Increased calpain content and progressive degradation of neurofilament protein in spinal cord injury

Banik, Naren L.; Matzelle, Denise; Gantt-Wilford, Gloria; Osborne, Anne H; Hogan, Edward L.

doi:10.1016/s0006-8993(96)01488-6

Cited by 105 publications

(65 citation statements)

References 26 publications

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“…PEA-15 is a phosphorylation-regulated astroglia protein. The PEA-15 protein is shown to regulate autophagy via activation of JNK in glioma cells (50). Its up-regulation suggests the involvement in astroglia responses in SCI.…”

Section: Discussionmentioning

confidence: 98%

Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury

Moghieb

Bramlett

Das

et al. 2016

Molecular & Cellular Proteomics

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Acute spinal cord injury (SCI) is a devastating condition with many consequences and no known effective treatment. Although it is quite easy to diagnose traumatic SCI, the assessment of injury severity and projection of disease progression or recovery are often challenging, as no consensus biomarkers have been clearly identified. Here rats were subjected to experimental moderate or severe thoracic SCI. At 24h and 7d postinjury, spinal cord segment caudal to injury center versus sham samples was harvested and subjected to differential proteomic analysis. Cationic/anionic-exchange chromatography, followed by 1D polyacrylamide gel electrophoresis, was used to reduce protein complexity. A reverse phase liquid chromatography-tandem mass spectrometry proteomic platform was then utilized to identify proteome changes associated with SCI. Twenty-two and 22 proteins were up-regulated at 24 h and 7 day after SCI, respectively; whereas 19 and 16 proteins are down-regulated at 24 h and 7 day after SCI, respectively, when compared with sham control. A subset of 12 proteins were identified as candidate SCI biomarkers -TF (

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Section: Discussionmentioning

confidence: 98%

Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury

Moghieb

Bramlett

Das

et al. 2016

Molecular & Cellular Proteomics

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“…compaction and membranous organelles begin to accumulate in paranodal regions as fast axonal transport mechanisms fail. Mitochondrial swelling has also been documented in the hyperacute phase (Banik et al, 1997;Maxwell, 1996). Combinations of these factors likely comprise significant intracellular contributions to ljj restriction after SCI.…”

Section: Discussionmentioning

confidence: 99%

Diffusion Tensor Imaging at 3 Hours after Traumatic Spinal Cord Injury Predicts Long-Term Locomotor Recovery

Kim

Loy

Wang

et al. 2010

Journal of Neurotrauma

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Accurate diagnosis of spinal cord injury (SCI) severity must be achieved before highly aggressive experimental therapies can be tested responsibly in the early phases after trauma. These studies demonstrate for the first time that axial diffusivity (ljj), derived from diffusion tensor imaging (DTI) within 3 h after SCI, accurately predicts long-term locomotor behavioral recovery in mice. Female C57BL=6 mice underwent sham laminectomy or graded contusive spinal cord injuries at the T9 vertebral level (5 groups, n ¼ 8 for each group). In-vivo DTI examinations were performed immediately after SCI. Longitudinal measurements of hindlimb locomotor recovery were obtained using the Basso mouse scale (BMS). Injured and spared regions of ventrolateral white matter (VLWM) were reliably separated in the hyperacute phase by threshold segmentation. Measurements of ljj were compared with histology in the hyperacute phase and 14 days after injury. The spared normal VLWM determined by hyperacute ljj and 14-day histology correlated well (r ¼ 0.95). A strong correlation between hindlimb locomotor function recovery and ljj-determined spared normal VLWM was also observed. The odds of significant locomotor recovery increased by 18% with each 1% increase in normal VLWM measured in the hyperacute phase (odds ratio ¼ 1.18, p ¼ 0.037). The capability of measuring subclinical changes in spinal cord physiology and murine genetic advantages offer an early window into the basic mechanisms of SCI that was not previously possible. Although significant obstacles must still be overcome to derive similar data in human patients, the path to clinical translation is foreseeable and achievable.

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“…Initially, marked decreases in the distance between neurofilaments result in cytoskeletal collapse and compaction (Gallyas et al, 2002). Mitochondria swell and Ca 2ϩ influx activates the neutral protease, calpain, leading to neurofilament and microtubule degradation (Maxwell, 1996;Banik et al, 1997). The ensuant breakdown in fast axonal transport causes accumulation of membranous or-LOY ET AL.…”

Section: Discussionmentioning

confidence: 99%

Diffusion Tensor Imaging Predicts Hyperacute Spinal Cord Injury Severity

Loy

Kim

Xie

et al. 2007

Journal of Neurotrauma

118

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"Diffusion tensor imaging predicts hyperacute spinal cord injury severity." Journal of Neurotrauma.24,6. 979-990. (2007 We recently demonstrated that in vivo derived diffusion tensor imaging (DTI) parameters are sensitive and specific biomarkers for spinal cord white matter damage. In this study, non-invasive in vivo DTI was utilized to evaluate the white matter of C57BL/6 mice 3 h after mild (0.3 mm), moderate (0.6 mm), or severe (0.9 mm) contusive SCI. In the hyperacute phase, relative anisotropy maps provided excellent gray-white matter contrast in all degrees of injury. In vivo DTI-derived measurements of axial diffusion differentiated between mild, moderate, and severe contusive SCI with good histological correlation. Cross-sectional regional measurements of white matter injury severity between dorsal columns and VWM varied with increasing cord displacement in a pattern consistent with spinal cord viscoelastic properties.

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Increased calpain content and progressive degradation of neurofilament protein in spinal cord injury

Cited by 105 publications

References 26 publications

Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury

Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury

Diffusion Tensor Imaging at 3 Hours after Traumatic Spinal Cord Injury Predicts Long-Term Locomotor Recovery

Diffusion Tensor Imaging Predicts Hyperacute Spinal Cord Injury Severity

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