus and binding to the zona pellucida and therefore have a basis in biology. There is no known link between normal head morphology and the genetic quality of a spermatozoon (Menkveld et al, 1990(Menkveld et al, , 1991Ryu et al, 2001;Simon et al, 2010). This suggests that sperm DNA testing will add further information not available through a conventional semen analysis.…”

Section: Introductionmentioning

confidence: 99%

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Lewis

Aitken

Conner

et al. 2013

Reproductive BioMedicine Online

245

182

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Lewis, S.E.M. et al. (2013). The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Astract:Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency. IntroductionMale factor infertility is implicated in more than 40% of couples presenting for assisted reproduction treatment. Conventional semen analysis continues to be the only routine test to diagnose this condition even though it is known that such descriptive assessments cannot discriminate between the spermatozoa of fertile and infertile men (Guzick et al., 2001). The shifting values for normality (all 'normal' values now lower) in the fifth edition of the WHO manual (World Health Organization, 2010) compared with the previous WHO editions may result in even less men being classified as infertile (Murray et al., 2012).

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“…These data are supported by evaluating the aneuploidy rate in abnormal spermatozoa. The aneuploidy rates for chromosomes X, Y and 18 was 29% in morphologically abnormal spermatozoa compared to 1.8%-5.5% in morphologically normal spermatozoa from the same sample (Ryu et al 2004). …”

Section: Imsimentioning

confidence: 80%

Effectiveness of Assisted Reproduction Techniques as an Answer to Male Infertility

Chamayou¹,

Guglielmino²

2012

Male Infertility

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Increased chromosome X, Y, and 18 nondisjunction in sperm from infertile patients that were identified as normal by strict morphology: implication for intracytoplasmic sperm injection

Cited by 64 publications

References 26 publications

The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection?

The role of sperm aneuploidy as a predictor of the success of intracytoplasmic sperm injection?

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Effectiveness of Assisted Reproduction Techniques as an Answer to Male Infertility

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