Increased cell wall thickness of endodermis and protoxylem in Aeluropus littoralis roots under salinity: The role of LAC4 and PER64 genes

Movafeghi, Ali; Dehestani, Ali; Mehrabanjoubani, Pooyan

doi:10.1016/j.jplph.2017.08.002

Cited by 32 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several lines of evidence have demonstrated that salt stress and drought affect the width of Casparian strips and induce suberification of the endodermis in many plants species, suggesting a strategy to block the entry of toxic elements into the root vasculature and to prevent water and oxygen loss [51,52]. Indeed, our results indicate an increase in the thickness of the cell wall endodermis that match those reported in Gossypium hirsutum [53], Oriza sativa [54] and the facultative halophyte Aeluropus littoralis [55] under salinity conditions. Also, they match the findings of Vasellati et al [50] who reported the formation of a conspicuous endodermis in Paspalum dilatatum under drought stress.…”

Section: Effects Of Stress Conditions On Seedling Growth and Anatomicsupporting

confidence: 85%

Morpho-Physiological Responses of Alamo Switchgrass During Germination and Early Seedling Stage Under Salinity or Water Stress Conditions

et al. 2018

View full text Add to dashboard Cite

Switchgrass (Panicum virgatum L.) is a warm perennial grass with valuable characteristics as a biofuel crop. To avoid competition with food crops, biofuel crops will be likely relegated to less productive soils such as marginal lands. Consequently, the salinity and water scarcity problems that commonly affect marginal lands, compromise biofuel crops germination, emergence and seedling establishment. The aims of this study were to study the germination and seedling growth of switchgrass under salinity and water stress, and to describe the morpho-anatomical responses of the roots and leaves in the seedlings to these stresses. The effect of salt and water stress was assessed using sodium chloride (NaCl) and polyethylene glycol 8000 (PEG) at the same water potentials of-0.8,-1.0 and-1.2 MPa. Seeds were moist prechilled for 7 days at 5 ºC and germinated at 30ºC/15 ºC (8 h light/16 h dark). NaCl treatments (-0.8 and-1.0 MPa) delayed germination rates but did not reduce the final germination percentage, whereas at a lower potential (-1.2 MPa) the final germination percentage was diminished. The effects of PEG (-1.0 and-1.2 MPa) on the germination rate and final percentage, were more detrimental than those induced by iso-osmotic concentrations of NaCl. PEG and NaCl reduced significantly the vigor index of-0.8 to-1.2 MPa. The morpho-anatomical changes such as the reduction in the root cross-section area and the thickening of the endodermis walls for both stress conditions and aerenchyma formation in the cortex under salinity, could significantly contribute in the survival and tolerance during the early seedling stages.

show abstract

Section: Effects Of Stress Conditions On Seedling Growth and Anatomicsupporting

confidence: 85%

Morpho-Physiological Responses of Alamo Switchgrass During Germination and Early Seedling Stage Under Salinity or Water Stress Conditions

et al. 2018

View full text Add to dashboard Cite

show abstract

“…So, it can be concluded that the CCCH genes contain ABRE or DRE as the drought stress-responsive genes in their promoter sequences [30]. The zinc finger protein encoding transcription factors was previously reported as stress-inducible genes by Zouari et al [12].…”

Section: Discussionmentioning

confidence: 99%

“…Aeluropus littoralis is a halophytic plant of Poaceae family. It is a monocotyledonous halophyte which usually grows in the regions with intermediate to high salinity [11, 12]. A. littoralis can tolerate up to 600 mM NaCl [13] or 800 mM NaCl [11].…”

Section: Introductionmentioning

confidence: 99%

Exploiting Differential Gene Expression to Discover Ionic and Osmotic-Associated Transcripts in the Halophyte Grass Aeluropus littoralis

et al. 2019

View full text Add to dashboard Cite

Background Salinity as a most significant environmental challenges affects the growth and productivity of plants worldwide. In this study, the ionic and iso-osmotic effects of salt stress were investigated in Aeluropus littoralis L., a halophyte grass species from Poaceae family, by cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. To dissect the two different effects (ionic and osmotic) exerted by salt stress, various ionic agents including 200 and 400 mM sodium chloride (NaCl), 200 and 400 mM potassium chloride (KCl) as well as 280 and 406 gl − 1 (− 0.9 and − 1.4 MPa) polyethylene glycol 6000 (PEG) as their iso-osmotic concentrations were applied. Results Application of KCl and PEG significantly reduced the fresh weight (FW) of A. littoralis seedlings compared to control while NaCl treatment markedly enhanced the FW. At the transcriptome level, different observations of changes in gene expression have been made in response of A. littoralis to ionic and osmotic stresses. Out of 69 transcript derived fragments (TDFs), 42 TDFs belong to 9 different groups of genes involved in metabolism (11.6%), transcription (10.2%), ribosomal protein (8.7%), protein binding (8.7%) transporter (5.8%), translation (5.8%), signal transduction (4.3%), nucleosome assembly protein (2.9%) and catabolism (2.9%). The 44 and 28 percent of transcripts were expressed under ionic stress (NaCl-specific and KCl-specific) and osmotic stress (common with NaCl, KCl and PEG), respectively which indicating a greater response of plants to ionic stress than osmotic stress. Expression pattern of eight candidate TDFs including; SYP81 , CAND1 , KATN , ISB1 , SAMDC , GLY1 , HAK18 and ZF30 was evaluated by RT-qPCR at high salinity levels and recovery condition. Conclusion Differential regulation of these TDFs was observed in root and shoot which confirm their role in salt stress tolerance and provide initial insights into the transcriptome of A. littoralis . Expression pattern of ionic and osmotic-related TDFs at A. littoralis can be taken as an indication of their functional relevance at different salt and drought stresses.

show abstract

“…Downregulation of CCoAOMT gene was predicted to increase wood density in Populus trichocarpa by affecting lignin biosynthesis (Wang J. P. et al, 2018). Enhanced cell wall lignification in root cells is considered to be the salt tolerance feature in halophytes (Barzegargolchini et al, 2017). In addition, root aerenchyma structures that were formed in P. nuttalliana in response to NaCl may have an important role in improving root aeration under stress.…”

Section: Adaptations Of Plant Structure To Salinitymentioning

confidence: 99%

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

2021

View full text Add to dashboard Cite

Elevated soil salinity exacerbated by human activities and global climate change poses serious threats to plant survival. Although halophytes provide many important clues concerning salt tolerance in plants, some unanswered questions remain to be addressed, including the processes of water and solute transport regulation. We performed high-throughput RNA-sequencing in roots and metabolome characterizations in roots and leaves of Puccinellia nuttalliana halophytic grass subjected to 0 (control) and 150 mM NaCl. In RNAseq, a total of 31 Gb clean bases generated were de novo assembled into 941,894 transcripts. The PIP2;2 and HKT1;5 transcript levels increased in response to the NaCl treatment implying their roles in water and ion homeostasis. Several transcription factors, including WRKY39, DEK3, HY5, and ABF2, were also overexpressed in response to NaCl. The metabolomic analysis revealed that proline and dopamine significantly increased due to the upregulation of the pathway genes under salt stress, likely contributing to salt tolerance mechanisms. Several phosphatidylcholines significantly increased in roots suggesting that the alterations of membrane lipid composition may be an important strategy in P. nuttalliana for maintaining cellular homeostasis and membrane integrity under salt stress. In leaves, the TCA cycle was enriched suggesting enhanced energy metabolism to cope with salt stress. Other features contributing to the ability of P. nuttalliana to survive under high salinity conditions include salt secretion by the salt glands and enhanced cell wall lignification of the root cells. While most of the reported transcriptomic, metabolomics, and structural alterations may have consequences to water balance maintenance by plants under salinity stress, the key processes that need to be further addressed include the role of the changes in the aquaporin gene expression profiles in the earlier reported enhancement of the aquaporin-mediated root water transport.

show abstract

Increased cell wall thickness of endodermis and protoxylem in Aeluropus littoralis roots under salinity: The role of LAC4 and PER64 genes

Cited by 32 publications

References 33 publications

Morpho-Physiological Responses of Alamo Switchgrass During Germination and Early Seedling Stage Under Salinity or Water Stress Conditions

Morpho-Physiological Responses of Alamo Switchgrass During Germination and Early Seedling Stage Under Salinity or Water Stress Conditions

Exploiting Differential Gene Expression to Discover Ionic and Osmotic-Associated Transcripts in the Halophyte Grass Aeluropus littoralis

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

Contact Info

Product

Resources

About